Log In Sign up
The largest database of trusted experimental protocols

Diazinon

Manufactured by Merck Group
Visit Supplier
Sourced in United States, Germany

Diazinon is a laboratory equipment product manufactured by Merck Group. It is a colorless to pale yellow liquid used for various analytical and research applications. Diazinon is a pesticide that can be used as a standard or reference material in chemical analysis and testing.

Automatically generated - may contain errors

Lab products found in correlation

Malathion, by Merck Group (13 mentions) Dimethoate, by Merck Group (11 mentions) Fenitrothion, by Merck Group (8 mentions) Chlorpyrifos, by Merck Group (7 mentions) Atrazine, by Merck Group (6 mentions) Methyl parathion, by Merck Group (5 mentions) Parathion, by Merck Group (4 mentions) Carbaryl, by Merck Group (4 mentions) Permethrin, by Merck Group (4 mentions) Acephate, by Merck Group (4 mentions) Lindane, by Merck Group (4 mentions) Quinalphos, by Merck Group (4 mentions) Maneb, by Merck Group (3 mentions) Bovine serum albumin (bsa), by Merck Group (3 mentions) Methanol, by Merck Group (3 mentions) Deltamethrin, by Merck Group (3 mentions) Carbofuran, by Merck Group (3 mentions) Dichlorvos, by Merck Group (3 mentions) Ethion, by Merck Group (3 mentions) Imazalil, by Merck Group (2 mentions)

39 protocols using diazinon

1

Quantification of Insecticide-Induced AChE Inhibition

Check if the same lab product or an alternative is used in the 5 most similar protocols
Chemical standards of malathion, methidathion, diazinon, dimethoate, and their respective oxons (Fig. S1) were purchased from Fujifilm Wako Pure Chemical Corporation (Osaka, Japan) and were used as received. To avoid any effect of organic solvents on the experimental results, stock solutions of the insecticides (malathion, 29 μM; methidathion, 230 μM; diazinon, 38 μM; dimethoate, 30 μM) and oxons (malathion-oxon, diazinon-oxon, and dimethoate-oxon, 100 μM; methidathion-oxon, 1000 μM) were prepared in a Milli-Q water-based (Milli-Q Advantage, Millipore Co., Bedford, MA, USA) phosphate buffer (10 mM, pH 7.0). AChE (derived from human erythrocytes) was purchased from Merck KGaA (Darmstadt, Germany). ACh and choline (Ch) were purchased from Fujifilm Wako Pure Chemical Corporation.
Matsushita T., Fujita Y., Omori K., Huang Y., Matsui Y, & Shirasaki N. (2020). Effect of chlorination on anti-acetylcholinesterase activity of organophosphorus insecticide solutions and contributions of the parent insecticides and their oxons to the activity. Chemosphere, 261.
+ Open protocol
+ Expand
2

Pesticide Exposure Effects on BM-MSCs

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Three doses of pesticides were used. The first was the high nutritional daily intake (hNDI), calculated for the French population of all ages and corresponding to the lowest doses of pesticides used in our study. The second was the ADI, which is the threshold of safety in humans for lifetime exposure. The last was a value of three times the ADI (3ADI).
BM-MSCs were amplified as previously described [20 (link)]. Briefly, they were cultured in 150 cm2 flasks (Corning® cell culture flask, Sigma-Aldrich, St. Louis, MO, USA) for 3 weeks (37 °C, 5% CO2) in α-MEM supplemented with FGF2 (1 ng/mL, R&D system, Minneapolis, MN, USA) and were tested at passages 2 to 4 (2000–4000 cells/cm2). This medium, supplemented with pesticides (hNDI, ADI and 3ADI), was renewed every 3 days. The doses of pesticides used (chlorpyrifos ethyl, dimethoate, diazinon, iprodione, imazalil, maneb and mancozeb; Sigma-Aldrich) were extrapolated, for each pesticide, from 3 values according to previous studies (Table S2) [9 (link),10 (link)].
Foucault A., Ravalet N., Besombes J., Picou F., Gallay N., Babin L., Bourgeais J., Hamard S., Domenech J., Loyer P., Vallet N., Lejeune J., Gyan E., Béné M.C., Vallette F., Olivier C, & Hérault O. (2021). Low-Dose Pesticides Alter Primary Human Bone Marrow Mesenchymal Stem/Stromal Cells through ALDH2 Inhibition. Cancers, 13(22), 5699.
+ Open protocol
+ Expand
3

Diazinon Chemical Preparation

Check if the same lab product or an alternative is used in the 5 most similar protocols
All chemicals used in the experiments were reagent grade. All solutions were prepared with distilled water. Diazinon was obtained from Sigma-Aldrich.
Ehrampoush M.H., Sadeghi A., Ghaneian M.T, & Bonyadi Z. (2017). Optimization of diazinon biodegradation from aqueous solutions by Saccharomyces cerevisiae using response surface methodology. AMB Express, 7, 68.
+ Open protocol
+ Expand
4

