Trypan blue dye

Manufactured by Merck Group

Sourced in United States, Germany, United Kingdom, Italy, Belgium, Australia

Trypan blue dye is a vital staining dye used in cell counting and viability assays. It is a blue-colored dye that penetrates the cell membrane of dead or dying cells, allowing them to be easily identified and differentiated from live, healthy cells under a microscope.

Automatically generated - may contain errors

Lab products found in correlation

Dimethyl sulfoxide (dmso), by Merck Group (25 mentions) L glutamine, by Lonza (17 mentions) Gentamycin, by Lonza (16 mentions) Fetal bovine serum (fbs), by Lonza (16 mentions) Rpmi 1640, by Lonza (13 mentions) Crystal violet, by Merck Group (13 mentions) Hepes buffer solution, by Lonza (12 mentions) Dulbecco modified eagle medium (dmem), by Lonza (12 mentions) Trypsin edta, by Lonza (12 mentions) Penicillin, by Merck Group (8 mentions) Streptomycin, by Merck Group (8 mentions) 3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt, by Merck Group (7 mentions) Trypsin edta, by Thermo Fisher Scientific (7 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (7 mentions) Ascorbic acid, by Merck Group (6 mentions) L glutamine, by Merck Group (5 mentions) Trypsin, by Merck Group (5 mentions) Reverse phase microscope, by Nikon (4 mentions) Rpmi 1640 medium, by Merck Group (4 mentions) Dulbecco s modified eagle s medium dmem, by Merck Group (4 mentions)

Close spelling variants of this product

Trypan blue vital cell dye (2 citations) Trypan blue vital dye (9 citations) Trypan blue dye solution (5 citations) Trypan blue dye exclusion (56 citations) Trypan blue dye exclusion method (5 citations) Trypan blue dye exclusion test (11 citations) Trypan blue dye exclusion assay (12 citations) Trypan blue (1976 citations) TM-blue (2 citations)

211 protocols using trypan blue dye

Trypan Blue Cell Viability Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

To compare the number of dead cells in four PRODH-expressing clones and two control clones, we performed an initial analysis by staining cells with Trypan Blue dye (Sigma Aldrich, Milan, Italy). Cells were seeded in a 6-well plate to reach 80% confluence after four days from seeding. On day four, cells were detached and an aliquot of the cellular suspension was diluted 1:2 with Trypan Blue dye; the cells were counted with Burker chamber using a light microscope (TIEsseLab, Milan, Italy). The test was repeated twice, and at least 700 cells were counted for each clone.

Grossi S., Berno E., Chiofalo P., Chiaravalli A.M., Cinquetti R., Bruno A., Palano M.T., Gallazzi M., La Rosa S., Sessa F., Acquati F, & Campomenosi P. (2024). Proline Dehydrogenase (PRODH) Is Expressed in Lung Adenocarcinoma and Modulates Cell Survival and 3D Growth by Inducing Cellular Senescence. International Journal of Molecular Sciences, 25(2), 714.

+ Open protocol

+ Expand

Porous Hydrogel Scaffold Fabrication

Check if the same lab product or an alternative is used in the 5 most similar protocols

Gelatin type A from porcine
skin (bloom 300), GA (25% solution in water), glycine, 1-ethyl-3-(3-dimethyl-aminopropyl)carbodiimidehydrochloride
(EDC), diethylpyrocarbonate, glycyrrhetinic acid, and collagenase
from Clostridium histolyticum in addition
to the chemicals that were used in the cytotoxicity studies: dimethyl
sulfoxide (DMSO), MTT, and trypan blue dye were purchased from Sigma,
St. Louis, Mo., USA. Acetone was purchased from Labscan, Dublin, Ireland.
Allicin was purchased from Jiangsu chiataiqingjiang Pharmaceutical
Co., Ltd., China. Spectra/Por dialysis membrane, 12 000–14 000
molecular weight cutoff, was purchased from Spectrum Laboratories
Inc., Rancho Dominguez, Canada.

