Murine LLC, 4T1 murine mammary adenocarcinoma, and A549 human lung carcinoma cell lines were purchased from the American Type Culture Collection (ATCC) and used within 6 months after thawing. LM2-4, a metastatic variant of the MDA-MB-231 human breast carcinoma line (Munoz et al., 2006 (link)), was kindly provided by Dr. R.S. Kerbel (Toronto, ON, Canada). LLC cells were grown in DMEM. 4T1, A549, and LM2-4 cells were grown in RPMI-1640 medium. Culture media were supplemented with 10% fetal calf serum, 1% L-glutamine, 1% sodium pyruvate, and 1% streptomycin, penicillin, and neomycin in solution (10 mg/mL; Biological Industries). LECs were purchased from Lonza and cultured on fibronectin-coated plates (10 μg/mL; Biological Industries) with endothelial cell basal medium (EGM; Lonza).
4t1 murine mammary adenocarcinoma
Manufactured by American Type Culture Collection
4T1 murine mammary adenocarcinoma is a cell line derived from a spontaneously arising mammary tumor in a BALB/c mouse. It is a well-established model for studying breast cancer.
Automatically generated - may contain errors
Lab products found in correlation
L glutamine, by Sartorius (2 mentions)
Fetal calf serum (fcs), by Sartorius (1 mentions)
Penicillin, by Sartorius (1 mentions)
Streptomycin, by Sartorius (1 mentions)
Neomycin, by Sartorius (1 mentions)
Fetal bovine serum (fbs), by Sartorius (1 mentions)
Dulbecco s modified eagle medium, by Merck Group (1 mentions)
Ez pcr mycoplasma test kit, by Sartorius (1 mentions)
Streptomycin penicillin neomycin solution, by Sartorius (1 mentions)
Mcf7 human breast carcinoma, by American Type Culture Collection (1 mentions)
Emt6 murine breast carcinoma, by American Type Culture Collection (1 mentions)
2 protocols using 4t1 murine mammary adenocarcinoma
1
Establishing In Vitro Models for Cancer Research
2
Breast Cancer Cell Line Cultivation
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EMT6 murine breast carcinoma, 4T1 murine mammary adenocarcinoma, and MCF7 human breast carcinoma cell lines were purchased from the American Type Culture Collection, and were used within 6 months after resuscitation. The EMT6-F2 cell line, a metastatic variant of EMT6, was generated in our lab in a similar way that was previously described for MDA-MB-231 human breast carcinoma cells (21 (link)). All cell lines were cultured in Dulbecco's modified Eagle medium (Sigma-Aldrich) supplemented with 10% fetal bovine serum (Biological Industries), 1% L-glutamine, 1% sodium pyruvate, and 1% streptomycin–penicillin–neomycin solution (Biological Industries). Cells were cultured at 37°C in a humidified atmosphere containing 5% CO2. Some of the cell lines were stably transfected with a GFP-expressing vector (Clontech Laboratories, 632379). Cells were routinely tested to be Mycoplasma-free using EZ-PCR mycoplasma test kit (Biological industries).
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