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Lsrfortessa x 20 flow

Manufactured by BD
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The LSRFortessa X-20 is a flow cytometer designed for high-performance cell analysis. It features a 20-parameter detection capability and is capable of analyzing a wide range of sample types, including cells, particles, and microorganisms.

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BD LSRFortessa X-20 flow cytometer BD LSRFortessa™ X-20 flow cytometer system BD LSRFortessa X-20 Cell Analyzer flow cytometer BD FACS LSRFortessa™ X-20 flow cytometer LSRFortessa X-20 Analyzer Flow Cytometer BD LSRFortessa X-20 SORP flow cytometer BD LSRFortessa™ X-20 flow cytometry LSRFortessa X-20 LSRFortessa

2 protocols using lsrfortessa x 20 flow

1

Cell Fixation and Flow Cytometry

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The samples were harvested and washed with FACS buffer containing 5% FBS for 3 times and fixed with 2% paraformaldehyde diluted with FACS buffer containing 5% FBS. The results were analyzed by Flow cytometry using BD LSRFortessa X-20 flow cytometer.
Xiang M., Li Y., Liu J., Shi J., Ge Y., Peng C., Bin Y., Wang Z, & Wang L. (2022). G-Quadruplex Linked DNA Guides Selective Transfection into Nucleolin-Overexpressing Cancer Cells. Pharmaceutics, 14(10), 2247.
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2

Trypanosome Cell Cycle Analysis

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Trypanosomes (1x105/ml) were cultured for 24 hours with deferoxamine at 2X IC50, gallic acid at ½ IC50, diminazene aceturate at ½ IC50 and harvested by centrifugation at 2700 rpm, then washed twice with PBS and fixed in 70% cold ethanol at -20°C overnight. The trypanosomes were then washed twice in PBS and 200 μl of guava cell cycle reagent (Sigma-Aldrich) was added and incubated in the dark for 30 minutes. Cell cycle analysis was done using the LSRFortessa X-20 flow (BD Biosciences) and FlowJo v10 software. At least 5000 trypanosomes were counted for each measurement.
Amisigo C.M., Antwi C.A., Adjimani J.P, & Gwira T.M. (2019). In vitro anti-trypanosomal effects of selected phenolic acids on Trypanosoma brucei. PLoS ONE, 14(5), e0216078.
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