Male C57BL/6N mice (approximately 7 weeks old; Charles River Laboratories Japan, Yokohama, Japan) were maintained under a 12 h light/ 12 h dark cycle, fed with a conventional CE-2 laboratory diet (CLEA Japan, Tokyo, Japan), and were provided with water ad libitum for 1 week for acclimatization. The mice were then intraperitoneally administered with PCN (100 mg/kg), followed 6 h later by CCl4 (0.5 mL/kg, 10% v/v, in corn oil). After 24 h, they were sacrificed, and the blood and liver samples were collected. Plasma alanine aminotransferase (ALT) activity was determined using the Transaminase CII-B-test Wako (FUJIFILM Wako Pure Chemical) following the manufacturer’s instructions. All animal experiments were approved by the committee for animal experiments at University of Shizuoka and conducted in accordance with the guidelines for animal experiments at University of Shizuoka.
Transaminase cii b test wako
Manufactured by Fujifilm
Sourced in Japan
The Transaminase CII-B-test Wako is a laboratory equipment product manufactured by Fujifilm. It is designed to measure the activity of the enzyme transaminase, which is important for various clinical diagnostic applications.
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Lab products found in correlation
3 protocols using transaminase cii b test wako
1
Liver Injury Induction in Mice
2
Hepatic Lipid Profiling Protocol
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Serum ALT and ALP activities were determined using the commercial kits, Transaminase CII-B-test Wako and LabAssay TM ALP (Wako Pure Chemicals, Osaka, Japan), respectively. The hepatic samples were prepared as described previously (Miyata et al., 2010) . Hepatic TGs, NEFAs, TC, and bile acids were determined using the Triglyceride E-test Wako, NEFA E-test Wako, and Cholesterol E-test Wako kits and the total bile acidtest Wako (Wako Pure Chemicals), respectively.
3
Hepatic Lipid Biochemical Analyses
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Serum ALT and ALP activities were determined using the commercial kits, Transaminase CII-B-test Wako and Alkali-phospha B-test Wako (Wako Pure Chemicals, Osaka, Japan), respectively. Hepatic samples were prepared as described previously (Miyata et al., 2010) . Hepatic TAG, FFA, and TC were determined using the Triglyceride E-test Wako, NEFA E-test Wako, and Cholesterol E-test Wako kits (Wako Pure Chemicals), respectively.
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