Ccl 33

Manufactured by American Type Culture Collection

Sourced in United States

The CCL-33 is a cell line derived from human embryonic kidney cells. It is a commonly used cell line for various research and experimental applications. The core function of the CCL-33 is to serve as a reliable and well-characterized cell model for in vitro studies.

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Lab products found in correlation

Crl 3216, by American Type Culture Collection (3 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (3 mentions) Crl 2935, by American Type Culture Collection (2 mentions) Penicillin g, by Avantor (2 mentions) Amphotericin b, by Avantor (2 mentions) Opti mem, by Thermo Fisher Scientific (2 mentions) Dulbecco modified eagle medium (dmem), by Merck Group (2 mentions) L glutamine, by Merck Group (2 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions) Dmem f12, by Thermo Fisher Scientific (2 mentions) Glutamax, by Thermo Fisher Scientific (2 mentions) Minimum essential medium (mem), by Thermo Fisher Scientific (1 mentions) Hek293, by American Type Culture Collection (1 mentions) Ibrs 2, by Thermo Fisher Scientific (1 mentions) Tib 202, by American Type Culture Collection (1 mentions) Thp 1, by Thermo Fisher Scientific (1 mentions) Rpmi 1640, by Thermo Fisher Scientific (1 mentions) Hb 8065, by American Type Culture Collection (1 mentions) William s e medium, by Thermo Fisher Scientific (1 mentions) Penicillin streptomycin, by American Type Culture Collection (1 mentions)

8 protocols using ccl 33

Cell Culture Conditions for Cell Lines

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Porcine kidney (PK-15) (ATCC#CCL-33), human embryonic kidney 293 (HEK293) (ATCC #CRL-1573), human monocytic cell line (THP-1) (ATCC #TIB-202), and Instituto Biologico-Rim Suino-2 (IBRS-2) (ATCC #CRL-1835) cells were purchased from the American Type Culture Collection (USA). PK-15 and IBRS-2 cells were cultured in minimum essential medium (MEM, Gibco, USA) supplemented with 10% heat-inactivated FBS, penicillin (100 U/mL), and streptomycin sulfate (100 μg/mL). Human embryonic kidney 293 (HEK293) cells were cultured in DMEM (Gibco, USA) supplemented with 10% heat-inactivated FBS, penicillin (100 U/mL), and streptomycin sulfate (100 μg/mL). The THP-1 human monocytic cell line was cultured in RPMI 1640 (Gibco, USA) supplemented with 10% heat-inactivated FBS, penicillin (100 U/mL), and streptomycin sulfate (100 μg/mL). All cells were maintained at 37°C with 5% CO₂.

Choudhury S.M., Ma X., Zeng Z., Luo Z., Li Y., Nian X., Ma Y., Shi Z., Song R., Zhu Z., Cao W., Pei J, & Zheng H. (2022). Senecavirus a 3D Interacts with NLRP3 to Induce IL-1β Production by Activating NF-κB and Ion Channel Signals. Microbiology Spectrum, 10(2), e02097-21.

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Cell Culture Conditions for HEK293T, PK15, and MDCK Cells

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Human embryonic kidney 293 T (HEK293 T) (ATCC® CRL-3216^™, Manassas, VA, USA), pig kidney (PK(15)) ((ATCC® CCL-33^™, Manassas, VA, USA) and Madin-Darby canine kidney (MDCK) (ATCC® CRL-2935^™, Manassas, VA, USA) cells were grown in Dulbecco’s modified Eagle’s medium (DMEM) (Sigma, St. Louis, MO) supplemented with 10 % fetal bovine serum (FBS) (Sigma, St. Louis, MO), 2 mM of L-glutamine (Sigma, St. Louis, MO) and 1 % of antibiotic/antimycotic solution containing 100 units/mL penicillin G, 0.1 mg/mL streptomycin sulfate, and 0.25 ug/mL amphotericin B (VWR, Radnor, PA)(DMEM/FBS). The cells were cultured at 37 °C under 5 % CO₂. Opti-MEM (Life Technologies, Carlsbad, CA)) supplemented with 1 % of antibiotic/antimycotic solution (Opti-MEM/AB) was used during transfections and virus rescue as indicated below.

