Lanthanide chlorides (YCl3·6H2O, YbCl3·6H2O and ErCl3·6H2O (>99.9%, all of them)), 1-octadecene (95%), oleic acid (99.9%), NaOH, NH4F (99.99%), and cucurbit[7]uril were purchased from Sigma-Aldrich (Madrid, Spain) and used as received without previous purification.
Cucurbit 7 uril
Manufactured by Merck Group
Sourced in Spain, United Kingdom
Cucurbit[7]uril is a macrocyclic compound composed of seven glycoluril units linked by methylene bridges. It is a member of the cucurbit[n]uril family of host-guest molecules, which are known for their ability to form stable complexes with a variety of guest molecules. Cucurbit[7]uril exhibits a barrel-shaped structure with a hydrophobic cavity and polar carbonyl-lined portals, making it useful as a molecular host for various applications.
Automatically generated - may contain errors
Lab products found in correlation
Lanthanide chlorides, by Merck Group (1 mentions)
Oleic acid, by Merck Group (1 mentions)
1 octadecene, by Merck Group (1 mentions)
Sodium hydroxide (naoh), by Merck Group (1 mentions)
Methyl viologen, by Merck Group (1 mentions)
β trypsinfrom bovine pancreas, by Merck Group (1 mentions)
Coumarin 343, by Merck Group (1 mentions)
Methylene violet, by Merck Group (1 mentions)
Citric acid sodium citrate, by Merck Group (1 mentions)
6 amino 2 naphthoic acid, by Merck Group (1 mentions)
Biphenyl 4 carboxylic acid, by Merck Group (1 mentions)
4 aminobenzoic acid, by Merck Group (1 mentions)
β cyclodextrin, by Merck Group (1 mentions)
3 protocols using cucurbit 7 uril
1
Lanthanide-Based Nanoparticle Synthesis
2
Synthesis and Characterization of Multifunctional Molecular Assemblies
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Cucurbit[8]uril, cucurbit[7]uril, β-trypsin
from bovine pancreas, and methyl viologen were purchased from Sigma-Aldrich.
The disulfides (bis-1-(11-{tetraethylene glycol}-undecyl) disulfide
(EG4C11S)2 and N-{2-(2,5-dioxo-2,5-dihydro-pyrrol-1-yl)-ethyl}-[2-[11-(11-(tetraethylene
glycol)-undecyldisulfanyl)-undecyloxy]-hexaethylene glycol-acetamide])
Mal-EG6C11SSC11EG4) for
SAM preparation were purchased from ProChimia. Alkyl thiol terminated
MV2+ (MV2+-SH) was synthesized as previously
reported.44 (link) NpGGRGDSG (Np-RGD) was synthesized
using an automatic solid-phase synthetic robot (Syro II, Multisyntech)
following standard Fmoc procedures on RinkAmide resin. GGCGGRGDS (C-RGD)
was synthesized with a MultiPep RSi, Intavis Bioanalytical Instruments
using standard solid-phase peptide synthesis protocols on a Wang resin.
Purification was done by reversed-phase high-performance liquid chromatography
(HPLC (Waters)), followed by analysis with analytical HPLC and mass
spectrometry. Because of the poor solubility of CB[8] in water and
its hygroscopic nature, the apparent molecular weight of the commercial
powder and its actual concentration in aqueous solutions were determined
for each batch using a simple and highly reproducible method described
previously.45 (link) CB[8] was dissolved in Milli-Q
water by sonication at 80 °C for 2 h.
from bovine pancreas, and methyl viologen were purchased from Sigma-Aldrich.
The disulfides (bis-1-(11-{tetraethylene glycol}-undecyl) disulfide
(EG4C11S)2 and N-{2-(2,5-dioxo-2,5-dihydro-pyrrol-1-yl)-ethyl}-[2-[11-(11-(tetraethylene
glycol)-undecyldisulfanyl)-undecyloxy]-hexaethylene glycol-acetamide])
Mal-EG6C11SSC11EG4) for
SAM preparation were purchased from ProChimia. Alkyl thiol terminated
MV2+ (MV2+-SH) was synthesized as previously
reported.44 (link) NpGGRGDSG (Np-RGD) was synthesized
using an automatic solid-phase synthetic robot (Syro II, Multisyntech)
following standard Fmoc procedures on RinkAmide resin. GGCGGRGDS (C-RGD)
was synthesized with a MultiPep RSi, Intavis Bioanalytical Instruments
using standard solid-phase peptide synthesis protocols on a Wang resin.
Purification was done by reversed-phase high-performance liquid chromatography
(HPLC (Waters)), followed by analysis with analytical HPLC and mass
spectrometry. Because of the poor solubility of CB[8] in water and
its hygroscopic nature, the apparent molecular weight of the commercial
powder and its actual concentration in aqueous solutions were determined
for each batch using a simple and highly reproducible method described
previously.45 (link) CB[8] was dissolved in Milli-Q
water by sonication at 80 °C for 2 h.
3
Characterization of Fluorescent Probes
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6-amino-2-naphthoic acid (ANA), 4-aminobenzoic acid (ABA), 4′-amino-[1,1′-biphenyl]-4-carboxylic acid (ABPA), methylene violet (Bernthsen) (MV), coumarin 343 (C343), β-cyclodextrin (β-CD), cucurbit[7]uril (CB7), citric acid/sodium citrate, sodium dihydrogen phosphate/disodium hydrogen phosphate, boric acid/sodium borate and sodium N-cyclohexyl-3-aminopropanesulfonate (CAPS) were obtained from Sigma Aldrich UK and were of the highest purity available.
5, 6-diamino-naphthalene-1, 3-disulfonic acid (DANDSA) was obtained from Molekula, UK.
4-pyridinium phenolate (4PP) was synthesised and provided by WuXi App Tec (Hong Kong) Ltd. Structure and purity were confirmed by NMR.
The provenances of Brooker's merocyanine (BM), bis-dimethylaminofuchsone (BDF), phenol blue (PB), and 2, 6-dichloro-4-(2, 4, 6-triphenyl-pyridinium-1-yl)-phenolate (ET33) are as stated previously.
1 (link)
2 (link)
ROW was used throughout this study and had a resistivity of 15MΩcm (checked daily).
Disposable high purity UV-transparent cuvettes (Brand GmbH) with stoppers were used throughout and are described previously.
1 (link)
Disposable four-sided optically transparent fluorescence cuvettes (Brand GmbH) made of the same material as the UV cuvettes, with stoppers, were used to record fluorescence spectra.
5, 6-diamino-naphthalene-1, 3-disulfonic acid (DANDSA) was obtained from Molekula, UK.
4-pyridinium phenolate (4PP) was synthesised and provided by WuXi App Tec (Hong Kong) Ltd. Structure and purity were confirmed by NMR.
The provenances of Brooker's merocyanine (BM), bis-dimethylaminofuchsone (BDF), phenol blue (PB), and 2, 6-dichloro-4-(2, 4, 6-triphenyl-pyridinium-1-yl)-phenolate (ET33) are as stated previously.
1 (link)
2 (link)
ROW was used throughout this study and had a resistivity of 15MΩcm (checked daily).
Disposable high purity UV-transparent cuvettes (Brand GmbH) with stoppers were used throughout and are described previously.
1 (link)
Disposable four-sided optically transparent fluorescence cuvettes (Brand GmbH) made of the same material as the UV cuvettes, with stoppers, were used to record fluorescence spectra.
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