BM-MSCs and normal fibroblast cells (WI38) were purchased from Cambrex Bioscience Walkersville (East Rutherford, NJ, USA) and ATCC (Manassas, VA, USA), respectively. Cells were cultured in alpha-MEM (Invitrogen, Carlsbad, CA, USA) containing 10% fetal bovine serum (FBS), 1% penicillin/streptomycin (P/S, 100 mg/mL; Gibco-BRL, NY, USA), and 2 mM l -glutamine (Gibco-BRL). PD-MSCs were harvested from the inner side of the chorioamniotic membrane of the placenta as described previously (16 (link)). PD-MSCs and BM-MSCs at passages 6 to 8 were used for assays. The cells were treated with 50 μg/mL of mitomycin C (MMC; Sigma-Aldrich) for 50 min to stop cell division and then used as feeder cells for co-culture with naïve or stimulated T cells isolated from peripheral blood (PB).
Bm mscs
Manufactured by Cambrex
Sourced in United States
The BM-MSCs is a laboratory equipment designed for the isolation, expansion, and characterization of bone marrow-derived mesenchymal stem cells (BM-MSCs). It provides a controlled environment for the cultivation and analysis of these important cell types.
Automatically generated - may contain errors
Lab products found in correlation
Alpha mem, by Thermo Fisher Scientific (2 mentions)
Mitomycin c mmc, by Merck Group (1 mentions)
Penicillin streptomycin p s, by Thermo Fisher Scientific (1 mentions)
L glutamine, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Gentamicin, by Thermo Fisher Scientific (1 mentions)
3 protocols using bm mscs
1
Mitomycin C-treated MSCs for T-cell co-culture
2
Characterization of Adult and Induced Pluripotent Stem Cell-Derived Mesenchymal Stem Cells
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Characterized BM-MSCs from healthy adults were commercially acquired from Cambrex BioScience (Cat. No. PT-2501). Human iPSC-MSCs derived from iPSC lines have been previously described22 (link). At least two iPSC-MSC lines (IMR90-iPSC-MSCs and Lee NL-iPSC-MSCs; passage 9 ~ 10) and two BM-MSC lines (passage 4 ~ 5) were used in this study. MSCs were cultured with Dulbecco’s Modified Eagle’s medium (DMEM) plus 10% fetal calf serum (GIBCO), basic fibroblast growth factor (bFGF, 5 ng/mL), and epidermal growth factor (EGF, 10 ng/mL). Cells were diluted at a ratio of 1:3 when they reached 70–80% confluence.
3
Isolation and Senescence of Mesenchymal Stem Cells
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Wharton’s jelly-derived mesenchymal stem cells (WJ-MSCs), adipose tissue-derived mesenchymal stem cells (AD-MSCs), placenta-derived mesenchymal stem cells (PL-MSCs), and umbilical cord blood-derived mesenchymal stem cells (UCB-MSCs) were isolated from umbilical cord, adipose tissue, placenta, and umbilical cord blood, respectively, as previously described (12 (link)15 (link, link, link)). Human bone marrow-derived mesenchymal stem cells (BM-MSCs) were purchased from Cambrex (#PT-2501; Lonza). Replicative senescence was induced by sequential culture in MEM-alpha (Minimum Essential Medium; Gibco) with 10% FBS (Fetal Bovine Serum; Gibco) and 50 μg/ml Gentamicin (Gibco) at 37℃ and 5% CO2.
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