Nci a549

Manufactured by American Type Culture Collection

Sourced in United States

The NCI-A549 is a type of immortalized cell line derived from human lung carcinoma. It is commonly used in various research applications, such as the study of cancer biology and drug development.

Automatically generated - may contain errors

Lab products found in correlation

8 protocols using nci a549

Isogenic Cell Line Generation for Cancer Research

Check if the same lab product or an alternative is used in the 5 most similar protocols

All cell lines were maintained in RPMI supplemented with 10% FBS and incubated at 37C with 5% CO₂. The following cell lines were used in this study: NCI-H358 (ATCC Cat# CRL-5807, RRID:CVCL_1559), NCI-H292 (ATCC Cat# CRL-1848, RRID:CVCL_0455), NCI-A549 (ATCC Cat# CRM-CCL-185, RRID:CVCL_0023), NCI-H460 (ATCC Cat# HTB-177, RRID:CVCL_0459). Identity of cell lines were confirmed by STR profiling, and all lines tested mycoplasma negative. Isogenic A549 and H460 lines reconstituted with pInducer20-GFP (RRID:Addgene_44012), pInducer20-LKB1 (product of RRID:Addgene_44012 and RRID:Addgene_82320), or pInducer20-KEAP1 (product of RRID:Addgene_44012 and RRID:Addgene_81925) were obtained by lentiviral transduction under Neomycin selection (1000ug/mL). These lentiviral vectors were engineered using a gateway cloning protocol (Gateway LR Clonase II, Invitrogen, cat# 11791–020).

Wohlhieter C.A., Richards A.L., Uddin F., Hulton C.H., Quintanal-Villalonga À., Martin A., de Stanchina E., Bhanot U., Asher M., Shah N.S., Hayatt O., Buonocore D.J., Rekhtman N., Shen R., Arbour K.C., Donoghue M., Poirier J.T., Sen T, & Rudin C.M. (2020). Concurrent Mutations in STK11 and KEAP1 Promote Ferroptosis Protection and SCD1 Dependence in Lung Cancer. Cell reports, 33(9), 108444.

+ Open protocol

+ Expand

Lung Cancer Cell Line Cultivation

Check if the same lab product or an alternative is used in the 5 most similar protocols

Human lung cancer cell lines NCI-H460, NCI-H510, and NCI-A549 were purchased from ATCC (Manassas, VA). NCI-H460 cells were maintained in RPMI-1640 medium with 10% fetal bovine serum (FBS). The NCI-H510 and NCI-A549 cells were maintained in F-12K medium with 10% FBS. All cells were grown at 37°C with 5% CO₂ in air. THS-laden DMEM was prepared as above and diluted in complete culture medium. The culture medium was replaced every three days with fresh THS or control supplemented culture media.

Hang B., Wang Y., Huang Y., Wang P., Langley S.A., Bi L., Sarker A.H., Schick S.F., Havel C., Jacob P I.I.I., Benowitz N., Destaillats H., Tang X., Xia Y., Jen K.Y., Gundel L.A., Mao J.H, & Snijders A.M. (2018). Short-term early exposure to thirdhand cigarette smoke increases lung cancer incidence in mice. Clinical Science (London, England : 1979), 132(4), 475-488.

+ Open protocol

+ Expand

Isogenic Cell Line Generation for Cancer Research

Check if the same lab product or an alternative is used in the 5 most similar protocols

+ Open protocol

+ Expand

CAMSAP3 Knockout in NSCLC Cell Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols

NSCLC NCI‐H460 and NCI‐A549 cells were purchased from American Type Culture Collection (ATCC). CAMSAP3 knockout H460 (H460/C3ko) and control (H460/Ctrl) cells were generated as previously reported.¹⁹ The antibody against CAMSAP3 was a gift from Professor Takeihi Masatoshi (RIKEN) and others were provided in Table S2.

Wattanathamsan O., Chetprayoon P., Chantaravisoot N., Wongkongkathep P., Chanvorachote P, & Pongrakhananon V. (2021). CAMSAP3 depletion induces lung cancer cell senescence‐associated phenotypes through extracellular signal‐regulated kinase inactivation. Cancer Medicine, 10(24), 8961-8975.

