1 tetradecanol

Manufactured by Merck Group

Sourced in Germany, United States

1-tetradecanol is a long-chain, primary alcohol with the chemical formula CH3(CH2)12CH2OH. It is a white, waxy solid at room temperature. 1-tetradecanol is commonly used as a laboratory reagent and intermediate in the synthesis of various organic compounds.

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Lab products found in correlation

8 protocols using 1 tetradecanol

Polymer-Based Ion-Exchangers Synthesis

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The chemical reagents utilized to cast the PIMs were Poly(vinylidene fluoride-co-hexafluoropropylene) (PVDF-HFP) pellets (Castle Hill, NSW, Australia), di(2-ethylhexyl)phosphoric acid (D2EHPA) (≥ 97%, Sigma-Aldrich, MO, USA), and tetrahydrofuran (THF) (≥ 99.8, Merck, Darmstadt, Germany). The investigated plasticizers were 2-nitrophenyl octyl ether (NPOE) (99%, Sigma Aldrich, Hamburg, Germany), tris(2-ethylhexyl)phosphate (TEHP) (≥ 97%, Sigma Aldrich, Germany), bis(2-ethylhexyl) phthalate (DEHP) (99.5%, Fluka, Buchs, Switzerland), 1-tetradecanol (≥ 98%, Merck, Germany),Tri-n-butyl phosphate (TBP) (> 98%, Merck, Darmstadt, Germany), and phthalic acid dibutyl ester (DBP) (99%, Sigma-Aldrich, USA).
Sulfuric acid (98%, Neutron, Iran), Bi(NO₃)₃ 0.5H₂O (> 99%, Alfa Aesar, ACS), Fe(NO₃)₃·9H₂O (≥ 98%, Alpha Chemika, Maharashtra, India), ZnSO₄·7H₂O (≥ 99%, Sigma-Aldrich, MO, USA), Ni(NO₃)₂ 0.6H₂O (≥ 99%, Carlo Erba, Italy), Cu(NO₃)₂ 0.3H₂O (≥ 99.5%, Avantor, Gliwice, Poland), Na₂SO₄ (≥ 99%, Neutron, Iran), AlCl₃ (≥ 99%), CrCl₃ (99%), Co(NO₃)₃·6H₂O (≥ 98%), Mn(NO₃)₂. 4H₂O (≥ 98%), NaF(≥ 99.5%), NaOH (≥ 98%), and CdSO₄ 0.8H₂O (≥ 98%,), all from Merck (Darmstadt, Germany). All aqueous solutions were prepared in ultrapure water (resistivity ≥ 18.2 MΩ cm, Zolalan, m-uv-3⁺, Iran).

Kazemi D, & Yaftian M.R. (2024). PVDF-HFP-based polymer inclusion membrane functionalized with D2EHPA for the selective extraction of bismuth(III) from sulfate media. Scientific Reports, 14, 11622.

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Synthesis of Magnetic Polymer Sorbents

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All chemicals used for magnetic sorbent synthesis were analytical grade products and were used as received. Glycidyl methacrylate (GMA), ethylene glycol dimethacrylate (EGDMA), diethylenetriamine (deta), 2,2′-Azobis(2-methylpropionitrile) (AIBN), cyclohexanol, and 1-tetradecanol were obtained from Merck (Darmstadt, Germany). Magnetite (nanopowder with particle size 50 nm, ≥98% trace metals basis) and APTMS were obtained from Sigma-Aldrich (Taufkirchen, Germany). Poly(N-vinyl pyrrolidone) (PVP, Kollidone 90) was purchased from BASF (Ludwigshafen, Germany). Toluene, methanol, and ethanol were obtained from Zorka Pharma (Šabac, Serbia). Potassium dichromate (K₂Cr₂O₇, p.a. purity) was obtained from Merck (Darmstadt, Germany). Deionized water was used for the preparation of the solution.

Suručić L., Janjić G., Marković B., Tadić T., Vuković Z., Nastasović A, & Onjia A. (2023). Speciation of Hexavalent Chromium in Aqueous Solutions Using a Magnetic Silica-Coated Amino-Modified Glycidyl Methacrylate Polymer Nanocomposite. Materials, 16(6), 2233.

