Pd169136

Cadmium chloride, poly-d-lysine (PDL), 4′,6-diamidino-2-phenylindole (DAPI), and protease inhibitor cocktail were purchased from Sigma (St Louis, MO, USA). Resveratrol, SP600125, U0126, and PD169136 (Sigma) were dissolved in dimethylsulfoxide to prepare 100 mM Resveratrol, 20 mM SP600125, 5 mM U0126, and 20 mM PD169136 as stock solutions, and stored at −20°C. Dulbecco’s modified Eagle’s medium, 0.05% Trypsin-EDTA, NEUROBASAL™ Media, and B27 Supplement were purchased from Invitrogen (Grand Island, NY, USA). Horse serum and fetal bovine serum were supplied by Hyclone (Logan, UT, USA). Enhanced chemiluminescence reagent was from Millipore (Billerica, MA, USA). The following antibodies were used: PP2ACα (BD Biosciences, San Jose, CA, USA), PP2A-A subunit, PP2A-B subunit (Millipore), JNK1, phospho-JNK (Thr183/Tyr185), c-Jun, phospho-c-Jun (Ser63), phospho-Erk1/2 (Thr202/Tyr204), p38, phospho-p38 (Thr180/Tyr182), cleaved-caspase-3 (Cell Signaling Technology, Beverly, MA, USA), Erk2, demethylated-PP2A, PP5, MKP-1 (Santa Cruz Biotechnology, Santa Cruz, CA, USA), phospho-PP2A (Epitomics, Burlingame, CA, USA), FLAG, hemagglutinin (HA), β-tubulin (Sigma), goat anti-rabbit IgG-horseradish peroxidase (HRP), goat anti-mouse IgG-HRP, and rabbit anti-goat IgG-HRP (Pierce, Rockford, IL, USA). Other chemicals were purchased from local commercial sources and were of analytical grade.

Cadmium chloride, poly-D-lysine (PDL), monodansylcadaverine (MDC), 4’,6-diamidino-2-phenylindole (DAPI), 3-methyladenine (3-MA), SP600125, U0126, PD169136, ethylene glycol tetra-acetic acid (EGTA), and protease inhibitor cocktail were purchased from Sigma (St. Louis, MO, USA). 1,2-bis (o-aminophenoxy) ethane-N,N,N’,N’-tetraacetic acid tetra (acetoxymethyl) ester (BAPTA/AM) and 2-aminoethoxydiphenyl borane (2-APB) were purchased from Calbiochem (San Diego, CA, USA). z-Val-Ala-Asp-CH₂F (zVAD-fmk) were acquired from ALEXIS Biochemicals Corporation (San Diego, CA). CellTiter 96®AQ_ueous One Solution Cell Proliferation Assay kit was from Promega (Madison, WI, USA). Enhanced chemiluminescence solution was from Sciben Biotech Company (Nanjing, China). Dulbecco’s modified Eagle medium (DMEM), 0.05% Trypsin–EDTA, NEUROBASAL^™ Media, and B27 Supplement were purchased from Invitrogen (Grand Island, NY, USA). Horse serum and fetal bovine serum (FBS) were supplied by Hyclone (Logan, UT, USA). Other chemicals were purchased from local commercial sources and were of analytical grade.