Immulite 2000 xpi platform

Manufactured by Siemens

Sourced in United States, Germany

The Immulite 2000 XPi platform is an automated immunoassay analyzer developed by Siemens. The platform is designed for in vitro diagnostic testing, providing automated sample processing and analysis capabilities. The Immulite 2000 XPi is capable of performing a variety of immunoassay tests, including those for hormones, therapeutic drugs, and infectious diseases.

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Lab products found in correlation

Cobas 8000 platform, by Roche (2 mentions) Cobas 6800, by Roche (1 mentions) Immulite 2000, by Siemens (1 mentions) Unicell dxi 800, by Beckman Coulter (1 mentions) Cobas 8000 modular analyzer, by Roche (1 mentions) Chs msms steroids kit, by PerkinElmer (1 mentions) Xevo tq s system, by Waters Corporation (1 mentions)

Spelling variants of this product

Immulite 2000 (453 citations) Immulite 2000 XPi (143 citations) Immulite XPi (13 citations) IMMULITE 2000 platform (2 citations)

10 protocols using immulite 2000 xpi platform

Comprehensive Hormonal Profile Assessment

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Measurements of cortisol and other hormones including free T4, free T3, TSH, LH, FSH, prolactin, testosterone, and oestradiol were performed by immunoassays on Roche Diagnostics COBAS e601 platform, while GH and ACTH were measured by immunoassays on Siemens IMMULITE 2000 XPi platform. Copeptin was measured with a new sandwich immunoassay, as described before [2 (link), 12 (link)]. This assay has a lower detection limit of 0.4 pmol/l; functional assay sensitivity at <20 % interassay CV, <1 pmol/l [2 (link)]. All samples were assayed as a batch analyzed in one run by courtesy of ThermoFisher Scientific, Hennigdorf, Germany.
The study was approved by the Ethics Committee of the Polish Mothers’ Memorial Research Institute, Lodz, Poland.

Lewandowski K.C., Lewiński A., Skowrońska-Jóźwiak E., Stasiak M., Horzelski W, & Brabant G. (2015). Copeptin under glucagon stimulation. Endocrine, 52, 344-351.

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Serum Cortisol Measurement Protocol

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CT was measured on IMMULITE^® 2000 XPi platform (Siemens Healthcare Diagnostics) until February 2019 and on Cobas 8000 platform (Roche Diagnostics) later, according to the manufacturer instructions.

Trimboli P., Camponovo C, & Ruinelli L. (2022). The dilemma of routine testing for calcitonin thyroid nodule’s patients to detect or exclude medullary carcinoma: one single negative test should be valuable as rule-out strategy to avoid further calcitonin measurements over time. Endocrine, 77(1), 80-85.

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Measurement of Thyroid Biomarkers

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The measurement of Ctn was performed on the Immulite 2000 XPi platform (Siemens Healthcare Diagnostics, Malvern, PA, USA) until February 2019 and on the Cobas 8000 platform (Roche Diagnostics, Basel, Switzerland) since March 2019. Thyroid hormones and T-Ab were measured on the Immulite 2000 platform (Siemens Healthcare Diagnostics) until 2018 and Cobas 6800 (Roche Diagnostics) later.

Trimboli P., Peloni G., Confalonieri D., Gamarra E., Piticchio T., Frasca F., Makovac P., Piccardo A, & Ruinelli L. (2024). Determinants of circulating calcitonin value: analysis of thyroid features, demographic data, anthropometric characteristics, comorbidities, medications, and smoking habits in a population with histological full exclusion of medullary thyroid carcinoma. Frontiers in Oncology, 14, 1278816.

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Comprehensive Metabolic Profiling Protocol

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Physical characteristics including weight, height, waist circumference, and blood pressure were measured by trained medical personnel. Blood pressure was measured using an appropriately sized cuff with the subject in a sitting position after at least 10 min of rest. Waist circumference was measured at the midline between the lowest rib and the iliac crest.
Blood samples were taken after an overnight fast of at least 12 h. White blood cell (WBC) counts were analyzed using a Sysmex-XE2100 automated blood cell analyzer (Sysmex, Kobe, Japan). Fasting plasma glucose (FPG), total cholesterol, triglyceride, high-density lipoprotein (HDL) cholesterol, and low-density lipoprotein cholesterol levels were measured using a Hitachi 7600 automated analyzer (Hitachi Co., Tokyo, Japan). Glycated hemoglobin (HbA1c) was measured using a Tosoh HLC-723 HbG7 analyzer (Tosoh Bioscience Ltd., Redditch, UK). Helicobacter pylori-specific immunoglobulin G concentration was measured using the Immulite 2000 XPi platform (Siemens Healthcare Diagnostics, Erlangen, Germany).

