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Nanofil 100

Manufactured by World Precision Instruments
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The NANOFIL-100 is a precision laboratory instrument designed for microfluidic applications. It features a high-resolution syringe pump capable of delivering nanoliter volumes with exceptional accuracy and repeatability. The core function of the NANOFIL-100 is to provide precise fluid control for researchers and scientists working in the field of microfluidics.

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Nanofil syringe, by World Precision Instruments (1 mentions)

Close spelling variants of this product

NanoFil (30 citations) NanoFil-100 syringe (3 citations) NANOFIL-100 injector system (3 citations) Nanofil 100 μl; 33G beveled needle (2 citations)

3 protocols using nanofil 100

1

Fetal Vascular Labeling via Intracardiac Lectin Injection

Cited 1 time
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Prior to fetal harvest, we performed an in utero intracardiac injection of fluorescein-conjugated tomato-lectin (TL) for CDH fetuses in utero as previously described (Shah et al., 2011 (link)). An ultrasound microscope probe was used to guide the injection apparatus. Sub-microliter injection system (Nanofil-100, World Precision Instruments, Sarasota, FL, United States of America) was used to inject TL into the heart. Each fetal heart was injected with 40 μL of TL. The injected fluorescent-conjugated lectin was allowed to circulate for 10 min to ensure adequate binding to the vascular endothelial wall of the vasculature, after which the fetus was harvested and fixed in 4% PFA for immunofluorescent analyses.
Yoshida S., Kreger A.M, & Gittes G.K. (2023). Intra-amniotic sildenafil treatment improves lung blood flow and pulmonary hypertension in congenital diaphragmatic hernia rats. Frontiers in Bioengineering and Biotechnology, 11, 1195623.
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2

Quantifying Cell Proliferation in Zebrafish

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To analyse cell proliferation, 5-ethynyl-2′-deoxyuridine (EdU)-Click 647 kit (baseclick GmbH BCK-EdU647) was used. Fish were IP injected with 10 µl of 10 mM EdU in PBS using a 100 µl Nanofil syringe (World Precision Instruments NANOFIL-100). In amputation assays, fish were injected as indicated in the figures. In the hemiray removal assay, fish were injected once at 1 dpi for evaluation at 2 dpi, and at 1 and 2 dpi for evaluation at 3 dpi. Fins were fixed with 4% PFA overnight and EdU labelling was performed according to the manufacturer’s protocol. If applicable, EdU labelling was followed with AB staining following the immunohistochemistry protocol, or RNAscope in situ following the hybridisation protocol.
Sehring I., Mohammadi H.F., Haffner-Luntzer M., Ignatius A., Huber-Lang M, & Weidinger G. (2022). Zebrafish fin regeneration involves generic and regeneration-specific osteoblast injury responses. eLife, 11, e77614.
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3

Tracing SP-IR Axons in Mouse Stomach

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To verify whether the SP-IR axons in the stomach originated from the dorsal root ganglia (DRG) and vagal nodose-petrosal ganglia complex (VNG), mice (n=5) were anesthetized with isoflurane (2–3%). The depth of anesthesia was determined by absence of the hind paw pinch withdrawal reflex. Tracer Fast DiI (Invitrogen, Catalog# D3899) was injected into the ventral or dorsal stomach walls using a nanofil syringe microinjection system (World Precision Instrument, NANOFIL-100) in the regions of the fundus, corpus, and antrum-pylorus to cover the whole surface of the ventral or dorsal walls. Each region received 3–4 injections (2 μl/each, 9–12 injections per surface). Following injections, the incisions were closed and the animals were returned to their cages. 14–16 days after injection, animals were perfused, and the left and right DRGs (T1–12), as well as the left and right VNG, were removed for IHC labeling of SP.
Blake J.A., Richardson J.E., Bult C.J., Kadin J.A, & Eppig J.T. (2002). The Mouse Genome Database (MGD): the model organism database for the laboratory mouse. Nucleic Acids Research, 30(1), 113-115.
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