Cells cultured in 35-mm glass-coated wells (Iwaki, Chiba, Japan) were fixed with cold acetone and ethanol (1:1) at −20 °C for 10 min. After rinsing in PBS, the cells were incubated with anti-LSR (1:100), anti-pYAP (1:100), anti-AMOT (1:100), and anti-Merlin (1:100) antibodies at room temperature for 1 h. Alexa Fluor 488 (green)-conjugated anti-rabbit IgG and Alexa Fluor 594 (red)-conjugated anti-mouse IgG (Invitrogen) were used as secondary antibodies. The specimens were examined using an epifluorescence microscope (Olympus, Tokyo, Japan) and a confocal laser scanning microscope (LSM5; Carl Zeiss, Jena, Germany).
Alexa fluor 488 green conjugated anti rabbit igg
Manufactured by Thermo Fisher Scientific
Sourced in Japan
Alexa Fluor 488 (green)-conjugated anti-rabbit IgG is a secondary antibody that binds to rabbit primary antibodies. It is used in various immunodetection techniques, such as immunofluorescence and Western blotting, to visualize the target proteins labeled with the primary rabbit antibody.
Automatically generated - may contain errors
Lab products found in correlation
Lsm 510, by Zeiss (10 mentions)
35 mm glass coated wells, by Iwaki (9 mentions)
Alexa fluor 594 red conjugated anti mouse igg, by Thermo Fisher Scientific (5 mentions)
Alexa fluor 592 red conjugated anti mouse igg, by Thermo Fisher Scientific (5 mentions)
Ix71 inverted microscope, by Olympus (5 mentions)
Epifluorescence microscope, by Olympus (3 mentions)
Plan apochromat, by Zeiss (1 mentions)
Confocor 3 lsm 510 meta, by Zeiss (1 mentions)
Lsm 410, by Zeiss (1 mentions)
12 protocols using alexa fluor 488 green conjugated anti rabbit igg
1
Immunofluorescence Analysis of Cytoskeletal Proteins
2
Immunofluorescence Imaging of Tight Junction Proteins
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The cultured cells in 35-mm glass-coated wells (Iwaki, Chiba, Japan) were fixed with cold acetone and ethanol (1:1) at −20°C for 10 min. After rinsing in PBS, the cells were incubated with anti-cytokeratin 7 (1:200), anti-LSR (1:100), anti-tricellulin (1:100), and anti-vimentin (1:100) antibodies at room temperature for 1 h. Alexa Fluor 488 (green)-conjugated anti-rabbit IgG and Alexa Fluor 594 (red)-conjugated anti-mouse IgG (Invitrogen) were used as secondary antibodies. The specimens were examined using an epifluorescence microscope (Olympus, Tokyo, Japan) and a confocal laser scanning microscope (LSM5; Carl Zeiss, Jena, Germany). Some images were captured using a Zeiss Elyra PS1 SIM equipped with a Zeiss Plan Apochromat inverted 63x/1.40 oil immersion objective lens using an Andor EM-CCD iXon 885 camera and a 1.6 x tube lens at room temperature (Carl Zeiss, Jena, Germany).
3
Immunofluorescence Analysis of Cytoskeletal Markers
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cells cultured in a 35-mm glass-coated dish (Iwaki, Chiba, Japan) were fixed with cold acetone and ethanol (1:1) at -20°C for 10 min, rinsed in PBS, and then incubated with anti-acetylated tubulin (1:400), anti-TRIC (1:200), anti-LSR (1:200), or anti-ZO-1 (1:200) at room temperature for 1 h. Alexa Fluor 488 (green)-conjugated anti-rabbit IgG and Alexa Fluor 594 (red)-conjugated anti-mouse IgG (Invitrogen) were used as secondary antibodies. Specimens were examined with either an epifluorescence (Olympus, Tokyo, Japan) or confocal laser scanning microscope (LSM5; Carl Zeiss, Jena, Germany).
4
Immunofluorescent Localization of Cell Junctional Proteins
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
A549 cells and HLE cells in 35-mm glass-coated wells (Iwaki, Chiba, Japan), were fixed with cold acetone and ethanol (1:1) at –20°C for 10 min. After rinsing in PBS, the cells were incubated with anti-LSR, anti-CLDN-2, anti-OCLN, anti-p63, and anti-CK7 antibodies (1:100) overnight at 4°C. Alexa Fluor 488 (green)-conjugated anti-rabbit IgG and Alexa Fluor 592 (red)-conjugated anti-mouse IgG (Invitrogen) were used as secondary antibodies. The specimens were examined and photographed with an Olympus IX 71 inverted microscope (Olympus Corp, Tokyo, Japan) and a confocal laser scanning microscope (LSM510; Carl Zeiss, Jena, Germany).
