Dmem ha

The cell culture was performed as described [1 (link)]. Briefly, C2C12 mouse progenitor myoblast cells (RRID:CVCL_0188) were seeded on 10 and 18 mm laminin-coated coverslips in DMEM/High Glucose (Capricorn Scientific DMEM-HA) with 1% penicillin and streptomycin (Capricorn Scientific PS-B) containing 15% fetal bovine serum (Gibco 10100147) until confluent (approx. 100,000 cells/cm²). Differentiation was induced by switching to a medium containing 2% horse serum (Gibco 26050088) for 6 days.

U87 and U251 glioblastoma cell lines (identity of cell lines was verified by karyotyping and STR profiling) were obtained from ECACC and were cultured in DMEM cell medium (DMEM-HA, Capricorn Scientific, Ebsdorfergrund, Germany) supplemented with 10% fetal bovine serum (FBS) (S0615, Sigma, Dreieich, Germany), 1% penicillin/streptomycin (2321115, Gibco, Carlsbad, CA, USA), 1% Non-essential amino acids (NEAA) (11140050, Gibco, Carlsbad, USA) and 1% Sodium pyruvate (NPY-B, Capricorn Scientific, Ebsdorfergrund, Germany). Glioblastoma stem-like cells were isolated as described previously [12 (link)]. Cells were cultured in DMEM/F12 medium (DMEM-12-A, Capricorn Scientific, Ebsdorfergrund, Germany) including 2% B27 supplement (117504044, Gibco, Carlsbad, USA), 1% amphotericin (152290026, Gibco, Carlsbad, USA), 0.5% HEPES (H0887, Sigma, Dreieich, Germany) and 0.1% gentamycin (A2712, Biochrom, Berlin, Germany) with EGF (100-18B, Peprotech, Hamburg, Germany) and bFGF (315-09, Peprotech, Hamburg, Germany) in a concentration of 20 ng/mL, respectively. All cells were incubated at 37 °C under 5% CO₂ humidified incubator.