Log In Sign up
The largest database of trusted experimental protocols

7 8 dhf

Manufactured by Merck Group
Visit Supplier
Sourced in United States

7,8-DHF is a chemical compound used as a laboratory reagent. It functions as a reference standard or control substance for analytical and research purposes. The detailed technical specifications and intended applications of this product are not provided, as an unbiased and purely factual description would require more information than is currently available.

Automatically generated - may contain errors

Lab products found in correlation

Dimethyl sulfoxide (dmso), by Merck Group (4 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (4 mentions) Ana 12, by Merck Group (2 mentions) Platinumtaq master mix, by Thermo Fisher Scientific (2 mentions) Neurobasal medium, by Thermo Fisher Scientific (2 mentions) B27 medium, by Thermo Fisher Scientific (2 mentions) Vectashield mounting media with dapi, by Vector Laboratories (2 mentions) Trypsin edta, by Thermo Fisher Scientific (2 mentions) Hanks balanced salt solution (hbss), by Thermo Fisher Scientific (2 mentions) Anti nrf2, by Merck Group (1 mentions) Nuclear protein extraction kit, by Beyotime (1 mentions) Cell counting kit 8 cck 8, by Beyotime (1 mentions) Vb 037, by Enamine (1 mentions) Congo red, by Merck Group (1 mentions) Kaempferol, by Merck Group (1 mentions) Corn oil, by Merck Group (1 mentions) 5 ethynyl 20 deoxyuridine edu, by Merck Group (1 mentions) Tamoxifen, by Merck Group (1 mentions) Brain derived neurotrophic factor (bdnf), by Merck Group (1 mentions) Apigenin, by Merck Group (1 mentions)

Close spelling variants of this product

7,8-dihydroxyflavone (DHF) 7,8-Dihydroxyflavone (7,8-DHF)

24 protocols using 7 8 dhf

1

BMSC Treatment with 7,8-DHF and Inhibitor

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
The compound 7, 8-DHF was purchased from Sigma (⩾98%). 7, 8-DHF was dissolved in PBS with 17% dimethyl sulfoxide (DMSO). BMSCs were seeded into 6-well culture plates at a density of (2 × 107 cells/well) and cultured to ~90% confluence. BMSCs were treated with 7, 8-DHF using an optimal concentration of 500 nMol/L, as determined in a previous study, for 72 h.24 (link)
 Fresh culture medium was replaced, and the BMSCs were separated into four experimental groups: (1) a negative control (NC) group, treated with medium only, (2) cells treated with 7, 8-DHF, (3) 7, 8-DHF + U0126 group, the cells were treated with 7, 8-DHF and U0126 (selective inhibitor of ERK1/2 signaling, 5 μM, Sigma-Aldrich, St. Louis, MO, USA), and (4) BDNF group, the cells were treated with human recombinant BDNF (159 nMol/L, Sigma).18 (link)
 Following the treatment period, protein was isolated from the BMSCs for ELISA and Western blot analysis.
Li W.Y., Jia H., Wang Z.D., Zhai F.G., Sun G.D., Ma D., Liu G.B., Li C.M, & Wang Y. (2020). Combinatory transplantation of mesenchymal stem cells with flavonoid small molecule in acellular nerve graft promotes sciatic nerve regeneration. Journal of Tissue Engineering, 11, 2041731420980136.
+ Open protocol
+ Expand
2

