Complete f12 dulbecco s modified eagle medium

Manufactured by Thermo Fisher Scientific

Complete F12-Dulbecco's Modified Eagle Medium is a cell culture medium formulated for the growth and maintenance of a variety of cell types. It provides a balanced set of nutrients and growth factors required for cell proliferation and survival.

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Lab products found in correlation

Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions) Penicillin streptomycin antibiotic, by Thermo Fisher Scientific (2 mentions) Protein assay kit 2, by Bio-Rad (1 mentions) Sk n sh human neuroblastoma cells, by American Type Culture Collection (1 mentions)

Spelling variants of this product

F12 medium (343 citations) Dulbecco’s modified (295 citations) Complete medium (38 citations) Complete Dulbecco's modified Eagle's medium (32 citations) Complete Dulbecco’s Modified Eagle Medium (7 citations)

2 protocols using complete f12 dulbecco s modified eagle medium

Neuroblastoma Cell Culture and CaMKII Assay

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SK-N-SH human neuroblastoma cells (43 ) obtained from American Type Culture Collection (ATCC HTB-11, Manassas, VA) were grown in complete F12-Dulbecco's Modified Eagle Medium (ThermoFisher Scientific) as previously described (13 (link)). Complete media contained 10% fetal bovine serum (ThermoFisher Scientific) and 1% penicillin-streptomycin antibiotic (ThermoFisher Scientific). Cellular extracts used in the CaMKII assay were centrifuged at 15,000 rpm for 20 min at 4°C. Protein concentrations of the extracts were determined by Bradford assay using the Protein Assay Kit II (Bio-Rad, Hercules, CA) and used to determine specific activity of CaMKII.

Chain J.L., Alvarez K., Mascaro-Blanco A., Reim S., Bentley R., Hommer R., Grant P., Leckman J.F., Kawikova I., Williams K., Stoner J.A., Swedo S.E, & Cunningham M.W. (2020). Autoantibody Biomarkers for Basal Ganglia Encephalitis in Sydenham Chorea and Pediatric Autoimmune Neuropsychiatric Disorder Associated With Streptococcal Infections. Frontiers in Psychiatry, 11, 564.

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Culturing Human Neuroblastoma Cells

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SK-N-SH human neuroblastoma cells (Biedler et al., 1973 ) obtained from American Type Culture Collection (ATCC HTB-11, Manassas, VA) were grown in complete F12-Dulbecco’s Modified Eagle Medium (ThermoFisher Scientific) as previously described (Kirvan et al., 2003 (link)). Complete media contained 10% fetal bovine serum (ThermoFisher Scientific) and 1% penicillin-streptomycin antibiotic (ThermoFisher Scientific).

Fallon B.A., Strobino B., Reim S., Stoner J, & Cunningham M.W. (2020). Anti-lysoganglioside and other anti-neuronal autoantibodies in post-treatment Lyme Disease and Erythema Migrans after repeat infection. Brain, Behavior, & Immunity - Health, 2, 100015.

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