Seibert B., Angel M., Caceres C.J., Sutton T., Kumar A., Ferreri L., Cardenas-Garcia S., Geiger G., Rajao D, & Perez D.R. (2020). Development of a swine RNA polymerase I driven Influenza reverse genetics system for the rescue of type A and B Influenza viruses. Journal of virological methods, 288, 114011.

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Cell Culture Protocols for Multiple Cell Lines

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Porcine fetal fibroblasts (PFF) cells (Chinese academy of sciences, Guangzhou, Guangdong province, China) were maintained in DMEM (C11995500BT, Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 15% Fetal Bovine Serum (FBS) (26010074, Thermo Fisher Scientific, Waltham, MA, USA), 1% penicillin/streptomycin (15140122, Thermo Fisher Scientific, Waltham, MA, USA), 1% non-essential amino acids (11140050, Thermo Fisher Scientific, Waltham, MA, USA), and 1% GlutaMAX (35050061, Thermo Fisher Scientific, Waltham, MA, USA); PK15 cells (CCL-33, ATCC, Manassas, VA, USA) were maintained in MEM (C11095500BT, Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% FBS and 1% penicillin/streptomycin. CHO-K1 (CCL-61, ATCC, Manassas, VA, USA) cells were cultured in DMEM/F-12 (11320033, Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% FBS, 100 U/mL penicillin and 100 μg/mL streptomycin; HepaRG cells (MMHPR116, Millpore, Burlington, MA, USA) were cultured in William’s E Medium (12551032, Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% FBS, and 1% penicillin/streptomycin; HepG2 (HB-8065, ATCC, Manassas, VA, USA) and HEK293T (CRL-3216, ATCC, Manassas, VA, USA) were maintained in DMEM supplemented with 10% FBS, and 1% penicillin/streptomycin. All cell lines were maintained at 37 °C in a humidified incubator with 5% CO₂.

Xu W., Zuo Q., Feng D., He C., Lin C., Huang D., Wan Y., Chen F., Mo G., Sun Q., Du H, & Huang L. (2022). Validation Study to Determine the Accuracy of Widespread Promoterless EGFP Reporter at Assessing CRISPR/Cas9-Mediated Homology Directed Repair. Current Issues in Molecular Biology, 44(4), 1688-1700.

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Maintenance of Cell Lines from Multiple Species

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Human Embryo Kidney (HEK) 293T (ATCC: CRL-11268), Madin Darby Canine Kidney Cells (MDCK, ATCC® CCL-34™) and Swine kidney epithelial cells (PK15, ATCC® CCL-33®) were maintained as suggested by the manufacturer's instructions. Equine Adipose-Derived Stromal Cells (EADSC), Bovine Bone Marrow Stromal Cells (BBMSC), and Alpaca derived Skin Stromal cells (ADSSC) were derived, immortalized, and maintained as previously described (22 (link)–24 (link)). All cell lines were cultured in Eagle's Minimal Essential Medium (EMEM, Gibco) containing 10% fetal bovine serum (FBS), 2 mM of L-glutamine (Gibco), 100 IU/mL of penicillin (Gibco), 100 μg/mL of streptomycin (SIGMA), and 0.25 μg/mL of amphotericin B (Gibco) and were incubated at 37°C/5% CO₂ in a humidified incubator.

Bastos R.G., Franceschi V., Tebaldi G., Connelley T., Morrison W.I., Knowles D.P., Donofrio G, & Fry L.M. (2019). Molecular and Antigenic Properties of Mammalian Cell-Expressed Theileria parva Antigen Tp9. Frontiers in Immunology, 10, 897.