Tamoxifen-induced Myrf inactivation in OPCs

Check if the same lab product or an alternative is used in the 5 most similar protocols
Tamoxifen (300 mg/kg; Sigma-Aldrich) was administered at approximately postnatal day 70 by gavage for 4 d to induce the inactivation of Myrf in OPCs. Tamoxifen was prepared fresh on the day of administration by diluting it with corn oil (Sigma-Aldrich) to a concentration of 40 mg/ml as previously outlined (McKenzie et al., 2014 (link)). The mice were given at least 3 weeks to recover from any side effects of the Tamoxifen, such as weight loss, before behavioral testing. 7,8-DHF (Sigma-Aldrich) was dissolved in 17% DMSO in PBS. MyRFDHF mice received one intraperitoneal (IP) injection of 7,8-DHF 5 mg/kg, immediately following each MWM training session (seven in total), while MyRF−/− and MyRF+/− mice received intraperitoneal injections of vehicle (17% DMSO in PBS). Care was taken to administer IP injections on alternating sides of the abdomen to limit sensitivity. The dose of 7,8-DHF has been widely used and shown to improve symptoms in a number of disease models (Zeng et al., 2012 (link); Zhang et al., 2014 (link); Stagni et al., 2017 (link)) 5-Ethynyl-20-deoxyuridine (EdU; Sigma-Aldrich) was administered to the mice via drinking water at a concentration of 0.2 mg/ml for 4 d starting on the last day of training.
Rawlings-Mortimer F, & Johansen-Berg H. (2023). Rescue of Long-Term Spatial Memory by 7,8-Dihydroxyflavone in Mice with Reduced Oligodendrogenesis. eNeuro, 10(5), ENEURO.0498-22.2023.
+ Open protocol
+ Expand
3

Nrf2 and HO-1 Signaling Pathway Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
7,8-DHF (purity >99%) was procured from Sigma-Aldrich; Merck KGaA. Anti-Nrf2 (cat. no. BS1258) and anti-HO-1 (cat. no. BS6626) antibodies were supplied by Bioworld Technology at a dilution of 1:1,000. Anti-actin (cat. no. 4970) and anti-lamin B (cat. no. 13435) antibodies were obtained from Cell Signaling Technology, Inc. at a dilution of 1:5,000. Secondary HRP-conjugated goat anti-rabbit (cat. no. BS13278) antibody was purchased from Bioworld Technology, Inc. and used at a dilution of 1:5,000. The Cell Counting Kit-8 (CCK-8) and nuclear protein extraction kit were purchased from Beyotime Institute of Biotechnology. The superoxidase dismutase (SOD) and malondialdehyde (MDA) assay kits were supplied by Nanjing Jiancheng Bioengineering Institute. The fluorescent probe 2′,7′-dichlorodihydrofluorescein diacetate (H2DCFDA) was obtained from Molecular Probes; Thermo Fisher Scientific, Inc.
Cai D., Feng W., Liu J., Jiang L., Chen S., Yuan T., Yu C., Xie H., Geng D, & Qin J. (2019). 7,8-Dihydroxyflavone activates Nrf2/HO-1 signaling pathways and protects against osteoarthritis. Experimental and Therapeutic Medicine, 18(3), 1677-1684.
+ Open protocol
+ Expand
4

Diverse Bioactive Compound Synthesis

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
One flavone ZN-006 [2-((5-hydroxy-4-oxo-2-phenyl-4H-chromen-7-yl)oxy)acetic acid], one benzofuran ZN-013 [(4-methoxybenzofuran-2-yl)(phenyl)methanone], and four quinolines VB-030 [2-(pyridin-4-yl)-4-(p-tolyl)quinoline], VB-031 [(E)-3-(3-(1H-benzo[d]imidazol-2-yl)acryloyl)-6-methyl-4-phenylquinolin-2(1H)-one], VB-037 [(E)-4-(3-(2-(5-nitroquinolin-2-yl)vinyl)quinolin-2-yl)morpholine] and VB-041 [2-(pyridin-4-yl)-N-(3-(N-(3,4,5,6-tetrahydro-2H-azepin-7-yl)sulfamoyl)phenyl)quinoline-4-carboxamide] were purchased from Enamine (Kyiv, Ukraine). Procedures for producing coumarins ZN-014 (ethyl 5-hydroxy-2-oxo- 2H-chromene-3-carboxylate) and ZN-015 [(E)-4-hydroxy-3-(3-(2-hydroxyphenyl)acryloyl)-2H-chromen-2-one] were as stated [28 (link)]. Congo red, kaempferol and 7,8-DHF, controls for cellular and/or biochemical assays were obtained from Sigma-Aldrich (St. Louis, MO, USA). In addition, LM-031 (3-benzoyl-5-hydroxychromen-2-one), a control for evaluating TRKB signaling, was synthesized as stated [30 (link)].
Weng Z.K., Lin T.H., Chang K.H., Chiu Y.J., Lin C.H., Tseng P.H., Sun Y.C., Lin W., Lee-Chen G.J, & Chen C.M. (2023). Using ΔK280 TauRD Folding Reporter Cells to Screen TRKB Agonists as Alzheimer’s Disease Treatment Strategy. Biomolecules, 13(2), 219.
+ Open protocol
+ Expand
5

