The viability of PTX-resistant SKOV3 and HeyA8 was analyzed using MTT detection kit (Beyotime, Jiangsu, China). In brief, cells were seeded in 96-well plates (5 × 103 per well) and cultured for 16 h. The cells were treated with test compounds for the defined time, and MTT solution (Beyotime) was added into each culture well. Four hours later, dimethyl sulfoxide (Millipore) was adopted to dissolve formazan. Finally, cell viability was analyzed using microplate reader (Thermo Labsystems, Waltham, MA, USA) with absorbance at 570 nm.
Mtt detection kit
Manufactured by Beyotime
Sourced in China
The MTT detection kit is a laboratory tool used to measure the metabolic activity of cells. It provides a colorimetric assay that can be used to quantify cell viability and proliferation. The kit contains the necessary reagents for performing the MTT assay.
Automatically generated - may contain errors
Lab products found in correlation
Microplate reader, by Thermo Fisher Scientific (4 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (2 mentions)
Mtt solution, by Beyotime (2 mentions)
Nanodrop 2000 spectrophotometer, by Thermo Fisher Scientific (2 mentions)
Abi 7300 real time pcr instrument, by Thermo Fisher Scientific (1 mentions)
Horseradish peroxidase labelled secondary antibody, by Santa Cruz Biotechnology (1 mentions)
Tunel apoptosis detection kit, by Beyotime (1 mentions)
Trizol, by Thermo Fisher Scientific (1 mentions)
Dual luciferase reporter assay system, by Promega (1 mentions)
Facscalibur flow cytometer, by BD (1 mentions)
M mlv reverse transcriptase, by Promega (1 mentions)
Inverted fluorescence microscope, by Olympus (1 mentions)
Annexin 5 apoptosis detection kit, by BD (1 mentions)
Ecl detection kit, by Beyotime (1 mentions)
Anti βactin antibody, by Keygen Biotech (1 mentions)
Ripa lysis buffer, by Beyotime (1 mentions)
Bca kit, by Beyotime (1 mentions)
5 protocols using mtt detection kit
1
MTT-based Cell Viability Assay
2
MTT Assay for Cell Viability
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MTT detection kit (C0009, Beyotime, China) was used in this section. MTT solution (10 μL) was added to each well and the cells were incubated for 4 h. Formazan (100 μL) was added and continued to incubate until dissolved. The OD value at 570 nm was measured by a microplate reader (800TS, BIOTEK, USA).
3
Cell Viability Assay for IC50 Determination
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MTT detection kit (Beyotime, Shanghai, China) was employed to detect cell viability and determine the half maximal inhibitory concentration (IC50) of SEV. In short, NCM cells, SW620 cells or SE480 cells were grown in 96-well plate for 18 h and treated with the desired treatments. After 24 h, cells were incubated with MTT solution (Beyotime) for 4 h in an incubator. Then, dimethyl sulfoxide (Millipore) was incubated with cells at 37°C for 4 h. Cell viability was determined by analyzing the absorbance at 570 nm using a microplate reader (Thermo Labsystems, Waltham, MA, USA). The concentration of SEV causing 50% inhibition of growth (IC50) was determined by assessing the cell viability.
4
Evaluating miR-24 Regulatory Mechanisms
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TRIzol, Lipofectamin™ 2000 transfection Kit (#11668027), and Power SYBR Green PCR Kit (#4309155) were purchased from Invitrogen, USA. Dual-Luciferase Reporter Assay System and M-MLV reverse transcriptase were obtained from Promega. The mirVana™ qRT-PCR miRNA Detection Kit (#AM1558) was acquired from Life Technologies, USA. The miR-24 mimics and inhibitors were synthesised by the GenePharma Company (Shanghai, China). MTT Detection Kit and TUNEL Apoptosis Detection Kit were procured from Beyotime Institute of Biotechnology (Beijing, China). Lactate dehydrogenase (LDH) and creatine phosphokinase (CPK) detection kits were procured from the Nanjing Jiancheng Bioengineering Institute, China. Annexin V Apoptosis Detection Kit was purchased from BD Biosciences, USA. Bim and GAPDH antibodies were purchased from Cell Signaling. The horseradish peroxidase-labelled secondary antibody was procured from Santa Cruz Biotechnology. The enzyme label instrument, Tecan Infinite M1000, was procured from Switzerland. The ABI 7300 real-time PCR instrument was purchased from Applied Biosystems, USA. The NanoDrop 2000 spectrophotometer was purchased from Thermo, USA. The FacsCalibur flow cytometer was purchased from BD Biosciences, USA. The inverted fluorescence microscope was purchased from Olympus, Japan. The upfront fluorescence microscope was purchased from Leica, Germany.
5
Immunofluorescence Labeling Optimization Protocol
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