H 2db pe

Manufactured by BioLegend

The H-2Db PE is a fluorescently-labeled antibody that specifically binds to the H-2Db molecule, which is a major histocompatibility complex (MHC) class I molecule expressed on the surface of cells. This product is intended for use in flow cytometry applications to identify and analyze H-2Db-expressing cells.

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Lab products found in correlation

Cd45.1 fitc, by Thermo Fisher Scientific (1 mentions) Live dead near ir, by Thermo Fisher Scientific (1 mentions) Tcrβ apc, by Thermo Fisher Scientific (1 mentions) B220 pe texasred, by Thermo Fisher Scientific (1 mentions) Cyan adp, by Agilent Technologies (1 mentions) Celltrace violet, by Thermo Fisher Scientific (1 mentions) Canto 2, by BD (1 mentions) Fortessa x20, by BD (1 mentions) Cd8 apc efluor 780, by Thermo Fisher Scientific (1 mentions) Cd44 buv737, by BD (1 mentions) Cd62l bv786, by BD (1 mentions) H2kb pecy7, by Thermo Fisher Scientific (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Merck Group (1 mentions)

Close spelling variants of this product

PE anti-mouse H-2Kb/H-2Db H-2Db

2 protocols using h 2db pe

Multicolor Flow Cytometry Analysis

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The following monoclonal antibodies and cell dyes were used: CD45.2 PE-Cy7, CD45.1 FITC, TCRβ APC, B220 PE-TexasRed (all eBioscience), H-2Db PE (BioLegend), LiveDead nearIR and CellTrace Violet (both Invitrogen), and H-2Db-ASNENMDAM dextramer-PE (Immudex). Samples were acquired on CyAn ADP (Dako Cytomation), Canto-II (BD) or Fortessa X20 (BD) flow cytometers, and analysis was performed with FlowJo software (Treestar).

Hogan T., Kadolsky U., Tung S., Seddon B, & Yates A. (2014). Spatial Heterogeneity and Peptide Availability Determine CTL Killing Efficiency In Vivo. PLoS Computational Biology, 10(9), e1003805.

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Comprehensive Immunophenotyping Protocol

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Antibodies for flow cytometry and FACS: H2Kb PECy7 (eBioscience 25-5958-82), H2Db PE (BioLegend 111508), I-A/I-E (MHCII) BV711 (BD Biosciences 563414), PDL1 BV41 (BD Biosciences 564716), CD8 APC-eFluor 780 (eBioscience 47-0081-82), DAPI (Sigma, D9564-10 MG), PD1 PerCP-eFluor710 (Invitrogen 46-9985-82), CD44 BUV737 (BD Biosciences 564392), CD62 L BV786 (BD Biosciences 564109), hTCRab PerCP-Cy5.5 (Biolegend 306724), hCD3 PECy7 (BD Biosciences 563423). p15E (KSPWFTTL), OVA (SIINFEKL) peptides and corresponding pentamers were from ProImmune.

Ye X., Waite J.C., Dhanik A., Gupta N., Zhong M., Adler C., Malahias E., Ni M., Wei Y., Gurer C., Zhang W., Macdonald L.E., Murphy A.J., Sleeman M.A, & Skokos D. (2020). Endogenous retroviral proteins provide an immunodominant but not requisite antigen in a murine immunotherapy tumor model. Oncoimmunology, 9(1), 1758602.

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