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Alkane Oxidation in T. oleivorans

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T. oleivorans MIL-1 (DSM 14913) was grown in sterile 160 ml Nunc Cell Culture Treated Flasks containing 100 ml of ONR7a media (Dyksterhouse et al., 1995 (link)). Cultures were enriched separately with the medium-chain alkane n-tetradecane (n-C₁₄), or the long-chain alkane n-octacosane (n-C₂₈). Cultures were incubated in an orbital shaker (16°C, 60 rpm) over a period of 7 days. Each day triplicate cultures of n-C₁₄ and n-C₂₈ were agitated through water sonication for 30 min and pre-concentrated through solid phase extraction (SPE) by Supel-Select HLB cartridges (Supelco, 200 mg, 6 ml) and eluted with 12 ml of chloroform. Samples were further concentrated through evaporation of the chloroform to 200 μl with nitrogen. The concentrated solvent was analyzed to detect the alcohols produced from the oxidation of the alkanes used as growth substrates. GC-MS analysis was carried out as previously described (McKew et al., 2007b (link)). The mass spectrometer scanned m/z values from 10 to 550, with identification of target analytes based on retention times of analytical standards; 1-tetradecanol, 2-tetradecanol and 1-octacosanol (Sigma-Aldrich).

Gregson B.H., Metodieva G., Metodiev M.V., Golyshin P.N, & McKew B.A. (2018). Differential Protein Expression During Growth on Medium Versus Long-Chain Alkanes in the Obligate Marine Hydrocarbon-Degrading Bacterium Thalassolituus oleivorans MIL-1. Frontiers in Microbiology, 9, 3130.

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Proboscis Extension Response Assay

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We collected 3–5-day-old flies in fresh food for 24 hours. Then, we starved them for 18 hours in vials containing 1% agarose. After anaesthetizing the flies on ice, we mounted them on slide glasses with melted 1-tetradecanol (185388, Sigma-Aldrich). We then allowed the flies to recover for 1–2 hours and ensured they were satiated with water before the assay. For each test solution, we used a 1 ml syringe with a 32-gauge needle to apply a single droplet directly to the labellum. We dissolved FAs in 4% ethanol. Each experimental group contained 24 flies, half were mated males and half were mated females, attached to a slide glass. All PER experiments were performed at the same time to eliminate any circadian effects. We report PER responses as the number of responding flies/total flies.

Kim H., Kim H., Kwon J.Y., Seo J.T., Shin D.M, & Moon S.J. (2018). Drosophila Gr64e mediates fatty acid sensing via the phospholipase C pathway. PLoS Genetics, 14(2), e1007229.

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Supramolecular Extraction of Aluminum

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Analytical grade chemicals including 1-hexanol (≥ 99%, d = 0.814 g mL⁻¹), 1-dodecanol (≥ 98.0%, d = 0.83 g cm⁻³), 1-decanol (≥ 99.0%, d = 0.80 g cm⁻³), 1-tetradecanol (≥ 97%, d = 0.823 g cm⁻¹, Sigma), and tetrahydrofuran (Carlo Erba, THF) were used to prepare alkanol-based SUPRASs. Ultrapure water was prepared by Milli-Q water purification system (Millipore, Bedford, MA, USA). Stock solution (1000 mg L⁻¹) of Al(III) ion was prepared from Al(NO₃)₃ salt (Sigma, St Louis, MO, USA) in water and stored at 4 °C in a refrigerator. A stock solution of morinhydrate was prepared in ethanol at a concentration of 1000 mg L⁻¹ (Carlo Erba, Milan, Italy) and stored in dark. The calibration solutions were freshly prepared by sequential dilution of the stock solutions. A 5% (w/v) of KCl solution was prepared by dissolving 5.0 g of KCl (Sigma) in 100 mL deionized water. Borate, acetate, phthalate, and citrate buffer solutions were used to adjust solutions pH. To minimize all possible contamination of glassware, a diluted HNO₃ solution was used for washing glassware and finally rinsed with distilled water.

Demir Ö., Tuzen M., Altunay N, & Mogaddam M.R. (2021). Air-Assisted Alkanol-Based Nanostructured Supramolecular Liquid–Liquid Microextraction for Extraction and Spectrophotometric Determination of Morin in Fruit and Beverage Samples. Food Analytical Methods, 15(1), 243-251.