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SARS-CoV-2 Propagation in Swine Cell Lines

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SARS-CoV-2 USA-WA1/2020 isolate (GenBank accession # MN985325) [34 (link)] was obtained from BEI resources (catalog # NR-52281, American Type Culture Collection [ATCC®]. Manassas, VA, USA). The virus was passaged three times in VeroE6 cells (ATCC® CRL-1586™), before being passaged two times in swine testicle (ST; ATCC CRL-1746™) and four times in porcine kidney (PK-15; ATCC® CCL-33™) cell lines to investigate suitability of these swine cell lines for propagation of SARS-CoV-2. The first passage of the SARS-CoV-2 virus from ST cells was used to prepare the challenge material. The titre of the virus inoculum stock was 2.5 × 10⁵ TCID₅₀/mL All experiments involving the SARS-CoV-2 virus were performed under Biosafety Level (BSL) 3+ conditions at the Biosecurity Research Institute (BRI) at Kansas State University (KSU), Manhattan, KS, USA.

Meekins D.A., Morozov I., Trujillo J.D., Gaudreault N.N., Bold D., Carossino M., Artiaga B.L., Indran S.V., Kwon T., Balaraman V., Madden D.W., Feldmann H., Henningson J., Ma W., Balasuriya U.B, & Richt J.A. (2020). Susceptibility of swine cells and domestic pigs to SARS-CoV-2. Emerging Microbes & Infections, 9(1), 2278-2288.

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Propagation of Porcine Circovirus Strains

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SP2/0 cells (ATCC® CRL-1581™) and PK15 cells (ATCC® CCL-33™) free of PCV1 and PCV2 were grown in Dulbecco’s modified eagle medium (DMEM, Invitrogen, Carlsbad, CA, USA) containing 10% heat-inactivated fetal bovine serum (FBS, Gibco, Grand Island, NY, USA), 100 U/mL penicillin, and 0.1 mg/mL streptomycin for mAb preparation and virus propagation. Different PCV2 strains and one PCV1/G strain were used in this study. Strain origins, genotypes, and accession numbers are shown in Table 1. The experimental animals used for mAb preparation were 6-week-old female BALB/c mice provided by the Weitong Lihua Laboratory Animal Technology Co., Ltd. (Beijing, China). MAb 5F2 (Huang et al. 2011 (link)), mAb 3A5 (Huang et al. 2019 (link)), and PCV2 polyclonal antibody (pAb) (Huang et al. 2019 (link)) were used as controls.Table 1

Origins of the PCV2 strains used in this study

Isolates name	Isolate region	Isolate time	Genotype	Genoome length (nt)	Accession numbers
LG	Jilin	2008	PCV2a	1768	HM038034
CL	Jilin	2007	PCV2a	1768	HM038033
MDJ	Heilongjiang	2019	PCV2b	1767	OL452025
BY	Heilongjiang	2020	PCV2b	1767	OL452026
LN590516	Liaoning	2016	PCV2b	1767	MK347352
HLJHH	Heilongjiang	2019	PCV2d	1767	OL452028
SDRS	Shandong	2019	PCV2d	1767	OL452024
LNHC	Liaoning	2019	PCV2d	1767	OL452027

Yu C., Wei Y., Zhang H., Liu J., Feng L., Liu C, & Huang L. (2022). Rapid detection of porcine circovirus type 2 by a red latex microsphere immunochromatographic strip. Applied Microbiology and Biotechnology, 106(17), 5757-5769.

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Culturing BHK-21 and PK-15 Cells for FMDV Propagation

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Baby hamster kidney cells [BHK-21, American Type Culture Collection (ATCC) CCL-10] and porcine kidney cells (PK-15, ATCC CCL-33) were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Gibco, CA, USA) containing 10% fetal bovine serum (FBS; Gibco), 100 U/ml penicillin, and 100 μg/ml streptomycin and cultured in an incubator at 37°C under 5% CO₂. FMDV strain O/BY/CHA/2010 (GenBank accession no. JN998085.1) is stored by the OIE/National Foot-and-Mouth Disease Reference Laboratory (Lanzhou, China). FMDV was grown and passaged in BHK-21 cells, and the titer of FMDV was determined with a median tissue culture infective dose (TCID₅₀) assay.

Guan J., Han S., Wu J., Zhang Y., Bai M., Abdullah S.W., Sun S, & Guo H. (2021). Ribosomal Protein L13 Participates in Innate Immune Response Induced by Foot-and-Mouth Disease Virus. Frontiers in Immunology, 12, 616402.

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Cell Culture Conditions for HEK293T, PK15, and MDCK Cells

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