+ Open protocol

+ Expand

Cell Culture Protocols for Cancer Research

Check if the same lab product or an alternative is used in the 5 most similar protocols

Human lung cancer cells (NCI-A549, NCI-H460, SK-MES-1, NCI-H1299, and NCI-H1975), normal lung cells (BEAS-2B), and breast cancer cells (MBA-MD-231 and MCF-7) were purchased from the American Type Culture Collection (ATCC). Human colon cancer cells (HCT-116, SW620, SW480, and RKO), cervical cancer cells (Hela), liver cancer cells (HEPG2), and HEK293T cells were also purchased from ATCC. SW480 was cultured in L-15 supplemented with 10% fetal bovine serum (FBS). Human lung cancer cells were cultured in RPMI-1640 supplement with 10% FBS. Unless otherwise stated, all other cells were maintained in DMEM supplemented with FBS.

Wu J., Chen Y., Li R., Guan Y., Chen M., Yin H., Yang X., Jin M., Huang B., Ding X., Yang J., Wang Z., He Y., Wang Q., Luo J., Wang P., Mao Z., Huen M.S., Lou Z., Yuan J, & Gong F. (2023). Synergistic anticancer effect by targeting CDK2 and EGFR–ERK signaling. The Journal of Cell Biology, 223(1), e202203005.

+ Open protocol

+ Expand

Antitumor Activity Evaluation of Compounds

Check if the same lab product or an alternative is used in the 5 most similar protocols

The new prepared candidates 5a–5n were evaluated for their antitumor activities against four selected cell lines, the adenocarcinomic human alveolar basal epithelial cells NCI-A549, NCI-H1975, human pulmonary adenocarcinoma PC9, and the caucasian fibroblast-like fetal lung cells WI-38 (purchased from American Type Culture Collection (ATCC), United States of America), using an MTT assay [26 (link)], as described earlier [27 (link)].

Hassanin M.A., Mustafa M., Abourehab M.A., Hassan H.A., Aly O.M, & Beshr E.A. (2022). Design and Synthesis of New Hydantoin Acetanilide Derivatives as Anti-NSCLC Targeting EGFRL858R/T790M Mutations. Pharmaceuticals, 15(7), 857.

+ Open protocol

+ Expand

Establishment of NSCLC Cell Lines and PDX Models

Check if the same lab product or an alternative is used in the 5 most similar protocols

Human NSCLC cell lines, NCI-A549, NCI-H1395, NCI-H157, NCI-H1650, and NCI-H358, were obtained from the American Type Culture Collection (Manassas, VA). All cells were cultured in RPMI medium supplemented with 10% fetal bovine serum (Hyclone Europe, Ltd., Cramlington, United Kingdom) and antibiotics at 37°C and 5% carbon dioxide. Authentication of human cell lines was performed by short tandem repeat DNA profiling analysis.
NSCLC PDX models were established using protocols approved by the University Health Network Research Ethics Board and Animal Care Committee as previously described. 21 Human lung cell lines (HLC) 12, 277, 137, and 196 were derived from tumor fragments of PDX models grown in NOD-SCID mice 21 and were processed into a single-cell suspension to establish long-term cell culture growth on plastic dishes. Tumor fragments were minced, digested in collagenase for 1 hour at 37°C, and treated with DNase for 10 minutes at 37°C. Magnetic columns (MACS Technology) coated with H2Kb/H2Dd antimouse antibodies were used to deplete stromal mouse fibroblast cells in the tumor cell population. RG7388 and solvents were provided by Hoffmann-La Roche (Nutley, NJ).

Hai J., Sakashita S., Allo G., Ludkovski O., Ng C., Shepherd F.A, & Tsao M.S. (2015). Inhibiting MDM2-p53 Interaction Suppresses Tumor Growth in Patient-Derived Non-Small Cell Lung Cancer Xenograft Models. Journal of thoracic oncology : official publication of the International Association for the Study of Lung Cancer, 10(8).

+ Open protocol

+ Expand

Cell Line Authentication and Maintenance

Check if the same lab product or an alternative is used in the 5 most similar protocols

NCI-A549, NCI-H23, NCI-H1792, NCI-H441, H460, and NCI-H358 cells were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA). NCI-SW1573, NCI-H2030, AsPC-1, Panc 02.03, Panc 10.05, and H1944 cells were obtained from Cobioer (Nanjing, China). All cell lines were routinely authenticated by short tandem repeat analysis (Genesky Biotechnologies, Shanghai, China) and were maintained in the appropriate culture medium suggested by the suppliers.

Chen Y.H., Lv H., Shen N., Wang X.M., Tang S., Xiong B., Ding J., Geng M.Y, & Huang M. (2019). EPHA2 feedback activation limits the response to PDEδ inhibition in KRAS-dependent cancer cells. Acta Pharmacologica Sinica, 41(2), 270-277.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!