The Huh-7 hepatocarcinoma cell line was generously provided by Dr. Sonia Zúñiga (CNB-Spanish National Centre for Biotechnology, Madrid, Spain). This cell line was obtained from a liver tumor in a 57-year-old Japanese male and was used to test HCoV-229E infection [33 (link)]. The Vero cell line (ATCC-CCL81), which was derived from the kidney of an adult African green monkey (Cercopithecus aethiops) was purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA). All cell lines were cultured in growth medium (GM) containing low-glucose Dulbecco’s modified Eagle medium (Life Technologies, Paisley, UK) supplemented with 5% fetal bovine serum (FBS), 1% (v/v) penicillin G (50 U/mL) and streptomycin (50 µg/mL) (HyClone Laboratories, Logan, UT, USA). Cells were maintained at 37 °C, in a humidified atmosphere of 5% CO2 (Heraeus HERAcell, Thermofisher. Waltham, MA, USA).
Vero cell line atcc ccl81
Manufactured by American Type Culture Collection
Sourced in United States
The Vero cell line (ATCC-CCL81) is a continuous cell line derived from the kidney of the African green monkey (Cercopithecus aethiops). This cell line is commonly used in cell culture and research applications.
Automatically generated - may contain errors
Lab products found in correlation
Low glucose dulbecco s modified eagle medium, by Thermo Fisher Scientific (1 mentions)
Heraeus heracell, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Cytiva (1 mentions)
Streptomycin, by Cytiva (1 mentions)
Penicillin g, by Cytiva (1 mentions)
Cercopithecus aethiops, by American Type Culture Collection (1 mentions)
2 protocols using vero cell line atcc ccl81
1
Cell Culture of Huh-7 and Vero Cells
2
Evaluating Quinoxalinones' Effects on Cancer Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Human erythroleukemia
TF1 (ATCC
CRL-2003) and HEL 92.1.7 (ATCC
TIB-180) cell lines, human liver cancer HepG2 cell line (ATCC HB-8065)
and monkey (Cercopithecus aethiops) kidney Vero cell
line (ATCC CCL-81) were purchased from American Type Culture Collection
(ATCC, Manassas, VA, USA). Primary antibodies against JAK2 (ab108596),
p-JAK2 (ab32101; Y1007/1008) were purchased from Abcam. Primary antibody
of p-STAT5 (#9351; Y694), β-actin (#4970) and secondary antibody,
antirabbit IgG, HRP-linked (#7074) were purchased from Cell Signaling
Technology. Poly(glu-tyr) Peptide(P61–58) was purchased from
SignalChem Biotech (Canada). Muse annexin V and dead cell kit (MCH100105)
and caspase-3/7 kit (MCH100108) were purchased from Merk (Germany).
Quinoxalinones were synthesized and obtained from Associate Professor
Dr. Sirilata Yotphan, Department of Chemistry, Faculty of Science,
Mahidol University.26 (link)−28 (link)
TF1 (ATCC
CRL-2003) and HEL 92.1.7 (ATCC
TIB-180) cell lines, human liver cancer HepG2 cell line (ATCC HB-8065)
and monkey (Cercopithecus aethiops) kidney Vero cell
line (ATCC CCL-81) were purchased from American Type Culture Collection
(ATCC, Manassas, VA, USA). Primary antibodies against JAK2 (ab108596),
p-JAK2 (ab32101; Y1007/1008) were purchased from Abcam. Primary antibody
of p-STAT5 (#9351; Y694), β-actin (#4970) and secondary antibody,
antirabbit IgG, HRP-linked (#7074) were purchased from Cell Signaling
Technology. Poly(glu-tyr) Peptide(P61–58) was purchased from
SignalChem Biotech (Canada). Muse annexin V and dead cell kit (MCH100105)
and caspase-3/7 kit (MCH100108) were purchased from Merk (Germany).
Quinoxalinones were synthesized and obtained from Associate Professor
Dr. Sirilata Yotphan, Department of Chemistry, Faculty of Science,
Mahidol University.26 (link)−28 (link)
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