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Ab195352 antibody

Manufactured by Abcam
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Ab195352 is a recombinant monoclonal antibody that recognizes the target protein. The antibody is produced in a mammalian cell line and is purified by affinity chromatography. The product is supplied as a liquid in buffer solution.

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Lab products found in correlation

Sc 374280 antibody, by Santa Cruz Biotechnology (2 mentions) Magnetic protein a g beads, by Thermo Fisher Scientific (2 mentions) Veriblot reagent, by Abcam (2 mentions) Complete mini edta free protease inhibitor cocktail, by Roche (2 mentions)

2 protocols using ab195352 antibody

1

Immunoprecipitation of WTAP and METTL3

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells intended for immunoprecipitation were lysed in lysis buffer containing 1% Triton-X in TBS with cOmplete mini EDTA free protease inhibitor cocktail (Roche). The lysate was diluted ½ in water before the addition of the IP antibody. WTAP was immunoprecipitated using sc-374280 antibody (Santa Cruz Biotechnology, Inc.), METTL3 was immunoprecipitated using ab195352 antibody (Abcam). After 4 h of incubation, the lysate/antibody mixture was added to magnetic protein A/G beads (ThermoFisher). After another 4 h of incubation, beads were washed 5 times using wash buffer containing 450 mM NaCl and 50 mM Tris pH 7.5, and boiled in Laemmli Buffer. Immunoprecipitates were analyzed using Western blotting as described above. If the detection antibody was raised in the same species as the immunoprecipitation antibody, blots were detected using Veriblot reagent (Abcam).
Jansens R.J., Verhamme R., Mirza A.H., Olarerin-George A., Van Waesberghe C., Jaffrey S.R, & Favoreel H.W. (2022). Alphaherpesvirus US3 protein-mediated inhibition of the m6A mRNA methyltransferase complex. Cell reports, 40(3), 111107.
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2

Immunoprecipitation of WTAP and METTL3

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells intended for immunoprecipitation were lysed in lysis buffer containing 1% Triton-X in TBS with cOmplete mini EDTA free protease inhibitor cocktail (Roche). The lysate was diluted ½ in water before the addition of the IP antibody. WTAP was immunoprecipitated using sc-374280 antibody (Santa Cruz Biotechnology, Inc.), METTL3 was immunoprecipitated using ab195352 antibody (Abcam). After 4 h of incubation, the lysate/antibody mixture was added to magnetic protein A/G beads (ThermoFisher). After another 4 h of incubation, beads were washed 5 times using wash buffer containing 450 mM NaCl and 50 mM Tris pH 7.5, and boiled in Laemmli Buffer. Immunoprecipitates were analyzed using Western blotting as described above. If the detection antibody was raised in the same species as the immunoprecipitation antibody, blots were detected using Veriblot reagent (Abcam).
Jansens R.J., Verhamme R., Mirza A.H., Olarerin-George A., Van Waesberghe C., Jaffrey S.R, & Favoreel H.W. (2022). Alphaherpesvirus US3 protein-mediated inhibition of the m6A mRNA methyltransferase complex. Cell reports, 40(3), 111107.
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