60 f254 silica

Manufactured by Merck Group

60 F254 silica is a type of thin-layer chromatography (TLC) plate used for analytical and preparative separations. It consists of a thin layer of silica gel with a fluorescent indicator F254 coated on a rigid support, typically glass or aluminum. The silica gel serves as the stationary phase, while the mobile phase is a solvent or solvent mixture. The fluorescent indicator allows for easy visualization of separated components under UV light.

Automatically generated - may contain errors

Lab products found in correlation

Graceresolve, by Agilent Technologies (2 mentions)

Close spelling variants of this product

TLC Silica gel 60 RP-18 F254S Silica gel 60 RP-18 F254S plate TLC Silica Gel 60 W F254s Silica 60 RP-18 F254S aluminum sheet Silica gel 60 RP-18 F254S pre-coated plates Silica gel 60 RP-18 F254S Silica gel 60 F254 precoated glass plates Silica gel 60 F254 aluminum plates TLC Silica gel 60 F254 aluminium Merck pre-coated silica gel 60 F254 plates

2 protocols using 60 f254 silica

Automated Purification and Characterization

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Chemicals were purchased from commercial
suppliers and used without further purification. Thin layer chromatography
(TLC) was performed on aluminum plates coated with 60 F₂₅₄ silica from Merck. Flash chromatography was carried out using a
Biotage SP4, Biotage Isolera, or Varian automated flash system with
Silicycle or GraceResolve normal phase silica gel prepacked columns.
Fractions were collected at 254 nm or if necessary on all wavelengths
between 200 and 400 nm. Microwave irradiation was performed in a Biotage
Initiator Sixty in sealed vials. Reactions were irradiated at 2.45
GHz and were able to reach temperatures between 60 and 250 °C.
Heating was at a rate of 2–5 °C/s, and the pressure was
able to reach 20 bar. Final compound purity is >95%.

Uguen M., Davison G., Sprenger L.J., Hunter J.H., Martin M.P., Turberville S., Watt J.E., Golding B.T., Noble M.E., Stewart H.L, & Waring M.J. (2022). Build–Couple–Transform: A Paradigm for Lead-like Library Synthesis with Scaffold Diversity. Journal of Medicinal Chemistry, 65(16), 11322-11339.

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Purification and Characterization of Organic Compounds

Check if the same lab product or an alternative is used in the 5 most similar protocols

Chemicals were purchased from commercial
suppliers and used without further purification. Thin-layer chromatography
(TLC) was performed on aluminum plates coated with 60 F₂₅₄ silica from Merck. Flash chromatography was carried out using a
Biotage SP4, Biotage Isolera, or Varian automated flash system with
Silicycle or GraceResolve normal-phase silica gel pre-packed columns.
Fractions were collected at 254 nm or, if necessary, on all wavelengths
between 200 and 400 nm. Microwave irradiation was performed in a Biotage
Initiator Sixty in sealed vials. Reactions were irradiated at 2.45
GHz and were able to reach temperatures between 60 and 250 °C.
Heating was at a rate of 2–5 °C/s, and the pressure was
able to reach 20 bar. Final compound purity is >95% determined
by
HPLC or NMR.

Davison G., Martin M.P., Turberville S., Dormen S., Heath R., Heptinstall A.B., Lawson M., Miller D.C., Ng Y.M., Sanderson J.N., Hope I., Wood D.J., Cano C., Endicott J.A., Hardcastle I.R., Noble M.E, & Waring M.J. (2022). Mapping Ligand Interactions of Bromodomains BRD4 and ATAD2 with FragLites and PepLites—Halogenated Probes of Druglike and Peptide-like Molecular Interactions. Journal of Medicinal Chemistry, 65(22), 15416-15432.

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