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2 protocols using acetyldaidzin

1

HPLC Analysis of Soy Isoflavones

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The HPLC-grade acetonitrile and water (Daejung Chemical & Metals Co., Siheung, Korea) were used as mobile phases for isoflavones analysis. Standards of isoflavone aglycones (daidzein, glycitein, and genistein) and β-glycosides (daidzin, glycitin, and genistin) were purchased from LC Laboratories (Woburn, MA, USA). Isoflavone acetylglycosides (acetyldaidzin, acetylglycitin, and acetylgenistin) were obtained from Nacalai tesque (Kyoto, Japan), and malonylglycosides (malonyldaidzin, malonylglycitin, and malonylgenistin) were obtained from GenDEPOT (Katy, TX, USA).
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2

Comprehensive Phytochemical Standards Database

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The standards for anthocyanins comprised delphinidin 3-O-glucoside chloride, petunidin 3-O-glucoside chloride, cyanidin chloride (Extrasynthese, Genay, France), and cyanidin 3-O-glucoside chloride (SinoStandards Bio-Tech Co., Ltd., Chengdu, China). The standards for flavan-3-ols consisted of catechin hydrate (Sigma Aldrich Co., St Louis, MO, USA) and epicatechin (Chemfaces, Wuhan, China). The standards for isoflavones comprised daidzein, glycitein, genistein, daidzin, glycitin, genistin (LC Laboratories, Woburn, MA, USA), acetyl daidzin, acetyl glycitin, acetyl genistin (Nacalai Tesque, Kyoto, Japan), malonyl daidzin, malonyl glycitin, and malonyl genistin (GenDEPOT, Katy, TX, USA).
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