Sensitive Parathion Detection Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
Parathion (45607) was purchased from Sigma-Aldrich and anti-Parathion (ABIN113883) polyclonal antibodies was purchased as rabbit sera from Antibodies-online.com. Au-NPs 10nm OD20 were purchased from InnovaCoat®. The type G immunoglobulins (IgG) were purified using the Protein A Antibody Purification Kit (PURE1A) from Sigma-Aldrich. 5,5′-dithiobis-(2-nitrobenzoic acid) also known as Ellman’s reagent (D8130), Bovine serum albumin (A2153) and the compounds used for the specificity tests, p-nonylphenol (46018), dichlorvos (45441), diazinon (45428) and paraoxon (36186), were from Sigma-Aldrich. The pollutant samples were prepared using PBS 1x buffer solution in the fume hood. MilliQ water, sulfuric acid 98% and hydrogen peroxide 40% were used for the cleaning procedure of the QCM gold surfaces.
Della Ventura B., Iannaccone M., Funari R., Pica Ciamarra M., Altucci C., Capparelli R., Roperto S, & Velotta R. (2017). Effective antibodies immobilization and functionalized nanoparticles in a quartz-crystal microbalance-based immunosensor for the detection of parathion. PLoS ONE, 12(2), e0171754.
+ Open protocol
+ Expand
5

Synthesis of Gold Nanoparticles

Check if the same lab product or an alternative is used in the 5 most similar protocols
Gold(III) chloride trihydrate
(HAuCl4·3H2O), iprobenfos, edifenphos,
diazinon, and tebuconazole were obtained from Sigma-Aldrich (St. Louis,
MO). Sodium sulfate anhydrous was manufactured by Jin Chemical (Bucheon,
Korea). Imidazole and trisodium citrate dihydrate were purchased from
Bio-Basic (Ontario, Canada). Methanol was purchased from Merck Chemicals
(Darmstadt, Germany).
Baek S.H., Lee S.W., Kim E.J., Shin D.H., Lee S.W, & Park T.J. (2017). Portable Agrichemical Detection System for Enhancing the Safety of Agricultural Products Using Aggregation of Gold Nanoparticles. ACS Omega, 2(3), 988-993.
+ Open protocol
+ Expand
6

Planarian Exposure to Agrochemicals

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
For each experiment, at least 30 adult planarians were exposed to either IO water or 100 µM of either glyphosate, RR, or RC. This concentration was chosen as it was the highest concentration that was sublethal for all three chemical formulations. As an assay positive control for AChE inhibition, planarians were exposed to either 0.178 µM diazinon (CAS # 333-41–5, Sigma-Aldrich) in 0.5% dimethyl sulfoxide (DMSO, Sigma-Aldrich) or to 0.5% DMSO (solvent control). This concentration of diazinon has robustly caused significant planarian AChE inhibition in our laboratory (Ireland et al, 2022 (link)). Planarians were exposed in tissue culture-treated 12-well plates, with 6 planarians in 1.2 mL of the test solution per well, thus keeping the ratio of chemical/planarian consistent with the HTS set-up. Any fission events or planarians from wells with death were excluded from the assay.
Fuselier S.G., Ireland D., Fu N., Rabeler C, & Collins E.M. (2023). Comparative toxicity assessment of glyphosate and two commercial formulations in the planarian Dugesia japonica. Frontiers in Toxicology, 5, 1200881.
+ Open protocol
+ Expand
7

Immobilized Lipase Enzyme Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
All reagents used in this study were purchased from Sigma Aldrich (Saint Quentin Fallavier) France, including, Lipase from Candida Rugosa enzyme (CRL, type VII, ≥700 unit/mg solid), Lipase from porcine pancreas (PPL, type II, 30–90 units/mg protein), bovine serum albumin (BSA), diazinon, parathion methyl, paraoxon methyl, atrazine, sevin, simazine, fenitrothion, sodium phosphate dibasic, sodium phosphate monobasic, KH2PO4, K2HPO4, glutaraldehyde (grade II, 25% aqueous solution), glycerol (≥99%), 6-methyl-5propyl-4 pyrimidinone, N-hydroxysuccinimide (NHS), 1-ethyl-3(3-(dimethyl-amino)propyl)carbodiimide (EDC), acidthiol(16mercacaptohexadecanoicacid). Sulfuric acid (96%), hydrogen peroxide (30%), ethanol (99%) was purchased from Fluka. All solutions were made up with ultrapure water (resistivity no less than 18 MΩ cm and obtained from a Millipore purification system).
Zehani N., Dzyadevych S.V., Kherrat R, & Jaffrezic-Renault N.J. (2014). Sensitive impedimetric biosensor for direct detection of diazinon based on lipases. Frontiers in Chemistry, 2, 44.
+ Open protocol
+ Expand
8