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Synthesis of Magnetic Nanoparticles for Cell Studies

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FeCl₂ · 4H₂O, FeCl₃ · 6H₂O, 1-tetradecanol (TD), Dulbecco's modified Eagle's culture medium (DMEM), Hoechst 33342, and propidium iodide (PI) were purchased from Sigma-Aldrich (St. Louis, MO, USA); 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) was from Biomedica (Vienna, Austria). Twenty five percent NH₄OH, 70% oleic acid (OA), dichloromethane (DCM), and methylene chloride were obtained from Lach-Ner (Neratovice, Czech Republic). Trypan blue DV-T10282 was from Life Technologies (Carlsbad, CA, USA). Doxorubicin (Dox) was purchased from Actavis (Bucharest, Romania), vincristine was from Teva (Haarlem, Netherlands), Roswell Park Memorial Institute (RPMI) culture medium and fetal bovine serum (FBS) were from APP (Vienna, Austria). All other chemicals were purchased from Sigma-Aldrich. Poly(ethylene oxide)-block-poly(ε-caprolactone) (BL; M_n = 6,500 Da, M_w = 7,100 Da, polydispersity = 1.1) was synthesized by a ring-opening polymerization of ε-caprolactone initiated by poly(ethylene oxide) monomethyl ether (degree of polymerization = 45), as previously described (Trousil et al., 2019 (link)). Ultrapure water for the magnetic particle synthesis was obtained from a Milli-Q Gradient A10 system (Millipore; Molsheim, France).

Świętek M., Panchuk R., Skorokhyd N., Černoch P., Finiuk N., Klyuchivska O., Hrubý M., Molčan M., Berger W., Trousil J., Stoika R, & Horák D. (2020). Magnetic Temperature-Sensitive Solid-Lipid Particles for Targeting and Killing Tumor Cells. Frontiers in Chemistry, 8, 205.

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Electrospun Polycaprolactone Nanofibers with Solid Lipid Particles

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Polycaprolactone (Mw = 45,000, Sigma Aldrich, Saint Luis, MO, USA) was used for the nanofiber substrates and acetic acid (99% pure, Penta Chemicals, Prague, Czech Republic), chloroform (VWR Chemicals, Leuven, Belgium), ethanol (96%, P-lab, Prague, Czech Republic), and acetone (AC) (P-lab/Carl Roth GmbH, Karlsruhe, Germany) were used as the solvents. Absolute ethanol (Penta Chemicals, Prague, Czech Republic) was used for washing the fibers after their preparation and for disinfection. The solid lipid particles (SLP) were prepared from 1-tetradecanol (Sigma Aldrich, Steinheim, Germany), distilled water, Tween 20 (Molecular biology grade, WVR Chemicals, Solon, OH, USA), and ethanol (absolute, Penta Chemicals in, Prague, Czech Republic) an antioxidant SLP preparation. Gentamicin sulphate (BioChemica, Darmstadt, Germany) was used as an antibacterial substance in a solution in distilled water. α-tocopherol was used as the antioxidant substance (from vegetable oil, type V, Sigma Aldrich, Saint Luis, MO, USA).

Braťka P., Fenclová T., Hlinková J., Uherková L., Šebová E., Hefka Blahnová V., Hedvičáková V., Žižková R., Litvinec A., Trč T., Rosina J, & Filová E. (2022). The Preparation and Biological Testing of Novel Wound Dressings with an Encapsulated Antibacterial and Antioxidant Substance. Nanomaterials, 12(21), 3824.

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Solubilization of APIs in Biorelevant Media

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Three active pharmaceutical ingredients (APIs) were ibuprofen (IBU: 99%, Alfa Aesar, Tewksbury, MA, USA), naproxen (NPX: ≥ 98%, Tokyo Chemical Industry, Tokyo, Japan), and sorafenib (SOR: ≥ 99%, Jinan Rouse Industry, Jinan, China). All fatty alcohols were purchased from Sigma-Aldrich (St. Louis, MO, USA): 1-tetradecanol (TD, CH₃(CH₂)₁₃OH: ≥ 95%), 1-octadecanol (OD, CH₃(CH₂)₁₇OH: 99%), and 1-docosanol (DC, CH₃(CH₂)₂₁OH: 98%).
To make a fed state simulated intestinal fluid (FeSSIF), FaSSIF/FeSSIF/FaSSGF powder was purchased from Biorelevant (London, UK). Sodium chloride (NaCl, ≥ 99.5%), sodium hydroxide (NaOH, ≥ 97%), and acetic acid (≥ 99%) were from Sigma-Aldrich. Further, 1 M HCl (aq) and 1 M NaOH (aq) were obtained from Samchun Chemical (Seoul, Korea) and Daejung Chemical (Gyeonggi, Korea), respectively. Deionized water (DI water, resistivity > 18.2 MΩ·cm) was from a Direct-Q3 water purification system (Millipore, Burlington, MA, USA).

Jin S., Jang J., Lee S, & Kim I.W. (2020). Binary Mixtures of Some Active Pharmaceutical Ingredients with Fatty Alcohols—The Criteria of Successful Eutectic Formation and Dissolution Improvement. Pharmaceutics, 12(11), 1098.

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