Wll tcs sp8 confocal laser scanning microscope

Manufactured by Leica

Sourced in United States

The Leica WLL TCS SP8 Confocal Laser Scanning Microscope is a high-performance imaging system designed for advanced microscopy applications. It utilizes a multi-laser configuration and a specialized optical system to capture high-resolution, detailed images of microscopic samples.

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Lab products found in correlation

Dmi4000b, by Leica (1 mentions) Dm4000b microscope, by Leica (1 mentions)

Close spelling variants of this product

TCS SP8 laser confocal microscope TCS SP8 WLL SP8 laser confocal microscope WLL Leica SP8 microscope Confocal laser scanning microscope TCS SP8 laser Confocal TCS SP8 TCS SP8 microscope SP8 WLL SP8 confocal microscope

5 protocols using wll tcs sp8 confocal laser scanning microscope

Fluorescence and Confocal Microscopy Imaging

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Fluorescent microscopy was conducted using a Leica DMI4000 B automated inverse fluorescence microscope. Confocal microscopy was conducted using a Leica WLL TCS SP8 Confocal Laser Scanning Microscope (Stanford Cell Sciences Imaging Facility, purchased with NIH grant 1510OD01058001A1). For confocal imaging, the working distance and numerical aperature (NA) for the objectives used are as follows: 20X (680 m with oil immersion, NA = 0.75) and 40X (240 μm with oil immersion, NA = 1.30). Confocal imaging was conducted with a 2048 × 2048 pixel format and 200 Hz speed (as noted above). Z-stack step size was 0.3 μm. Precise excitation and hybrid detection of the fluorophores found in each mouse model used were captured. Z-stack images were rendered into 3-dimensions for analysis.

Foster D.S., Nguyen A.T., Chinta M., Salhotra A., Jones R.E., Mascharak S., Titan A.L., Ransom R.C., da Silva O.L., Foley E., Briger E, & Longaker M.T. (2019). A Clearing Technique to Enhance Endogenous Fluorophores in Skin and Soft Tissue. Scientific Reports, 9, 15791.

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Multi-Fluorescent Confocal Imaging

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Using a Leica WLL TCS SP8 Confocal Laser Scanning Microscope, we imaged the 10 micron cryosections and whole tissue mounts with ×20, ×40, and ×63 oil objectives. Precise excitation and hybrid detection of mCerulean, eGFP, mOrange, and mCherry was captured and documented. Z-stacked confocal images were rendered into 3-dimensions for further analysis.

Ransom R.C., Foster D.S., Salhotra A., Jones R.E., Marshall C.D., Leavitt T., Murphy M.P., Moore A.L., Blackshear C.P., Brett E.A., Wan D.C, & Longaker M.T. (2018). Genetic dissection of clonal lineage relationships with hydroxytamoxifen liposomes. Nature Communications, 9, 2971.

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Confocal Microscopy for Tissue Analysis

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Laser scanning confocal microscopy was performed using a Leica WLL TCS SP8 Confocal Laser Scanning Microscope (Leica Microsystems) located in the Cell Sciences Imaging Facility (Stanford University, Stanford, CA, USA). The ×10, ×20, and ×40 objectives were used (×10 HC PL APO, air, N.A. 0.40; ×20 and ×40 HC PL APO IMM CORR CS2, H2O/Glycerol/oil, N.A. 0.75). Raw image stacks were imported into Fiji (Image-J, NIH) or Imaris (Bitplane) software for analysis. Fiji was used to make two-dimensional micrographs of the confocal data and to quantify fluorophore expression intensity. For analysis of clonality from rainbow mouse tissue, surfaces were created for each color of the rainbow construct expressed using the volume surface and thresholding tools in Imaris.
Fluorescent and bright-field images were taken with a Leica DM4000B microscope (Leica Microsystems) and RETIGA 2000R camera (QImaging Scientific Cameras).

Maan Z.N., Rinkevich Y., Barrera J., Chen K., Henn D., Foster D., Bonham C.A., Padmanabhan J., Sivaraj D., Duscher D., Hu M., Yan K., Januszyk M., Longaker M.T., Weissman I.L, & Gurtner G.C. (2021). Epidermal-Derived Hedgehog Signaling Drives Mesenchymal Proliferation during Digit Tip Regeneration. Journal of Clinical Medicine, 10(18), 4261.

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Confocal Microscopy Protocol for Rainbow Mouse

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Laser scanning confocal microscopy was performed using a Leica WLL TCS SP8 Confocal Laser Scanning Microscope (Leica Microsystems) located in the Cell Sciences Imaging Facility (Stanford University, Stanford, CA). The ×10, ×20, and ×40 objectives were used (×10 HC PL APO, air, N.A. 0.40; ×20 and ×40 HC PL APO IMM CORR CS2, H2O/Glycerol/oil, N.A. 0.75). Raw image stacks were imported into Fiji (Image-J, NIH) or Imaris (Bitplane) software for analysis. Fiji was used to make two-dimensional micrographs of the confocal data and to quantify fluorophore expression intensity. For analysis of clonality from Rainbow mouse tissue, surfaces were created for each color of the Rainbow construct expressed using the volume surface and thresholding tools in Imaris.

Foster D.S., Marshall C.D., Gulati G.S., Chinta M.S., Nguyen A., Salhotra A., Jones R.E., Burcham A., Lerbs T., Cui L., King M.E., Titan A.L., Ransom R.C., Manjunath A., Hu M.S., Blackshear C.P., Mascharak S., Moore A.L., Norton J.A., Kin C.J., Shelton A.A., Januszyk M., Gurtner G.C., Wernig G, & Longaker M.T. (2020). Elucidating the fundamental fibrotic processes driving abdominal adhesion formation. Nature Communications, 11, 4061.

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Laser Confocal Microscopy for Multicolor Fluorescence Analysis

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Tissues were fixed and prepared in the dark to minimize bleaching of endogenous fluorophore expression. Laser scanning confocal microscopy was performed using a Leica WLL TCS SP8 Confocal Laser Scanning Microscope (Leica Microsystems) located in the Cell Sciences Imaging Facility (Stanford University, Stanford, CA). The ×10, ×20, and ×40 objectives were used (×10 HC PL APO, air, N.A. 0.40; ×20 and ×40 HC PL APO IMM CORR CS2, H2O/Glycerol/oil, N.A. 0.75). Precise excitation and hybrid detection of the Rainbow fluorophores (mCerulean, eGFP, mOrange, and mCherry) was captured when applicable. Raw image stacks were imported into Fiji (Image-J, NIH) or Imaris (Bitplane) software for analysis. Fiji was used to make two-dimensional micrographs of the confocal data and to quantify fluorophore expression intensity. For analysis of clonality from Rainbow mouse tissue, surfaces were created for each color of the Rainbow construct expressed using the volume surface and thresholding tools in using Imaris software.

Foster D.S., Januszyk M., Delitto D., Yost K.E., Griffin M., Guo J., Guardino N., Delitto A.E., Chinta M., Burcham A.R., Nguyen A.T., Bauer-Rowe K.E., Titan A.L., Salhotra A., Jones R.E., da Silva O., Lindsay H.G., Berry C.E., Chen K., Henn D., Mascharak S., Talbott H.E., Kim A., Nosrati F., Sivaraj D., Ransom R.C., Matthews M., Khan A., Wagh D., Coller J., Gurtner G.C., Wan D.C., Wapnir I.L., Chang H.Y., Norton J.A, & Longaker M.T. (2022). Multiomic analysis reveals conservation of cancer associated fibroblast phenotypes across species and tissue of origin. Cancer cell, 40(11), 1392-1406.e7.

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