The macro- (Ca, Mg, Na, and K) and trace (Fe, Cu, Mn, and Zn) element standards (CaCO3, Mg band, NaCl, KCl, Fe(NO3)3, Cu(NO3)2, Mn(NO3)2, and Zn(NO3)2; >99% purity), as well as LaCl2 and CsCl (>99% purity) were purchased from Merck (Darmstadt, Germany). Pepsin (from porcine stomach mucosa; 1.470 units/mg prot; P-7000); pancreatin (from porcine pancreas, activity equivalent to 4 x U.S.P. specifications; P-1750); and porcine bile (B-8631) were purchased from Sigma-Aldrich Co. (St. Louis, MO, USA). All other reagents were purchased from Panreac Química S.L.U. (Barcelona, Spain). Water was treated in a Milli-Q water purification system (TGI Pure Water Systems, Indianapolis, IN, USA).
Lacl 2
Manufactured by Merck Group
Sourced in Germany
LaCl2 is a chemical compound consisting of lanthanum and chloride. It is a crystalline solid with a melting point of 867°C. LaCl2 is commonly used as a laboratory reagent for various analytical and research applications.
Automatically generated - may contain errors
Lab products found in correlation
Acetonitrile, by Thermo Fisher Scientific (2 mentions)
Ethyl acetate, by Thermo Fisher Scientific (2 mentions)
N hexane, by Thermo Fisher Scientific (2 mentions)
Sodium chloride (nacl), by Merck Group (1 mentions)
Potassium chloride (kcl), by Merck Group (1 mentions)
Pepsin, by Merck Group (1 mentions)
Pancreatin, by Merck Group (1 mentions)
Caco3, by Merck Group (1 mentions)
Porcine bile b 8631, by Merck Group (1 mentions)
Cu no3 2, by Merck Group (1 mentions)
Mn no3 2, by Merck Group (1 mentions)
Zn no3 2, by Merck Group (1 mentions)
Fe no3 3, by Merck Group (1 mentions)
Fatty acids methyl ester reference standard mixture 37 standard 47885 u, by Merck Group (1 mentions)
Racemic tocol, by Matreya (1 mentions)
Tdf 100a kit, by Merck Group (1 mentions)
2 2 diphenyl 1 picrylhydrazyl (dpph), by Thermo Fisher Scientific (1 mentions)
Formic acid, by Avantor (1 mentions)
Phenolic standards, by Extrasynthese (1 mentions)
Fatty acids methyl ester reference standard mixture standard 47885 u, by Merck Group (1 mentions)
4 protocols using lacl 2
1
Macro and Trace Element Standards
2
Comprehensive Analytical Protocols for Food
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Acetonitrile 99.9%, n-hexane 95% and ethyl acetate 99.8% were of HPLC grade from Fisher Scientific (Lisbon, Portugal) . The fatty acids methyl ester (FAME) reference standard mixture 37 (standard 47885-U) was purchased from Sigma (St. Louis, MO, USA), as also other individual fatty acid isomers and standards of sugars (D-(+)mannitol, D-(+)-trehalose), tocopherols (α-, β-, and γ-isoforms), organic acids (oxalic and fumaric acids), phenolic compounds (gallic, p-hydroxybenzoic, protocatechuic, cinnamic and p-coumaric acids), trolox (6-hydroxy-2,5,7,8-tetramethylchroman-2carboxylic acid) , and dietary fiber enzyme kit (TDF-100A Kit), pepsin enzyme (P-7000), pancreatin enzyme (P-1750 ) and porcine bile (B-8631). Racemic tocol, 50 mg/mL, was purchased from Matreya (PA, USA). Micro (Fe, Cu, Mn and Zn) and macroelements (Ca, Mg, Na and K) standards (> 99% purity), as well LaCl 2 and CsCl (> 99% purity) were purchased from Merck (Darmstadt, Germany). Anthrone was obtained from Panreac Química (Barcelona, Spain). Water was treated in a Milli-Q water purification system (TGI Pure Water Systems, Greenville, SC, USA).
3
Analysis of Fatty Acids, Vitamins, and Minerals
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Acetonitrile, n-hexane and ethyl acetate were of HPLC grade from Fisher Scientific (Lisbon, Portugal). Formic acid was purchased from Prolabo (VWR International, France). Fatty acids methyl ester (FAME) reference standard mixture (standard 47885-U) was purchased from Sigma-Aldrich (St Louis, MO, USA), as well as trolox (6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid), L-ascorbic acid, tocopherols, sugar and organic acid standards, nitric acid, and hydrochloric acid, 5-CH 3 -H 4 folate monoglutamate (ref. 16252; Schircks laboratories, Jona, Switzerland), pteroyl diglutamic acid (ref. 16235; Schircks laboratories, Jona, Switzerland), pancreatic chicken homogenate (Pel Freez, Rogers, Arkansas), rat serum, NaBH 4 , formaldehyde and octanol. Micro and macroelement standards (>99% purity), as well as LaCl 2 and CsCl (>99% purity) were purchased from Merck (Darmstadt, Germany). Phenolic standards were from Extrasynthèse (Genay, France). 2,2-Diphenyl-1-picrylhydrazyl (DPPH) was obtained from Alfa Aesar (Ward Hill, MA, USA). All other general laboratory reagents were purchased from Panreac Química S.L.U. (Barcelona, Spain). Water was treated in a Milli-Q water purification system (TGI Pure Water Systems, Greenville, SC, USA).
4
Standardized Reagents and Media Protocol
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Micro (Fe, Cu, Mn and Zn) and macroelement (Ca, Mg, Na and K) standards (>99% purity), as well as LaCl 2 and CsCl (>99% purity) were purchased from Merck (Darmstadt, Germany). Mueller-Hinton agar (MH) and malt agar (MA) were obtained from the Institute of Immunology and Virology, Torlak (Belgrade, Serbia). Acetic acid, ellipticine, phosphate buffered saline (PBS), Acetic acid, sulforhodamine B (SRB), trichloroAcetic acid (TCA), Tris, streptomycin (Sigma-Aldrich S6501) and ampicillin (Sigma-Aldrich A9393) were purchased from Sigma (St Louis, MO, USA). Bifonazole (Srbolek, Belgrade, Serbia) and ketoconazole (Zorkapharma, Šabac, Serbia) were used as the reference fungicides.
Foetal bovine serum (FBS), L-glutamine, Hank's balanced salt solution (HBSS), trypsin-EDTA (ethylenediamine tetraAcetic acid), nonessential amino acid solution (2 mM), penicillin/streptomycin solution (100 U mL -1 and 100 mg mL -1 , respectively), RPMI-1640 and DMEM media were from Hyclone (Logan, UT, USA). Dimethylsulfoxide (DMSO), (Merck KGaA, Darmstadt, Germany) was used as a solvent. Water was treated in a Milli-Q water purification system (TGI Pure Water Systems, Greenville, SC, USA). All other chemicals and solvents were of analytical grade and purchased from common sources.
Foetal bovine serum (FBS), L-glutamine, Hank's balanced salt solution (HBSS), trypsin-EDTA (ethylenediamine tetraAcetic acid), nonessential amino acid solution (2 mM), penicillin/streptomycin solution (100 U mL -1 and 100 mg mL -1 , respectively), RPMI-1640 and DMEM media were from Hyclone (Logan, UT, USA). Dimethylsulfoxide (DMSO), (Merck KGaA, Darmstadt, Germany) was used as a solvent. Water was treated in a Milli-Q water purification system (TGI Pure Water Systems, Greenville, SC, USA). All other chemicals and solvents were of analytical grade and purchased from common sources.
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