Baeg M.K., Ko S.H., Ko S.Y., Jung H.S, & Choi M.G. (2018). Obesity increases the risk of erosive esophagitis but metabolic unhealthiness alone does not: a large-scale cross-sectional study. BMC Gastroenterology, 18, 82.

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Thyroid Hormone Measurement Protocol

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Thyroid‐stimulating hormone (TSH; with additional fT4 measurement if abnormal) was measured on the UniCell® DxI 800 fully automated random access analyser (Beckman Coulter, Nyon, Switzerland) according to the manufacturers’ specifications, as previously described (reference range: 0.40‐4.00 mUI/L). PCT was measured by fully automated homogenous time‐resolved amplified cryptate emission (TRACE) immunometric fluorescent assay on the Kryptor® system (Brahms Thermo Fischer, Hennigsdorf, Germany). The limits of detection and quantification are 0.02 and 0.075 μg/L, respectively, with an upper reference limit of 0.1 μg/L. CT was measured on the IMMULITE®2000 XPi platform (Siemens Healthcare Diagnostics, Erlangen, Germany) in strict adherence to the manufacturer's instructions. The CT assay is standardized against the 2nd International WHO calibrator 89/620. The limits of detection and quantification are 2.00 and 5.00 ng/L, respectively. Upper reference limits, as previously settled in our centre, are 7.7 ng/L for males and 5.5 ng/L for females, respectively.31

Giovanella L., Imperiali M., Piccardo A., Taborelli M., Verburg F.A., Daurizio F, & Trimboli P. (2018). Procalcitonin measurement to screen medullary thyroid carcinoma: A prospective evaluation in a series of 2705 patients with thyroid nodules. European Journal of Clinical Investigation, 48(6), e12934.

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Hormonal Assessment Protocol for Research

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Sex hormone-binding globulin (SHBG) was measured using the Immulite 2000XPi platform (Siemens, Los Angeles, CA, USA), while thyroid-stimulating hormone (TSH) was measured on the Vitros Eci (Ortho Diagnostics, Raritan, NJ, USA). Oestradiol was measured using a COBAS 8000 Modular Analyzer (Roche Diagnostics, Rotkreuz, Switzerland). The corresponding interassay CV was <7%.
Androstenedione, testosterone and DHEAS were measured on a Waters XEVO TQ-S system (Waters, Milford, MA, USA) using the CHSMSMS Steroids Kit (Perkin Elmer, Turku, Finland). The interassay CVs of androstenedione, testosterone and DHEAS were <6.5%, <5% and <5.9%, respectively. Although assessment of blood measurements, including sex steroids, was performed on fasting samples, some of the participants came to the research centre in a non-fasting state.

Brahimaj A., Muka T., Kavousi M., Laven J.S., Dehghan A, & Franco O.H. (2016). Serum dehydroepiandrosterone levels are associated with lower risk of type 2 diabetes: the Rotterdam Study. Diabetologia, 60(1), 98-106.

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Steroid Hormone Profiling in Cell Culture

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At each experimental time point during culture (3, 6, 12, 24, 48, and 72 h), aliquots (0.5 mL) of culture medium were collected and preserved at −20°C for subsequent steroid assays. A fresh 0.5 mL aliquot of media, containing NGF treatment or control, was added to each well at each time point except 72 h when the experiment was completed. Progesterone, testosterone, and estradiol-17β concentrations at each time point in the culture media were assessed using immunoassays (Immulite 2000 XPi platform; Siemens Medical Solutions, Malvern, PA, USA, Inc.). Total hormone production for each well was calculated by multiplying the measured concentration by the volume of media (0.5 mL) and then dividing by follicle wall cells weight (mg). Intra-assay coefficient of variations were 4.0% (testosterone), 2.4% (progesterone), and 3.1% (estradiol-17β). Inter-assay coefficient of variations were 12% (testosterone), 19% (progesterone), and 15% (estradiol-17β). The progesterone assay had a detection range of 0.2–40 ng/mL and a sensitivity of 0.1 ng/mL. The testosterone assay had a detection range of 20–1,600 ng/mL and a sensitivity of 15 ng/dL. The estradiol-17β assay had a detection range of 20–2,000 pg/mL and a sensitivity of 15 pg/mL.

