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Cucurbit 7 uril

Manufactured by Merck Group
Sourced in Spain, United Kingdom

Cucurbit[7]uril is a macrocyclic compound composed of seven glycoluril units linked by methylene bridges. It is a member of the cucurbit[n]uril family of host-guest molecules, which are known for their ability to form stable complexes with a variety of guest molecules. Cucurbit[7]uril exhibits a barrel-shaped structure with a hydrophobic cavity and polar carbonyl-lined portals, making it useful as a molecular host for various applications.

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3 protocols using cucurbit 7 uril

1

Lanthanide-Based Nanoparticle Synthesis

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Lanthanide chlorides (YCl3·6H2O, YbCl3·6H2O and ErCl3·6H2O (>99.9%, all of them)), 1-octadecene (95%), oleic acid (99.9%), NaOH, NH4F (99.99%), and cucurbit[7]uril were purchased from Sigma-Aldrich (Madrid, Spain) and used as received without previous purification.
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2

Synthesis and Characterization of Multifunctional Molecular Assemblies

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Cucurbit[8]uril, cucurbit[7]uril, β-trypsin
from bovine pancreas, and methyl viologen were purchased from Sigma-Aldrich.
The disulfides (bis-1-(11-{tetraethylene glycol}-undecyl) disulfide
(EG4C11S)2 and N-{2-(2,5-dioxo-2,5-dihydro-pyrrol-1-yl)-ethyl}-[2-[11-(11-(tetraethylene
glycol)-undecyldisulfanyl)-undecyloxy]-hexaethylene glycol-acetamide])
Mal-EG6C11SSC11EG4) for
SAM preparation were purchased from ProChimia. Alkyl thiol terminated
MV2+ (MV2+-SH) was synthesized as previously
reported.44 (link) NpGGRGDSG (Np-RGD) was synthesized
using an automatic solid-phase synthetic robot (Syro II, Multisyntech)
following standard Fmoc procedures on RinkAmide resin. GGCGGRGDS (C-RGD)
was synthesized with a MultiPep RSi, Intavis Bioanalytical Instruments
using standard solid-phase peptide synthesis protocols on a Wang resin.
Purification was done by reversed-phase high-performance liquid chromatography
(HPLC (Waters)), followed by analysis with analytical HPLC and mass
spectrometry. Because of the poor solubility of CB[8] in water and
its hygroscopic nature, the apparent molecular weight of the commercial
powder and its actual concentration in aqueous solutions were determined
for each batch using a simple and highly reproducible method described
previously.45 (link) CB[8] was dissolved in Milli-Q
water by sonication at 80 °C for 2 h.
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3

Characterization of Fluorescent Probes

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6-amino-2-naphthoic acid (ANA), 4-aminobenzoic acid (ABA), 4′-amino-[1,1′-biphenyl]-4-carboxylic acid (ABPA), methylene violet (Bernthsen) (MV), coumarin 343 (C343), β-cyclodextrin (β-CD), cucurbit[7]uril (CB7), citric acid/sodium citrate, sodium dihydrogen phosphate/disodium hydrogen phosphate, boric acid/sodium borate and sodium N-cyclohexyl-3-aminopropanesulfonate (CAPS) were obtained from Sigma Aldrich UK and were of the highest purity available.
5, 6-diamino-naphthalene-1, 3-disulfonic acid (DANDSA) was obtained from Molekula, UK.
4-pyridinium phenolate (4PP) was synthesised and provided by WuXi App Tec (Hong Kong) Ltd. Structure and purity were confirmed by NMR.
The provenances of Brooker's merocyanine (BM), bis-dimethylaminofuchsone (BDF), phenol blue (PB), and 2, 6-dichloro-4-(2, 4, 6-triphenyl-pyridinium-1-yl)-phenolate (ET33) are as stated previously.
1 (link)
2 (link)
ROW was used throughout this study and had a resistivity of 15MΩcm (checked daily).
Disposable high purity UV-transparent cuvettes (Brand GmbH) with stoppers were used throughout and are described previously.
1 (link)
Disposable four-sided optically transparent fluorescence cuvettes (Brand GmbH) made of the same material as the UV cuvettes, with stoppers, were used to record fluorescence spectra.
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