Modulating trkB Signaling in Adolescent Mice

Cited 2 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
Male and female mice were administered (i.p.) the putative trkB agonist, 7,8-dihydroxyflavone (7,8-DHF; Sigma; 3 mg/kg; dissolved in 17% DMSO and saline; Zhang et al., 2014 (link)), or vehicle daily from P39 to 47, overlapping with the end of the adolescent CORT exposure period. This period is marked by significant pruning of dendritic spines on excitatory pyramidal neurons in the mouse orbital PFC (oPFC; Shapiro et al., 2017b (link)). Additionally, expression levels of trkB in the oPFC increase during this time (Shapiro et al., 2017b (link)), potentially facilitating activity-dependent refinement of synaptic connections and stabilizing synapses that are not pruned. This period was also determined based on prior work (Barfield et al., in press ).
The trkB antagonist, ANA-12 (Sigma; 0.5 mg/kg, 1% DMSO), or vehicle was administered (i.p.) daily from P31 to 42, to match the period of adolescent CORT exposure.
Barfield E.T, & Gourley S.L. (2017). Adolescent Corticosterone and TrkB Pharmaco-Manipulations Sex-Dependently Impact Instrumental Reversal Learning Later in Life. Frontiers in Behavioral Neuroscience, 11, 237.
+ Open protocol
+ Expand
6

Intraperitoneal 7,8-DHF Administration

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
7,8-Dihydroxyflavone (7,8-DHF) was intraperitoneally administered once a day for three consecutive days at a dose of 10 mg/kg. The dose was chosen on the basis of a dose test in this study and a previous study [32 (link)]. 7,8-DHF was purchased from Sigma Aldrich (St. Louis, MO, USA).
Lee Y, & Han P.L. (2019). Early-Life Stress in D2 Heterozygous Mice Promotes Autistic-like Behaviors through the Downregulation of the BDNF-TrkB Pathway in the Dorsal Striatum. Experimental Neurobiology, 28(3), 337-351.
+ Open protocol
+ Expand
7

BDNF and 7,8-DHF Neuroprotective Effects

Check if the same lab product or an alternative is used in the 5 most similar protocols
All drugs, such as BDNF, 7,8-DHF, K252a, and dimethyl sulfoxide (DMSO), were purchased from Sigma-Aldrich (Harbin, China). Ethanol solutions were prepared from 99% (v/v) ethyl alcohol (Oceanpak, Shenzhen, China) using tap water. 7,8-DHF was diluted with DMSO, and administered through intraperitoneal injection. BDNF or K252a dissolved in artificial cerebrospinal fluid were microinjected into VTA.
Li X.X., Yang T., Wang N., Zhang L.L., Liu X., Xu Y.M., Gao Q., Zhu X.F, & Guan Y.Z. (2020). 7,8-Dihydroxyflavone Attenuates Alcohol-Related Behavior in Rat Models of Alcohol Consumption via TrkB in the Ventral Tegmental Area. Frontiers in Neuroscience, 14, 467.
+ Open protocol
+ Expand
8

Alzheimer's Aβ Folding Reporter Assay

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Three flavones, 7,8-DHF, quercetin (3,3’,4’,5,7-pentahydroxyflavone) and apigenin (4’,5,7-trihydroxyflavone) (Sigma-Aldrich, St. Louis, MO, USA), were tested. The three flavones demonstrated solubility up to 100 μM in cell culture medium. A single copy of the Aβ-green fluorescent protein (GFP) gene was integrated into SH-SY5Y cells (ATCC No. CRL-2266) to create human Aβ folding reporter SH-SY5Y cells (Chang et al., 2016 (link)). The expression of Aβ-GFP RNA increased 28-30 folds with the addition of 5 µg/mL of doxycycline (Sigma-Aldrich) (Huang et al., 2021 (link)).
Chiu Y.J., Teng Y.S., Chen C.M., Sun Y.C., Hsieh-Li H.M., Chang K.H, & Lee-Chen G.J. (2023). A Neuroprotective Action of Quercetin and Apigenin through Inhibiting Aggregation of Aβ and Activation of TRKB Signaling in a Cellular Experiment. Biomolecules & Therapeutics, 31(3), 285-297.
+ Open protocol
+ Expand
9

