The chemoselective copper dependent azide-alkyne cyclo addition of pPa was performed in PBS with 250 µM 3[tris(3-hydroxypropyltriazolylmethyl)amine (ThPTA, Sigma-Aldrich, St. Louis MO, U.S.A.), 5 mM sodium ascorbate (Sigma-Aldrich, St. Louis MO, U.S.A.), 50 µM copper sulfate. As reacting agents for pPa, either 10 µM Cy 5-azide (Cy5, Lumiprobe, Hannover, Germany) or 10 mM 10 kDa PEG-azide (PEG10k, Iris Biotech, Marktredwitz, Germany) were used, respectively. The reaction was performed in the dark for 2 h at 25 °C.
Chemoselective Staudinger ligation of AzF was carried out by addition of 10 µM DyLight 650-Phosphine (ThermoFisher Scientific, Waltham MA, U.S.A.) and incubation in the dark for 2 h at 25 °C.
Chemoselective Staudinger ligation of AzF was carried out by addition of 10 µM DyLight 650-Phosphine (ThermoFisher Scientific, Waltham MA, U.S.A.) and incubation in the dark for 2 h at 25 °C.