In order to maintain strains and collect flies for lifespan studies, we used corn meal food (85.7 g corn meal “Aunt Jemina” (The Quaker Oats Company, Chicago, IL), 50 ml golden A unsulfured molasses (Groeb Farms Inc, Onsted, MI), 71.4 g Torula yeast (MP Biomedicals, Solon, OH), 2.86 g p-hydroxybenzoic acid methyl ester (Sigma), 6.4 g agar (MoorAgar Inc, Loomis, CA) and 5.7 ml propionic acid (Sigma) per liter water). In order to test lifespan of flies, fully defined medium was used based on the fruit fly basal mix developed in a recent study [19 (link)] with modification in amino acid content. This diet contained 62.08 g Diet TD.04310 (corresponds to the 0.1X diet) and/or 101.07g Diet TD.10417 (corresponds to the 1.0X diet) (Harlan Teklad), 100 mg lecitin from soybean (Sigma), 500 mg ribonucleic acid from Torula yeast (Sigma), 100 g of dextrose, 20 g agar, 2.85 ml propionic acid, 0.255 ml of phosphoric acid (Sigma), and indicated amounts of Met (Sigma) per liter of water. To prepare 0.4X and 0.7X diets, the two basal mixes were mixed to adjust amino acid concentrations.
Diet td 04310
Manufactured by Inotiv
Diet TD.04310 is a specialized laboratory equipment designed for dietary studies. It provides a controlled and consistent environment for the administration and monitoring of dietary treatments.
Automatically generated - may contain errors
Lab products found in correlation
Torula yeast, by MP Biomedicals (2 mentions)
Phosphoric acid, by Merck Group (2 mentions)
Propionic acid, by Merck Group (2 mentions)
Lecitin from soybean, by Merck Group (2 mentions)
Ribonucleic acid from, by Merck Group (2 mentions)
P hydroxybenzoic acid methyl ester, by Merck Group (2 mentions)
Torula yeast, by Merck Group (2 mentions)
Diet td 10417, by Inotiv (2 mentions)
2 protocols using diet td 04310
1
Drosophila Lifespan Assay Protocols
2
Drosophila Lifespan Assay Protocols
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In order to maintain strains and collect flies for lifespan studies, we used corn meal food (85.7 g corn meal “Aunt Jemina” (The Quaker Oats Company, Chicago, IL), 50 ml golden A unsulfured molasses (Groeb Farms Inc, Onsted, MI), 71.4 g Torula yeast (MP Biomedicals, Solon, OH), 2.86 g p-hydroxybenzoic acid methyl ester (Sigma), 6.4 g agar (MoorAgar Inc, Loomis, CA) and 5.7 ml propionic acid (Sigma) per liter water). In order to test lifespan of flies, fully defined medium was used based on the fruit fly basal mix developed in a recent study [19 (link)] with modification in amino acid content. This diet contained 62.08 g Diet TD.04310 (corresponds to the 0.1X diet) and/or 101.07g Diet TD.10417 (corresponds to the 1.0X diet) (Harlan Teklad), 100 mg lecitin from soybean (Sigma), 500 mg ribonucleic acid from Torula yeast (Sigma), 100 g of dextrose, 20 g agar, 2.85 ml propionic acid, 0.255 ml of phosphoric acid (Sigma), and indicated amounts of Met (Sigma) per liter of water. To prepare 0.4X and 0.7X diets, the two basal mixes were mixed to adjust amino acid concentrations.
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