The method of obtaining microbubbles was according to the protocol of a previous study (16 (link)). It was performed by sonication of 0.4 mg/ml 1,2-distearoyl-3-trimethylammoniumpropane (Avanti Polar Lipids, Inc.) with 1 mg/ml polyethyleneglycol-2000 stearate (Avanti Polar Lipids, Inc.), 2 mg/ml distearoylphosphatidylcholine (Avanti Polar Lipids, Inc.) and perfluoropropane gas. miR-4284 inhibitor (5'-AUGGGGUAUGUGAGCCC-3') or non-targeting inhibitor-NC (5'-CAGUACUUUUGUGUAGUACAA-3') was incubated with the microbubbles for 30 min at 37˚C. According to the manufacturer's protocol, the mixtures were added to A549 and H460 cells and transfected with Lipofectamine 3000 (Invitrogen; Thermo Fisher Scientific, Inc.).
Polyethyleneglycol 2000 stearate
Manufactured by Avanti Polar Lipids
Polyethyleneglycol-2000 stearate is a non-ionic surfactant produced by Avanti Polar Lipids. It is a polyethylene glycol (PEG) derivative with a molecular weight of approximately 2,000 Daltons and a stearic acid ester group. This compound is used as an emulsifier, solubilizer, and wetting agent in various pharmaceutical, cosmetic, and industrial applications.
Automatically generated - may contain errors
3 protocols using polyethyleneglycol 2000 stearate
1
Microbubble-Mediated miR-4284 Inhibitor Delivery
2
Microbubble-Mediated Delivery of miR-492 Inhibitor
Check if the same lab product or an alternative is used in the 5 most similar protocols
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In the experiment, the microbubbles were obtained by ultrasonic treatment. The microbubbles were performed by sonication of 0.4 mg/ml 1,2-distearoyl-3-trimethylammoni-umpropane (Avanti Polar Lipids, Inc.) with 1 mg/ml polyethyleneglycol-2000 stearate (Avanti Polar Lipids, Inc.), 2 mg/ml distearoylphosphatidylcholine (Avanti Polar Lipids, Alabaster, Inc.) and perfluoropropane gas, the ultrasonic irradiation intensity was 0.5 W/cm2 and the irradiation time was 60 s. miR-492 inhibitor or inhibitor NC was incubated with the microbubbles for 30 min at 37 °C. The mixture was added to A549 and PC9 cells and transfected with Lipofectamine 3000 (Invitrogen; Thermo Fisher Scientific, Inc.), then ultrasound irradiation was applied with an irradiation intensity of 0.5 W/cm2.
3
Microbubble-Mediated Delivery of miR-492 Inhibitor
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
In the experiment, the microbubbles were obtained by ultrasonic treatment. The microbubbles were performed by sonication of 0.4 mg/ml 1,2-distearoyl-3-trimethylammoni-umpropane (Avanti Polar Lipids, Inc.) with 1 mg/ml polyethyleneglycol-2000 stearate (Avanti Polar Lipids, Inc.), 2 mg/ml distearoylphosphatidylcholine (Avanti Polar Lipids, Alabaster, Inc.) and perfluoropropane gas, the ultrasonic irradiation intensity was 0.5 W/cm2 and the irradiation time was 60 s. miR-492 inhibitor or inhibitor NC was incubated with the microbubbles for 30 min at 37 °C. The mixture was added to A549 and PC9 cells and transfected with Lipofectamine 3000 (Invitrogen; Thermo Fisher Scientific, Inc.), then ultrasound irradiation was applied with an irradiation intensity of 0.5 W/cm2.
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