5
Immunofluorescence Analysis of Epithelial Cell Markers
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
A253 cells and HSDE cells grown in 35 mm glass-coated wells (Iwaki, Chiba, Japan) were fixed with cold acetone and ethanol (1:1) at –20 °C for 10 min. After rinsing in PBS, the cells were incubated with anti-p63, anti-LSR, anti-OCLN, anti-CGN, anti-ZO-3, anti-TRIC, anti-CLDN-4, anti-acetylated-tubulin (1:100), and anti-cytokeratin 5 (1:200) antibodies and Alexa 594-phalloidin (1:200) overnight at 4 °C. Alexa Fluor 488 (green)-conjugated anti-rabbit IgG and Alexa Fluor 594 (red)-conjugated anti-mouse IgG (Invitrogen) were used as secondary antibodies. The specimens were examined and photographed with an Olympus IX 71 inverted microscope (Olympus Co.; Tokyo, Japan) and a confocal laser scanning microscope (LSM510; Carl Zeiss, Jena, Germany).
6
Immunofluorescent Localization of Tight Junction Proteins in hTERT-transfected HNECs
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
hTERT-transfected HNECs grown in 35mm glass-coated wells (Iwaki, Chiba, Japan), were fixed with cold acetone and ethanol (1:1) at −20 °C for 10 min. After rinsing in PBS, the cells were incubated with anti-LSR, anti-TRIC, anti-CLDN-1, anti-CLDN-4, anti-OCLN, and anti-p63 antibodies (1:100) overnight at 4 °C. Alexa Fluor 488 (green)-conjugated anti-rabbit IgG and Alexa Fluor 592 (red)-conjugated anti-mouse IgG (Invitrogen) were used as secondary antibodies. The specimens were examined and photographed with an Olympus IX 71 inverted microscope (Olympus Co.; Tokyo, Japan) and a confocal laser scanning microscope (LSM510; Carl Zeiss, Jena, Germany).
7
Immunofluorescence Analysis of Epithelial Markers
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The cultured cells in 35-mm glass-coated wells (Iwaki, Chiba, Japan) were fixed with cold acetone and ethanol (1:1) at −20°C for 10 min. After rinsing in PBS, the cells were incubated with anti-cytokeratin7 (1:200), anti-p63 (1:100), anti ΔNp63 (1:100), anti-GATA-3 (1:100), anti-JAM-A (1:100) and anti-β-catenin (1:100) antibodies at room temperature for 1 h. Alexa Fluor 488 (green)-conjugated anti-rabbit IgG and Alexa Fluor 594 (red)-conjugated anti-mouse IgG (Invitrogen) were used as secondary antibodies. The specimens were examined using an epifluorescence microscope (Olympus, Tokyo, Japan) and a confocal laser scanning microscope (LSM5; Carl Zeiss, Jena, Germany).
8
Characterization of hTERT-Transfected Human Nasal Epithelial Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
hTERT-transfected HNECs grown in 35mm glass-coated wells (Iwaki, Chiba, Japan), were fixed with cold acetone and ethanol (1:1) at –20 °C for 10 min. After rinsing in PBS, the cells were incubated with anti-CK5, anti-CK7, anti-p63, ΔNp63, anti-anti-RSV-G protein (Masaki et al., 2011), and anti-occludin, anti-CLDN-4, anti-LSR, anti-tricellulin, anti-Ac-tubulin and anti-γ-tubulin antibodies (1:100) overnight at 4 °C. Alexa Fluor 488 (green)-conjugated anti-rabbit IgG and Alexa Fluor 592 (red)-conjugated anti-mouse IgG (Invitrogen) were used as secondary antibodies. The specimens were examined and photographed with an Olympus IX 71 inverted microscope (Olympus Co.; Tokyo, Japan) and a confocal laser scanning microscope (LSM510; Carl Zeiss, Jena, Germany).
9
Immunofluorescence Analysis of A549 and HLE Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
A549 cells and HLE cells cultured in 35 mm glass-coated wells (Iwaki, Chiba, Japan) were fixed with a mixture of cold acetone and ethanol (1:1) at –20 °C for 10 min. Following a rinse in PBS, the cells were incubated overnight at 4 °C with anti-CGN and anti-CLDN-2 antibodies (1:100). Subsequently, the cells underwent three washes in PBS. Alexa Fluor 488 (green)-conjugated anti-rabbit IgG and Alexa Fluor 592 (red)-conjugated anti-mouse IgG (Invitrogen) were used as secondary antibodies. The specimens were observed and captured using an Olympus IX 71 inverted microscope (Olympus Co.; Tokyo, Japan) and a confocal laser scanning microscope (LSM510; Carl Zeiss, Jena, Germany).
10
Immunofluorescence Analysis of Tight Junctions
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The hTERT-transfected HNECs grown in 35-mm glass-coated wells (Iwaki, Chiba, Japan), were fixed with cold acetone and ethanol (1:1) at –20°C for 10 min. After rinsing in PBS, the cells were incubated with anti-occludin and anti-claudin-1 antibodies (Table 2 ) at room temperature for 1 h. Alexa Fluor 488 (green)-conjugated anti-rabbit IgG and Alexa Fluor 592 (red)-conjugated anti-mouse IgG (Invitrogen) were used as secondary antibodies. The specimens were examined using a confocal laser scanning microscope (LSM510; Carl Zeiss, Jena, Germany).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!