Ossama M., Hathout R.M., Attia D.A, & Mortada N.D. (2019). Enhanced Allicin Cytotoxicity on HEPG-2 Cells Using Glycyrrhetinic Acid Surface-Decorated Gelatin Nanoparticles. ACS Omega, 4(6), 11293-11300.

+ Open protocol

+ Expand

Quantitative HPLC Analysis of Bioactive Compounds

Check if the same lab product or an alternative is used in the 5 most similar protocols

Ursolic acid, salvianolic acid A, salvianolic acid B, Danshensu, and Protocatechuic aldehyde (purity > 99%) were supplied by the National Institute for the Control of Pharmaceutical and Biological Products (Beijing, China). Acetonitrile (ACN) used in high performance liquid chromatography (HPLC) was purchased from Fisher Scientific (Pittsburgh, PA. USA). Phosphoric acid and ethanol (analytical grade) were obtained from Beijing Beihua Fine Chemicals (Beijing, China). Deionized water was purified by the Milli-Q System (Millipore, Bedford, MA, USA). Penicillin, streptomycin, and fetal bovine serum (FBS) were purchased from Hyclone (Logan, UT, USA). Lipopolysaccharide (LPS) and Dulbecco’s modified Eagle’s medium (DMEM) were purchased from Gibco BRL (Grand Island, NY, USA). 3-(4,5- dimethylthiazol − 2-yl)-2,5-diphenyltetrazolium bromide (MTT), 2,3,5-triphenyl tetrazolium chloride (TTC), and Trypan blue dye were obtained from Sigma (St. Louis, MO, USA). All other chemicals and solvents used were purchased from Sinopharm Chemical (Shanghai, China) and all of them were of analytical grade or better.

Wu S., Wang Q., Wang J., Duan B., Tang Q., Sun Z., Han J., Shan C., Wang Z, & Hao Z. (2020). Protocatechuic aldehyde from Salvia miltiorrhiza exhibits an anti-inflammatory effect through inhibiting MAPK signalling pathway. BMC Complementary Medicine and Therapies, 20, 347.

+ Open protocol

+ Expand

Culturing Primary Neurosphere Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

We modified a previously published method [30 , 31 (link)]. To culture primary neurosphere cells, C57BL/6 mice pups, postnatal day-3, were sacrificed by decapitation. Dissections were performed in PBS with 0.6% glucose, pH 7.4, at 4°C using sterile scissor and forceps. For the SVZ dissections, each brain was sliced into 2 mm coronal sections. Tissue was washed in PBS/glucose, digested in papain–dispase–DNase solution (Lorne Laboratories, Twyford, UK); 0.1% dispase (Roche Diagnostics, Hertfordshire, UK) for 4 min, filtered through 40 μm nylon mesh cell filters (Falcon, Suffolk, UK), and then washed three times. Live cells were calculated using the trypan blue dye (Sigma) exclusion method and seeded at a density of 1 x 10⁵ cells/ml in 25 cm² flasks in DMEM/F12 with B27 (Invitrogen, Eugene, Oregon, USA), 1% penicillin /streptomycin/fungizone (Invitrogen, Eugene, Oregon, USA), 20 ng/ml bFGF and 20 ng/ml EGF (R&D Systems, MN, USA).

Ban J.J., Yang S., Im W, & Kim M. (2016). Neurogenic Effects of Cell-Free Extracts of Adipose Stem Cells. PLoS ONE, 11(2), e0148691.

