Mitotracker staining solution

According to the departmental protocols, the effect of SeNPs supplementation on mitochondrial dysfunctions in renal cells induced by in vitro NaAsO₂ exposure was analyzed by assessing mitochondrial activity and Mitochondrial Membrane Potentials (ΔΨm) of renal cells by MitoTracker staining and JC-1 staining.8 (link)
For MitoTracker staining, HK2 cells after drug treatments were thoroughly washed with Dulbecco’s phosphate buffered saline solution (DPBS, 14190250, Thermo Fisher Scientific, Beijing, China) and incubated with 4% paraformaldehyde solution (PFA, P1110, Solarbio, Beijing, China) at room temperature for 15 min. Subsequently, HK2 cells from each group were incubated with 200 nM MitoTracker staining solution (C1049, Beyotime, Shanghai, China) at 37 °C for 30 min. After incubation of 10 μg/mL DAPI staining solution (C0065, Solarbio, Beijing, China), the MitoTracker staining density of each group was analyzed by Image J software.
For JC-1 staining, HK2 cells after PFA fixation were incubated with 10 μM JC-1 staining solution (C2006, Beyotime, Shanghai, Zhejiang, China) at 37 °C for 20 min, followed by further incubation of DAPI staining solution and subsequent analysis of staining intensity by Image J software.

The effect of Cu-CDs treatments on mitochondrial dysfunctions of breast cancer cells was confirmed by assessing the mitochondrial activity and mitochondrial membrane potentials (ΔΨm) of MDA-MB-231 cells by MitoTracker staining and JC-1 staining.52 (link)
For MitoTracker staining, MDA-MB-231 cells after PFA fixation were washed with DPBS solution and incubated with 200 nM MitoTracker staining solution (C1049, Beyotime, Shanghai, China) at 37 °C for 30 min. After DAPI staining, the MitoTracker staining was recorded with the MitoTracker staining intensity analyzed by Image J software.
For JC-1 staining, MDA-MB-231 cells were washed with DPBS solution and incubated with 10 μM JC-1 staining solution (C2006, Beyotime, Shanghai, China) at 37 °C for 20 min. Subsequently, the staining signals of J-aggregate (green staining intensity) and J-monomer (red staining intensity) were recorded with ΔΨm potentials (relative J-aggregate/J-monomer staining intensity ratio) analyzed by Image J software.