Pepsin (2000 FIP-U/g) was obtained from Merck (Darmstadt, Germany). Amyloglucosidase (14 IU/mg) was from Roche (Manheim, Germany). Pancreatin, αamylase (17.5 IU/mg), ABTS (2, 5, 7, and gallic acid were all obtained from Sigma-Aldrich (St. Louis, MO, USA). 2,4,6-Tris(2-pyridyl)-s-triazine (TPTZ) was from Fluka Chemicals (Madrid, Spain). Iron III-chlorure-6-hydrate were from Panreac (Castellar del Vallés, Barcelona, Spain).
Abts 2 5 7
Manufactured by Merck Group
Sourced in United States
ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid)) is a chromogenic substrate used in enzyme-linked immunosorbent assays (ELISA) and other colorimetric detection methods. It serves as an electron donor, accepting electrons from enzymes such as horseradish peroxidase (HRP) or alkaline phosphatase, resulting in the production of a colored product that can be measured spectrophotometrically.
Automatically generated - may contain errors
Lab products found in correlation
Gallic acid, by Merck Group (2 mentions)
Pepsin, by Merck Group (1 mentions)
α amylase, by Merck Group (1 mentions)
Pancreatin, by Merck Group (1 mentions)
Iron 3 chlorure 6 hydrate, by ITW Reagents (1 mentions)
Amyloglucosidase, by Roche (1 mentions)
Sulfuric acid, by Merck Group (1 mentions)
Catechin, by Merck Group (1 mentions)
Caffeic acid, by Merck Group (1 mentions)
Chlorogenic acid, by Merck Group (1 mentions)
Ferulic acid, by Merck Group (1 mentions)
Quercetin, by Merck Group (1 mentions)
Vanillin, by Merck Group (1 mentions)
Linoleic acid, by Vetec (1 mentions)
Methanol, by Vetec (1 mentions)
Folin ciocalteu reagent, by Dinâmica (1 mentions)
Acetone, by Vetec (1 mentions)
Acetic acid, by Vetec (1 mentions)
Gallic acid, by Vetec (1 mentions)
Chloroform, by Vetec (1 mentions)
2 protocols using abts 2 5 7
1
Reagents for enzymatic analysis
2
Antioxidant Characterization of Phenolic Compounds
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The chemical reagents ABTS ((2, 5, 7, Tween 40, and phenolic compound standards (gallic acid, catechin, chlorogenic acid, caffeic acid, ferulic acid, vanillin, quercetin, were obtained from Sigma-Aldrich (Saint Louis, Missouri, USA). methanol (HPLC grade) was obtained from J-TBacker (Alfragide, Lisbon, Portugal). In turn, the Folin-Ciocalteu reagent was obtained from Dinâmica (Indaiatuba, São Paulo, Brazil). Acetone, acetic acid, gallic acid, linoleic acid, methanol, and chloroform were obtained from Vetec (Saint Louis, Missouri, USA), while sulfuric acid was obtained from Merck (Darmstadt, Hessen, Germany). Ethanol, was obtained from Synth (Diadema, São Paulo, Brazil), while n-hexane and potassium persulfate were obtained from Neon (São Paulo, São Paulo, Brazil). All reagents were analytical grade, and stock and buffer solutions were prepared using distilled water.
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