Evaluation of 7,8-Dihydroxyflavone Effects

Check if the same lab product or an alternative is used in the 5 most similar protocols
7,8-DHF was purchased from Sigma-Aldrich (St. Louis, MO, USA) and dissolved in dimethyl sulfoxide (DMSO, Sigma-Aldrich). Dulbecco’s modified Eagle medium (DMEM), fetal bovine serum (FBS), bovine calf serum (BCS), penicillin-streptomycin, and 2-NBDG were purchased from Invitrogen (Carlsbad, CA, USA). The 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxy-methoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, Inner Salt (MTS) assay kit was obtained from Promega (Madison, WI, USA). The enzyme-linked immunosorbent assay (ELISA) kits for TNF-α, IL-6, MCP-1, and adiponectin were obtained from R&D Systems (Minneapolis, MN, USA). Nuclear and cytoplasmic extraction reagents and the assay kit for NEFA were provided by Thermo Fisher Scientific (Rockford, IL, USA) and FUJIFILM Wako Pure Chemical Corporation (Chuo-Ku, Osaka, Japan), respectively. Antibodies specific for phospho-c-Jun N-terminal kinases (p-JNK), total-JNK (t-JNK), phospho-Akt (Ser473), Akt, and NF-κB were purchased from Cell Signaling Technology (Danvers, MA, USA). The antibody for lamin B was purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA), and antibodies for α-tubulin and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) were from Ab Frontier (Seoul, Korea). All other chemicals were obtained from Sigma-Aldrich.
Shin Y.E., Choi J.W., Park Y.I, & Kim H.K. (2023). 7,8-Dihydroxyflavone Attenuates Inflammatory Response and Insulin Resistance Induced by the Paracrine Interaction between Adipocytes and Macrophages. International Journal of Molecular Sciences, 24(4), 3520.
+ Open protocol
+ Expand
10

Intranasal and Stereotaxic Delivery of 7,8-DHF and ANA-12

Cited 3 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
For intraperitoneal administration (i.p.), 7,8-dihydroxyflavone (7,8-DHF; Catalog number: D5446, Sigma-Aldrich, USA, 5 mg/kg) was prepared in vehicle of 20% β-cyclodextrin in normal saline, and ANA-12 (Catalog number: HY-12497, MedChem Express, USA, 0.5 mg/kg) was prepared in vehicle of 20% β-cyclodextrin in normal saline. The doses of 7,8-DHF (5 mg/kg), and ANA-12 (0.5 mg/kg) were selected as reported previously [31 (link), 46 (link), 47 (link)].
For stereotaxic drug microinjection, Recombinant Human BDNF (rhBDNF; Catalog number: AF-450-02, Peprotech, USA, 0.5 μg/μl, 0.5 μl/site) was prepared in vehicle of 0.1% BSA, 7,8-DHF (1 μg/μl, 0.5 μl/site), and ANA-12 (1 μg/μl, 0.5 μl/site) was prepared in vehicle of 20% β-cyclodextrin in normal saline. The dose of 7,8-DHF was chosen based on our pilot study. The doses of BDNF [48 (link)], and ANA-12 [49 (link)] were selected as reported previously.
Sun Y.X., Su Y.A., Wang Q., Zheng J.Y., Zhang C.C., Wang T., Liu X., Ma Y.N., Li X.X., Zhang X.Q., Xie X.M., Wang X.D., Li J.T, & Si T.M. (2023). The causal involvement of the BDNF-TrkB pathway in dentate gyrus in early-life stress-induced cognitive deficits in male mice. Translational Psychiatry, 13, 173.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!