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Coumarin 6 dye

Manufactured by Merck Group
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Coumarin 6 dye is a fluorescent dye used in various laboratory applications. It has an excitation wavelength of approximately 450 nm and an emission wavelength of around 500 nm. Coumarin 6 dye is commonly used as a fluorescent tracer or marker in analytical and imaging techniques.

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Lab products found in correlation

Acetonitrile, by Thermo Fisher Scientific (2 mentions) Polyvinyl alcohol (pva), by Merck Group (2 mentions) Dimethyl sulfoxide (dmso), by Merck Group (1 mentions) Polyvinyl alcohol (pva), by Thermo Fisher Scientific (1 mentions) Dichloromethane, by Thermo Fisher Scientific (1 mentions) Polyvinyl chloride, by Thermo Fisher Scientific (1 mentions) Bovine serum albumin (bsa), by Merck Group (1 mentions) Chloroform, by Thermo Fisher Scientific (1 mentions) Dexamethasone dex, by Enzo Life Sciences (1 mentions) Trizol reagent, by Thermo Fisher Scientific (1 mentions) Dialysis bag, by Serva Electrophoresis (1 mentions) Dimethyl sulfoxide (dmso), by Thermo Fisher Scientific (1 mentions) 3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt, by Serva Electrophoresis (1 mentions) Confocal microscope, by Zeiss (1 mentions) Draq5, by Thermo Fisher Scientific (1 mentions)

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Coumarin-6 (218 citations) Coumarin (169 citations)

5 protocols using coumarin 6 dye

1

Formulation and Characterization of PEG-PLGA Nanoparticles

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Poly (ethylene glycol)-methyl ether-block -poly (lactide-co-glycolide) (PEG-PLGA50:50, MnPEG 2000 Da and 10,000 Da MnPLGA), dimethyl sulfoxide (DMSO), and coumarin 6 dye were purchased from Sigma Aldrich (St. Louis, MO, USA). Rhodamine 6G dye, dichloromethane (DCM), polyvinyl alcohol (PVA) (87–89% hydrolyzed, high molecular weight), acetonitrile, phosphate buffer saline (PBS), polyvinyl chloride (PVC) tubing, syringes (glass and plastic), syringe needles, and polytetrafluoroethylenes (PTFE) based consumables such as syringe filters (0.45 μm), evaporating dish, and magnetic stirring bars were obtained from Fisher Scientific. All other chemicals and reagents used in this study were of analytical grade.
Sagoe P.N., Velázquez E.J., Espiritusanto Y.M., Gilbert A., Orado T., Wang Q, & Jain E. (2023). Fabrication of PEG-PLGA Microparticles with Tunable Sizes for Controlled Drug Release Application. Molecules, 28(18), 6679.
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2

Polymeric Nanoparticle Formulation

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For formulation of polymeric NPs, we used PLGA (molecular weight 45,000–75,000), copolymer ratio 50:50, polyvinyl alcohol (PVA; average molecular weight 30,000–70,000), bovine serum albumin (BSA), Coumarin 6 dye, dimethyl tartaric acid (DMT), dichloromethane (DCM), and Ps80 (Tween 80), all purchased from Sigma-Aldrich (St Louis, MO, USA).
Chaturvedi M., Molino Y., Sreedhar B., Khrestchatisky M, & Kaczmarek L. (2014). Tissue inhibitor of matrix metalloproteinases-1 loaded poly(lactic-co-glycolic acid) nanoparticles for delivery across the blood–brain barrier. International Journal of Nanomedicine, 9, 575-588.
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3

Polymer Blend Nanoparticle Formulation

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Carboxyl-terminated PS-b-PEO (molecular weight 9500-b-18000 Da) (Cat No P5755-SEOCOOH) was purchased from Polymer Source Inc. (Montreal, QC, Canada). Poly(dl-lactide-co-glycolide) (molecular weight 50–70 kDa, Cat No B6010-3) was purchased from Lactel Absorbable Polymers. Poly(vinyl alcohol) (PVA; 13,000–23,000 g/gmol) (Cat No 363170) was purchased from Sigma-Aldrich, and chloroform (Cat No C606SK-4) was purchased from Thermo Fisher Scientific. Dexamethasone (DEX; Cat No BML-EI126-0001) was purchased from Enzo Life Sciences. Coumarin 6 dye was purchased from Sigma-Aldrich (Cat No 442631). All chemicals were used as received without further purification.
Nabar G.M., Mahajan K.D., Calhoun M.A., Duong A.D., Souva M.S., Xu J., Czeisler C., Puduvalli V.K., Otero J.J., Wyslouzil B.E, & Winter J.O. (2018). Micelle-templated, poly(lactic-co-glycolic acid) nanoparticles for hydrophobic drug delivery. International Journal of Nanomedicine, 13, 351-366.
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4

Resveratrol and Thymol Formulation Evaluation

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Resveratrol (RSV) (high purity > 98%) was purchased from Guangzhou Phytochem Sciences Inc. (Guangzhou, China). Thymol was purchased from Oxford Lab Fine Chem I.I.P (India). Dialysis bags (molecular weight cut off 12,000-14,000 Da) (Serva, Heidelberg, Germany). Coumarin-6 dye was obtained from Sigma-Aldrich (St. Louis, MO, USA). 3-(4,5-dimethylthiazol-2-yl)-2,5diphenyltetrazolium bromide (MTT) was supplied by SERVA Electrophoresis GmbH (Germany). TRIzol ® Reagent was provided by Invitrogen, Thermo Fisher Scientific (Waltham, MA, USA). The primers were acquired from Sigma-Aldrich (UK) and a one-step RT qPCR kit (SYBR Green with low ROX) was purchased from Intron Biotechnology, Korea. B-cell Lymphoma/Leukemia 2 ELISA kit was purchased from Novus Biologicals, UK (NBP2-69946), and the Nuclear Factor Kappa B ELISA kit was obtained from Wuhan Fine Biotech Co., Ltd (Wuhan, Hubei, China). Acetonitrile and DMSO (HPLC grade) were purchased from Fischer Scientific (Loughborough, UK). Methanol, chloroform, ethanol, and formalin were provided by Al-Gomhureya Co. (Cairo, Egypt). All other reagents and chemicals were of analytical grade.
Samir B., El-Kamel A., Zahran N, & Heikal L. (2024). Resveratrol-loaded invasome gel: A promising nanoformulation for treatment of skin cancer. Drug delivery and translational research.
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5

Visualizing Nanoparticle-Cancer Cell Interactions

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Nanoparticle interactions with cancer cells were observed using confocal microscopy. HCT116 colon cancer cells and PC3 prostate cancer cells were seeded at a density of 3 × 105 cells/well in a four-well tissue culture slide (BD Biosciences, Bedford, MA). The cells were incubated for 24 hours at 37°C. Cells were treated with 625 ng of PLGA nanoparticles containing Coumarin 6 dye (Sigma Aldrich, St. Louis, MO) and were incubated for an additional 2, 4, or 24 hours. Cells were stained with DRAQ5 (Life Technologies, Grand Island, NY) nuclear staining dye after incubation with the particles. Nanoparticle interactions were observed using a Zeiss Confocal Microscope (Zeiss, Peabody, MA).
Sharkey C.C., Li J., Roy S., Wu Q, & King M.R. (2016). Two-stage nanoparticle delivery of piperlongumine and tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) anti-cancer therapy. Technology, 4(1), 60-69.
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