Aptamer-based Biosensor for Pesticide Detection

Check if the same lab product or an alternative is used in the 5 most similar protocols
Chloroauric acid tetrahydrate (HAuCl4·4H2O), trisodium citrate, sodium citrate dihydrate, Triton X-100, mycose, sodium dodecyl sulfonate (SDS), NaCl, Na3PO4·12H2O, Tween-20, sucrose, deoxyadenosine triphosphate (dATP), bovine serum albumin (BSA), phosphate buffered saline (PBS, pH 7.4, 0.01 M), sodium chloride-sodium citrate (SSC) buffer (20 concentrate, pH 7.0), borate buffer (BB, pH 9.0, 0.1 M), and the pesticides used in this study (chlopyrifos, malathion, diazinon, atrazine, carbaryl, acetamiprid, and 2,4-D.) were purchased from Sigma Chemical Company (St. Louis, MO, USA). Streptavidin was purchased from Invitrogen (Carlsbad, CA, USA). QDs nanobeads premodified by polystyrene maleic-anhydride copolymer were provided by Shanghai Kundao Biotech Co., Ltd. (Shanghai, China). Backing cards (HF000MC100), glass fiber sample pads (CFSP001700), conjugation pads (GFCP000800), nitrocellulose membranes (135s), and absorbent pads (CFSP001700) were purchased from Millipore (Bedford, MA, USA). Sequences of aptamers for chlorpyrifos (Jiao et al., 2017 , 2016 ), aptamers for diazinon (Jokar et al., 2017 (link)), aptamers for malathion (Bala et al., 2016 (link)) and corresponding biotinylated complementary sequences (Table S1), were synthesized and purified by Sangon Biotech Co., Ltd. (Shanghai, China). Double distilled water (ddwater) was used in all experiments.
Cheng N., Song Y., Fu Q., Du D., Luo Y., Wang Y., Xu W, & Lin Y. (2018). Aptasensor based on fluorophore-quencher nano-pair and smartphone spectrum reader for on-site quantification of multi-pesticides. Biosensors & bioelectronics, 117, 75-83.
+ Open protocol
+ Expand
9

Ultrasensitive Pesticide Detection Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
TMB Liquid Substrate System, luminol, bovine serum albumin (BSA), KMnO4, K2PtCl4 (Pt, 44.99%), HAuCl4 (Au, 49.98%), Na2PdCl4 (Pd, 49.98%), ascorbic acid, PDDA, 1,3-diphenylisobenzofuran (DPBF), chlorpyrifos, parathion, methyl parathion, diazinon, malathion and fenitrothion were all purchased from Sigma-Aldrich (U.S.A.). Graphite, FeCl3, and K4[Fe(CN)6] were all provided by Alfa Aesar. Mouse monoclonal antibody for chlorpyrifos (antichlorpyrifos McAb) and chlorpyrifos–BSA bioconjugate were all obtained from Wuxi Determine Bio-Tech Co. Ltd. (China). Goat antimouse IgG was purchased from Abcam Inc. (USA). 40 nm gold nanoparticles were obtained from nanoComposix (U.S.A.). Nitrocellulose membrane, fiber sample pad, fiber conjugate pad and absorbent pad were purchased from Millipore Corp. (U.S.A.). Phosphate buffer saline (PBS, 0.10 M pH 7.4) containing 1.0% BSA and 0.05% Tween-20 was adopted as the blocking buffer for the pretreatment of the conjugate pad and absorbent pad. Polystyrene 96-well microplates were provided by Corning Incorporated (U.S.A.). Astragalus and Poria cocos were purchased from a local pharmacy in Chongqing (China) and water sample was collected from South Fork Palouse River outside of Washington State University. All other reagents of analytical grade were utilized as received without further treatment.
Ouyang H., Lu Q., Wang W., Song Y., Tu X., Zhu C., Smith J.N., Du D., Fu Z, & Lin Y. (2018). Dual-Readout Immunochromatographic Assay by Utilizing MnO2 Nanoflowers as the Unique Colorimetric/Chemiluminescent Probe. Analytical chemistry, 90(8), 5147-5152.
+ Open protocol
+ Expand
10

Acute Diazinon Exposure in Rats

Check if the same lab product or an alternative is used in the 5 most similar protocols
After a minimum 5 day acclimatisation period, rats received a single intraperitoneal (i.p.) injection to allow accurate and efficient delivery of Diazinon. In the cholinesterase activity study, rats received 0, 1.3, 13 or 39 mg/kg Diazinon and in the electrophysiology study rats received 0 or 39 mg/kg Diazinon. Doses were selected to cause <50% cholinesterase inactivation (below the threshold to induce overt hypercholinergic toxicity). Diazinon (Sigma–Aldrich, UK) was mixed with ethanol and Cremophor EL to make a suspension and diluted to the appropriate volume with 0.9% saline (final concentration 1% ethanol, 10% Cremophor EL) [4] . The Diazinon mixture or vehicle mixture (1% ethanol, 10% Cremophor EL, 89% saline) was administered shortly after preparation (1 ml/kg).
Judge S.J., Savy C.Y., Campbell M., Dodds R., Gomes L.K., Laws G., Watson A., Blain P.G., Morris C.M, & Gartside S.E. (2016). Mechanism for the acute effects of organophosphate pesticides on the adult 5-HT system. Chemico-Biological Interactions, 245, 82-89.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!