+ Open protocol

+ Expand

Quantification of hGFs in Cell Culture

Check if the same lab product or an alternative is used in the 5 most similar protocols

To assess the total number of cells, 0.2 × 10⁶ cells/mL of hGFs were placed in six well plates (Euroclone), in duplicate, for the wound-healing assay. The medium was removed and replaced by 0.25% trypsin/0.1% EDTA 1× solution (Merck KGaA) for 2 min to promote cell detachment from the acrylic substrate. In order to stop trypsin activity, the same amount of complete medium was added, and cells were collected and centrifuged at 1200× g for 5 min in order to obtain a pellet. The pellet was resuspended in 2 mL of DMEM and cells were counted using a Bürker chamber and a DM IL inverted light microscope (Leica Camera AG, Wetzlar, Germany). The number of cells obtained in the counting corresponds to the number of cells per milliliter of suspension. Cell viability was evaluated by Trypan blue dye exclusion. A total of 90 μL of 0.04% Trypan blue dye (Sigma-Aldrich Corp., St. Louis, MO, USA) was added to 10 μL of cell suspension and examined to determine the number of viable cells. The total number of living cells in the culture amounted to over 98% of viable cells, as determined by the exclusion of the blue Trypan dye.

Costantini E., Sinjari B., D’Angelo C., Murmura G., Reale M, & Caputi S. (2019). Human Gingival Fibroblasts Exposed to Extremely Low-Frequency Electromagnetic Fields: In Vitro Model of Wound-Healing Improvement. International Journal of Molecular Sciences, 20(9), 2108.

+ Open protocol

+ Expand

Cytotoxicity Evaluation of Cancer Cell Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols

MCF-7 cells (human breast cancer cell line) and HCT-116 cells (human colon carcinoma cell line) were obtained from VACSERA Tissue Culture Unit. Dimethyl sulfoxide (DMSO), crystal violet, and trypan blue dye were purchased from Sigma (St. Louis, Mo., USA). Fetal bovine serum, DMEM, RPMI-1640, HEPES buffer solution, L-glutamine, gentamycin, and 0.25% Trypsin-EDTA were purchased from Lonza. crystal violet stain (1%) composed of 0.5% (w/v) crystal violet and 50% methanol dissolved in ddH₂O and filtered through a Whatmann No.1 filter paper. The cytotoxicity test was conducted according the method described by Mosmann, 1983 (link), Riyadh et al., 2015 .

Alkhulaifi M.M., Alshehri J.H., Alwehaibi M.A., Awad M.A., Al-Enazi N.M., Aldosari N.S., Hatamleh A.A, & Abdel- Raouf N. (2020). Green synthesis of silver nanoparticles using Citrus limon peels and evaluation of their antibacterial and cytotoxic properties. Saudi Journal of Biological Sciences, 27(12), 3434-3441.

+ Open protocol

+ Expand

Cell Viability Assay Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

HEPES buffer, Dulbecco’s Modified Eagle’s Medium (DMEM), DMEM:Ham’s F12 medium, streptomycin/penicillin mixture, phosphate-buffered saline (PBS, pH 7.2), trypan blue dye, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), dimethyl sulfoxide (DMSO) derived from Sigma-Aldrich (St. Louis, MO, USA). Foetal bovine serum (FBS), GlutaMAX^TM, TrypLE^TM Express originated from Invitrogen Thermo Fisher Scientific (USA); 0.22 μm pore size syringe filters were from Merck Millipore (Germany).

Radzimierska-Kaźmierczak M., Śmigielski K., Sikora M., Nowak A., Plucińska A., Kunicka-Styczyńska A, & Czarnecka-Chrebelska K.H. (2021). Olive Oil with Ozone-Modified Properties and Its Application. Molecules, 26(11), 3074.

