The reference strain Pseudomonas aeruginosa PA14 (DSM 50071) and two mutant strains with a transposon insertion within the pqsA::Tn or the pelA::Tn gene were applied in this study10 (link), 17 (link), 71 (link). Both mutants have been shown to result in a low biofilm morphotype16 (link), 18 . The mutant strains were obtained from the PA14 transposon mutant library of the F.M. Ausubel lab. And the strains were stored in glycerol stocks at −70 °C. The pre-cultures (4 ml lysogeny broth (LB) medium) were incubated overnight at 37 °C and 180 rpm. Chemicals were obtained from commercial sources (ciprofloxacin hydrochloride from Biomedicals no. 02199020, tobramycin sulfate salt from Serva no. 36562.01, meropenem trihydate from Sigma Aldrich no. M2574, L-arginine from AppliChem no. A3709). The compounds were diluted with LB media and distributed to the test plate just before the incubation with bacteria. The concentration ranges for the antibiotics were selected according to MIC values reported on the EUCAST website, while literature data were adapted for arginine concentrations72 (link).
L arginine
Manufactured by AppliChem
Sourced in Germany
L-arginine is an amino acid that plays a crucial role in various biological processes. It serves as a precursor for the production of nitric oxide, which is important for cardiovascular function and immune system support. L-arginine is commonly used in laboratory settings to study its effects on cellular and physiological mechanisms.
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Lab products found in correlation
Gsk591, by Merck Group (1 mentions)
Sodium azide nan3, by Avantor (1 mentions)
Deuterium oxide 2h2o, by Cambridge Isotopes (1 mentions)
3 trimethylsilyl propionic acid 2 2 3 3 d4 sodium salt tsp, by Thermo Fisher Scientific (1 mentions)
Sodium phosphate, by Avantor (1 mentions)
Hydrochloric acid (hcl), by Avantor (1 mentions)
Sodium hydroxide (naoh), by Avantor (1 mentions)
Chloroform, by Avantor (1 mentions)
Adenosine, by Merck Group (1 mentions)
2 protocols using l arginine
1
Pseudomonas aeruginosa PA14 Biofilm Mutants
2
Quantitative Metabolite Profiling
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L-arginine was obtained from AppliChem (Germany), ω-N G , N G -asymmetric dimethylarginine (ADMA), ω-N G -N' G -symmetric dimethylarginine (SDMA), and ω-N G -monomethylarginine (MMA) were obtained from Santa Cruz Biotechnology, Inc. (Germany). Sodium phosphate, dibasic (Na2HPO4), sodium hydroxide, chloroform, hydrochloric acid (32% m/v), and sodium azide (NaN3) were obtained from VWR International, 3-(trimethylsilyl) propionic acid-2,2,3,3-d4 sodium salt (TSP) from Alfa Aesar (Karlsruhe, Germany), deuterium oxide ( 2 H2O) from Cambridge Isotope laboratories, Inc. (Tewksbury, MA).
Adenosine, periodate oxidized (AdOx) and MS023 hydrochloride were obtained from Sigma Aldrich Austria. GSK591 (Synonyms: EPZ015866; GSK3203591 and GSK3368715 dihydrochloride (Synonyms: EPZ019997 dihydrochloride) were obtained from MedChemExpress Austria.
Adenosine, periodate oxidized (AdOx) and MS023 hydrochloride were obtained from Sigma Aldrich Austria. GSK591 (Synonyms: EPZ015866; GSK3203591 and GSK3368715 dihydrochloride (Synonyms: EPZ019997 dihydrochloride) were obtained from MedChemExpress Austria.
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