Zd7288 hydrate

DL-APV was purchased from R&D Systems (Minneapolis, MN, USA). Tetrodotoxin (cat# 5651), TEA (cat# 2265), 4-AP (cat# A78403), DNQX (cat# D0540), SR-95531 (cat# S106), and ZD7288 hydrate (cat# Z3777) were purchased from Sigma-Aldrich, Inc. (Saint Louis, MO, USA). Salts and chemicals for saline solutions, including biocytin, were also purchased from Sigma-Aldrich, Inc.

Ivabradine hydrochloride, ZD7288 hydrate, NA, histamine, pyrilamine maleate, CsCl, and BSA were purchased from Sigma‐Aldrich. Stock solutions were made in deionized water and stored in aliquots at −20°C until use. PSS had the following composition (in mM): NaCl 119; CaCl₂ 1.6; KCl 4.7; MgSO₄ 1.17; NaHCO₃ 25; KH₂PO₄ 1.18; EDTA 0.026; glucose 5.5. In 125 mM K⁺ solution (KPSS) all NaCl is substituted with KCl, while the 50 mM K⁺ solution was made by mixing appropriate volumes of PSS and KPSS. For immunofluorescence, sterile solutions of phosphate‐buffered saline (PBS) (in mM) 138 NaCl, 2.67 KCl, 8.1 Na₂HPO₄, 1.47 KH₂PO₄, and TEG‐buffer (pH 9) composed of 10 mM tris base and 5 mM EGTA were used.