Paraffin-embedded rat adrenal (catalog no. RP-501; Zyagen, San Diego, CA, USA) and brown fat (interscapular; catalog no. RP-131; Zyagen) were used as positive controls for NE and UCP1, respectively. Slides were submerged twice in HistoChoice Clearing Agent (catalog no. H103; Amresco, Solon, OH, USA), four times in 100% isopropanol, and twice in dH2O, for 3 min each. Slides were submerged and microwaved in 1% antigen unmasking solution (catalog no. H-330; Vector Laboratories, Burlingame, CA, USA) for antigen retrieval and rinsed in dH2O. NE primary antibody [1:250 (Ab8887, Abcam, Cambridge, UK)] or UCP1 primary antibody [1:125 (MAB6158; R&D Systems, Minneapolis, MN, USA)] was added for 1 hour at 37 °C in a humidified chamber. Slides were developed using 3, 3-diaminobenzidine (catalog no. SK-4100; Vector) for 2 minutes. Hematoxylin QS (catalog no. H-3404; Vector) was added for 30 s as a counterstain. Coverslips were mounted using Vectamount (catalog no. H-5000; Vector). Imaging was performed on a Nikon TE2000 inverted microscope with MMI Cell Tools (Molecular Machines & Industries, Zurich, Switzerland).
Ab8887
Manufactured by Abcam
Sourced in United Kingdom
Ab8887 is a primary antibody that recognizes the human PD-L1 protein. This antibody can be used for applications such as immunohistochemistry and western blotting.
Automatically generated - may contain errors
Lab products found in correlation
Mab6158, by R&D Systems (1 mentions)
Hematoxylin qs, by Vector Laboratories (1 mentions)
Antigen unmasking solution, by Vector Laboratories (1 mentions)
Histochoice clearing agent, by Avantor (1 mentions)
Vectamount, by Vector Laboratories (1 mentions)
Dp26 digital camera, by Olympus (1 mentions)
A10042, by Thermo Fisher Scientific (1 mentions)
Bx51 microscope, by Olympus (1 mentions)
2 protocols using ab8887
1
Immunohistochemistry of Adrenal and Brown Fat
2
Immunofluorescent Labeling of Noradrenalin
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Labeling with immunofluorescent antibodies was done as described previously with minor modifications. Sections were boiled in citrate buffer using a microwave oven and incubated in primary antibody (polyclonal rabbit‐anti‐noradrenalin antibody, 1:100, ab8887, Abcam). Negative controls were incubated in TBST with 5% skimmed milk without added primary antibody. Sections incubated with 4′,6‐diamidino‐2‐phenylindole, dihydrochloride (1:1000, not shown), and fluorescent secondary antibody (polyclonal donkey anti‐rabbit Alexa 568 antibody, 1:500, A10042, Thermo‐Fisher Scientific, Waltham, MA) dissolved in TBST. Sections were washed in TBST and mounted with DakoCytomation fluorescent mounting medium. Sections were analyzed with an pE‐300white LED Illumination Systems (Cool LED) equipped Olympus BX51 microscope and images acquired with an Olympus DP26 digital camera.
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