Stewart J.L., Gao L., Flaws J.A., Mercadante V.R., Dias N.W., Canisso I.F, & Lima F.S. (2022). Effects of Nerve Growth Factor-β From Bull Seminal Plasma on Steroidogenesis and Angiogenic Markers of the Bovine Pre-ovulatory Follicle Wall Cell Culture. Frontiers in Veterinary Science, 8, 786480.

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Thyroid Hormone Measurement Protocol

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TSH and calcitonin were measured using an IMMULITE® 2000 XPi platform (Siemens Healthcare Diagnostics, Erlangen, Germany). The normal limits were 0.40 – 4.00 mUI/L for TSH, and <8.4 ng/L (in men) and <5 ng/L (in women) for calcitonin.

Giovanella L., Campenni A., Treglia G., Verburg F.A., Trimboli P., Ceriani L, & Bongiovanni M. (2015). Molecular imaging with 99mTc-MIBI and molecular testing for mutations in differentiating benign from malignant follicular neoplasm: a prospective comparison. European Journal of Nuclear Medicine and Molecular Imaging, 43, 1018-1026.

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Ovarian Dynamics Assessment in Cows

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Transrectal ultrasound examination of ovarian structures was carried out on a subset of cows (NGF: n = 32; control: n = 36) using an Easi-Scan coupled with a 7.5-MHz linear probe (Easi-Scan, BCF Technologies). Ovaries were evaluated at d 0, 7, 14, and 19 post-timed AI. The presence of a CL ≥ 10 mm and the presence of a follicle ≥8 mm were recorded. Sizes of the CL, as well as follicles, were estimated using gridlines on the ultrasound image. Corpus luteum volume was calculated using the formula volume = 4/3 × 3.14 × 0.5D 3 , where D = the estimated CL diameter. If a fluid-filled cavity was detected within the CL, the cavity volume was determined similarly and subtracted from the total CL volume.
Whole blood samples were collected from the same subset of cows on which ultrasonography was performed on d 0, 7, 14, and 19 post-timed AI. Blood samples were collected into EDTA tubes and placed on ice within 30 min of collection. Within 4 h, blood samples were centrifuged at 2,000 × g for 20 min at 4°C. Plasma was separated and transferred into polypropylene vials and stored at -20°C until analysis. Progesterone concentrations were analyzed using a chemiluminescence assay (Immulite 2000 XPi platform; Siemens Medical Solutions USA Inc.). The plasma progesterone interassay coefficient of variation was 17.2%, whereas the intraassay coefficient of variation was 2.8%.

Hubner A.M., Canisso I.F., Peixoto P.M., Coelho WM J.r., Cunha L.L., Ribeiro L., Crump S, & Lima F.S. (2022). Effect of nerve growth factor-β administered at insemination for lactating Holstein dairy cows bred after timed-artificial insemination protocol. Journal of dairy science, 105(7).

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Serum Calcitonin Measurement Method

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In all patients serum CT was measured by an immunochemiluminometric assay on fully automated IMMULITE ® 2000 XPi platform (Siemens Healthcare Diagnostics, Erlangen, Germany). The CT assay is standardized against the 2nd International WHO calibrator 89/620 and was performed in strict adherence to the manufacturer instructions as stated in the package insert. The limit of detection and the limit of quantification of CT assay were 2.00 and 5.00 pg/mL; upper reference limit 5.4 pg/mL and 8.2 pg/mL for females and males, respectively.

Trimboli P., Lauretta R., Barnabei A., Valabrega S., Romanelli F., Giovanella L, & Appetecchia M. (2018). Procalcitonin as a postoperative marker in the follow-up of patients affected by medullary thyroid carcinoma. The International journal of biological markers, 33(2).

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