Human il 1 beta elisa kit

The level of IL-1β was determined from the medium of the cell cultures by Human IL-1 beta ELISA Kit (Abcam, ab214025) according to the manufacturer’s recommendations.

PTCs were transfected with si-ACE2 or pcDNA-ACE2, treated with oxLDL and further maintained at 37° for 24 h. The expression levels of the inflammatory cytokines TNF-α, IL-6, and IL-1β were quantified using human TNF alpha ELISA kit (Abcam, UK, ab181421), human IL-6 ELISA kit (Abcam, UK, ab178013) and human IL-1 beta ELISA kit (Abcam, UK, ab197742) respectively by following the manufacturer’s protocol. Optical density (OD) of both samples and standards was measured at 450 nm on the Tecan reader.

PTCs were transfected with si-ACE2 or pcDNA-ACE2, treated with oxLDL and further maintained at 37° for 24 h. The expression levels of the inflammatory cytokines TNF-α, IL-6, and IL-1β were quantified using human TNF alpha ELISA kit (Abcam, UK, ab181421), human IL-6 ELISA kit (Abcam, UK, ab178013) and human IL-1 beta ELISA kit (Abcam, UK, ab197742) respectively by following the manufacturer’s protocol. Optical density (OD) of both samples and standards was measured at 450 nm on the Tecan reader.

PTCs were transfected with si-ACE2 or pcDNA-ACE2, treated with oxLDL and further maintained at 37° for 24 h. The expression levels of the inflammatory cytokines TNF-α, IL-6, and IL-1β were quantified using human TNF alpha ELISA kit (Abcam, UK, ab181421), human IL-6 ELISA kit (Abcam, UK, ab178013) and human IL-1 beta ELISA kit (Abcam, UK, ab197742) respectively by following the manufacturer’s protocol. Optical density (OD) of both samples and standards was measured at 450 nm on the Tecan reader.

Determination of the content of pro-inflammatory cytokines TNFα, IL1β, and endogenous β-amyloid peptide Aβ40 in the lysate of human peripheral blood mononuclear cells was performed after 1 and 24 h of incubation with rAβ42 by immunoenzymatic method with spectrophotometric detection on a FL600 microplate multimodal reader (BioTek) using test kits “Human TNF alpha ELISA Kit” (Abcam, cat. no. ab181421), “Human IL-1 beta ELISA Kit” (Abcam, cat. no. ab214025) and “Amyloid beta 40 Human ELISA Kit” (Thermo Scientific, cat. No. #KHB3481) respectively. NP-40 buffer (Thermo Scientific, cat. no. J60766.AP) was used for cell lysis. Determination of total protein in cell lysate was carried out by the direct UV spectrophotometric method on a scanning spectrophotometer Ultrospec 3,100 pro (Biochrom). The content of cytokines and endogenous Aβ40 was calculated in ng/g of total protein.