+ Open protocol

+ Expand

Cytotoxicity and Cellular Oxidative Stress in CHO-K1 Cells Exposed to PCB 153

Check if the same lab product or an alternative is used in the 5 most similar protocols

The Chinese hamster ovary cell line (CHO-K1) was purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA). Dulbecco’s MEM F-12 (DMEM F-12), heat inactivated foetal bovine serum (FBS), and trypsin/EDTA solution (0.25 % trypsin with EDTA 4Na) were supplied by GIBCO (Paisley, UK). PCB 153 (2,2’,4,4’,5,5’-hexachlorobiphenyl, CAS 35065-27-1), Trypan Blue dye (CAS 72-57-1), MTT (3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2 h-tetrazolium bromide, CAS 298-93-1), and propidium iodide (PI, CAS 25535-16-4) were obtained from Sigma-Aldrich (St. Louis, MO, USA). Coomassie brilliant blue R-250 (CAS 6104-59-2) was purchased from LKB (Bromma, Sweden). Neutral Red dye (CAS 553-24-2) and Muse™ Annexin V & Dead Cell Kit (cat #MCH100105) were supplied by Merck (Billerica, MA, USA). The 2’,7’-dichlorofluorescin diacetate (DCFDA) Cellular ROS Detection Assay Kit (cat #ab113851) was obtained from Abcam (Cambridge, UK). DMSO (CAS 67-68-5) and ethanol (CAS 64-17-5) were purchased from Kemika (Zagreb, Croatia).

Miletić M., Murati T., Šimić B., Bilandžić N., Brozović A, & Kmetič I. (2021). Ortho-substituted PCB 153: effects in CHO-K1 cells. Archives of Industrial Hygiene and Toxicology, 72(4), 326-332.

+ Open protocol

+ Expand

Cell Culture Conditions for p53 Studies

Check if the same lab product or an alternative is used in the 5 most similar protocols

MEF WT p53^+/+, MEF p53^−/−, SaOs2-tetracycline inducible p53, E1A-RAS transformed MEFs, HT1080, EB3, 293T, CA46, SupT1, MiaPaca2, and 249P cells were initially cultured in Dulbecco’s modified Eagle’s medium (DMEM, Corning, New York, NY, USA) which contained 25 mM glucose and 4 mM L-glutamine. DMEM was supplemented with 10% dialyzed fetal bovine serum (dFBS, Gemini Bio-Products, Sacramento, CA, USA) and 100 units/mL penicillin and 100 μg/mL streptomycin (P/S, Genesee Scientific). All cells were cultured at 37° C with 5% CO₂. SaOs2 cells engineered to induce WT p53 upon tetracycline were generously provided by Karen Vousden (Francis Crick Institute, London, England) (Bensaad et al., 2006 (link)). Doxycycline (1 μg/mL) was added to DMEM 24 hours prior to glutamine deprivation studies to ensure p53 expression. BPTES (used at 10 μM) and camptothecin (CPT, used at 2 μM) was purchased from Sigma-Aldrich (St Louis, MO, USA). Doxorubicin was purchased from Selleckchem (Houston, TX, USA). Cell viability and proliferation was measured by exclusion of Trypan blue dye (Sigma) and determined by cell counting.

Lowman X.H., Hanse E.A., Yang Y., Ishak Gabra M.B., Tran T.Q., Li H, & Kong M. (2019). p53 Promotes Cancer Cell Adaptation to Glutamine Deprivation by Upregulating Slc7a3 to Increase Arginine Uptake. Cell reports, 26(11), 3051-3060.e4.

+ Open protocol

+ Expand

Cytotoxicity Assessment of Hib Compounds

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells were seeded in 6-well plates at 2.5 × 103 cells/well density and were treated after 24 h with increasing concentrations of HsEF, Hib-ester or Hib-carbaldehyde. Untreated cells represented the controls. After 24, 48 and 72 h, the cells were collected, stained with Trypan Blue dye (Sigma Aldrich, St. Louis, MO, USA) and counted in a hemocytometer. Both viable and dead cells were counted. The percentage of death cells was calculated on the sum of all counted cells.

Malacrida A., Cavalloro V., Martino E., Costa G., Ambrosio F.A., Alcaro S., Rigolio R., Cassetti A., Miloso M, & Collina S. (2021). Anti-Multiple Myeloma Potential of Secondary Metabolites from Hibiscus sabdariffa—Part 2. Molecules, 26(21), 6596.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!