3d discovery gen 1

Printing tests were performed with a bioplotter (3D Discovery Gen 1, RegenHU Ltd., Villaz-St-Pierre, Switzerland). Cannulas with a diameter of 0.25 and 0.41 mm and a pressure up to 4.5 bar were used for printing. For better adhesion of the printed constructs, the covers of microtiter plates were coated with a HA solution. The number of printed layers were 8 and 12. Gels with a final composition of 3.5% (w/w) HMW-AHA-2.0 with 0.05, 0.075 and 1.0% (w/w) ADH were used for printing.

A regenHU 3D Discovery Gen1 (Villaz-Saint-Pierre, Switzerland) bioplotter was used for bioprinting, equipped with cartridges size 3cc and regenHU conical pistons. Luer lock conical needles were adapted to the cartridges with an inner diameter of 14 G, 16 G or 20 G. The different materials were filled into the cartridges manually using block volumes of 0.5–1 mL. Polystyrene Petri dishes were used as substrate (diameter 8 cm, Sarstedt, Nümbrecht, Germany). A round-shaped monolayer construct was designed, and the according G-code was generated using the regenHU BioCAD V1.1 printer software. The applied pressure was set to 0.1 bar for all hydrogels. The printing speed was pre-set to 20 mm/s.