The sample, placed in a volumetric flask, was dissolved and extracted in isopropanol in an ultrasonic water bath for 30 min. An obtained aliquot of the extract was filtered in vials with PTFE filters (0.45 μm). The final determination was carried out in the liquid chromatograph 1290 infinity series (Agilent Technologies, Lake Forest, CA, USA) with the column Kinetex 1.7u PFP 100A (150 × 2.10 mm), with a fluorometric detector 1290 infinity series (Agilent Technologies, Lake Forest, CA, USA). The mobile phases (Table 3 ) were 0.04% phosphoric acid in water + 5% acetonitrile for A and 100% acetonitrile for B. The analysis was performed according to the conditions described in the Commission Directive 2000/45/EC [37 ].
1290 infinity series
Manufactured by Agilent Technologies
Sourced in United States
The 1290 Infinity Series is a line of high-performance liquid chromatography (HPLC) instruments manufactured by Agilent Technologies. The series is designed to provide reliable and efficient separation, detection, and analysis of a wide range of chemical compounds. The core function of the 1290 Infinity Series is to enable accurate and precise separation and quantification of analytes in complex samples.
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Lab products found in correlation
Zorbax eclipse plus c18 column, by Agilent Technologies (1 mentions)
Qtrap 6500 mass spectrometer, by AB Sciex (1 mentions)
Lc 20ad, by Shimadzu (1 mentions)
Eclipse plus c18 column, by Agilent Technologies (1 mentions)
Luna 5 μm c18 2, by Phenomenex (1 mentions)
6540 uhd accurate mass q tof spectrometer, by Agilent Technologies (1 mentions)
Eclipse xdb c18 column, by Agilent Technologies (1 mentions)
Acclaim rslc explosives e2 column, by Thermo Fisher Scientific (1 mentions)
6230 tof mass spectrometer, by Agilent Technologies (1 mentions)
6460 triple quadrupole mass spectrometer, by Agilent Technologies (1 mentions)
Zorbax rrhd eclipse plus c18 column, by Agilent Technologies (1 mentions)
Oasis hlb cartridge, by Waters Corporation (1 mentions)
Masshunter workstation, by Agilent Technologies (1 mentions)
6230 time of flight, by Agilent Technologies (1 mentions)
G6545a quadrupole time of flight, by Agilent Technologies (1 mentions)
Hilic column, by Waters Corporation (1 mentions)
Aluminum silica gel 60 f254 tlc plates, by Merck Group (1 mentions)
Maxis impact time of flight spectrophotometer, by Bruker (1 mentions)
Acquity uplc hss t3 column, by Waters Corporation (1 mentions)
Rf 10axl, by Shimadzu (1 mentions)
14 protocols using 1290 infinity series
1
HPLC Quantification of Compounds
2
Quantification of Biliverdin and Biliverdin-d4
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Biliverdin and biliverdin-d4 were analyzed on an AB-SCIEX model Q-Trap 6500+ mass spectrometer (AB-SCIEX, Framingham, MA, USA) interfaced with an Agilent 1290 Infinity Series ultra-performance liquid chromatography system. Chromatographic separation was carried out on an Agilent ZORBAX Eclipse Plus C18 column (1.8 μm, 2.1 × 50 mm) using gradient elution at a flow rate of 0.2 mL/min for 10 min. The mobile phases were composed of 0.1% (v/v) formic acid in water (A) and 0.1% (v/v) formic acid in methanol (B). The linear gradient was as follows: 30% B, 0−1 min; 30−99% B 1–1.1 min; and 99% B 1.1−6.5 min. Biliverdin and biliverdin-d4 (retention time 2.7 min) were detected using electrospray ionization in the positive ion mode by multiple reaction monitoring scanning. The mass spectrometer parameters were optimized and set as follows: curtain gas, gas 1, and gas 2 were 30, 25, and 25 psi, respectively; ion source temperature was at 500 °C; and ion spray voltage, declustering potential, entrance potential, and collision cell exit potential were 5500, 90, 10, and 12 V, respectively. The transitions (and corresponding collision energy) were m/z 583.1/297.1 (45) for biliverdin and m/z 587.3/299.2 (42) for biliverdin-d4. All data were analyzed using the AB SCIEX Analyst 1.6.3 software (Applied Biosystems, Framingham, MA, USA).
3
Analytical Quantification of CBZ, PRO, and PFOS
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Concentrations of CBZ and PRO were determined using HPLC (Shimadzu LC-20AD) equipped with an Eclipse Plus C18 column (1.8 μm, 4.6 × 50 mm, Agilent) and a UV/Vis detector (SPD-20AV). A sample volume of 100 μL was injected using an isocratic mobile phase of solvent comprising 35% acetonitrile and 65% water with 0.1% (v/v) phosphoric acid at a flow rate of 1.3 mL min−1. PFOS concentration was determined using HPLC (Agilent 1290 Infinity Series) coupled with a mass spectrometer (Agilent 6550A iFunnel Q-TOF MS). A sample volume of 1 μL was injected into an Eclipse Plus C18 column using a mobile phase gradient of solvent comprised of 40% methanol and 60% water with 20 mM ammonium acetate at a flow rate of 0.5 mL min−1. Methanol was increased from 40% to 98% in 5 min, held for 1 min, dropped back to 40%, and stabilized for 1 min. The Q-TOF MS was operated using an electrospray ionization (ESI) interface in negative mode.
4
PFAS Analysis in Serum and Placenta
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The targeted analysis of PFAS in the serum and placental tissue samples was performed by high-performance liquid chromatography coupled with tandem-mass spectrometry (HPLC-MS/MS). This analytical system was composed of an Agilent Technologies 1290 Infinity Series (Agilent Technologies, Santa Clara, CA, USA) HPLC and a SCIEX 4000 QTRAP mass spectrometer (AB Sciex Technologies, Framingham, MA, USA) in electrospray ionization (ESI) negative mode. The analytical column was a Luna 5 μm C18(2), 100 × 2 mm (Phenomenex, CA, USA). The eluents were methanol (mobile phase B) and LC-MS grade water, containing 10 mM ammonium acetate (mobile phase A). Details of the LC program are provided in the ESM .
5
UHPLC-QTOF MS Characterization Protocol
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High-resolution MS spectra were recorded with Agilent Technologies 6540 UHD Accurate-Mass Q-TOF spectrometer in positive electrospray ionization mode. The chromatographic separation was performed using the chromatographic system consisting of a pump, degasser, autosampler and column oven from the Agilent 1290 Infinity series. The separation of analytes was achieved using a Agilent Eclipse XDB-C18 column (150 mm × 4.6 mm, 5 μm, pore size 80 Å). The mobile phase contained 39% acetonitrile/61% ammonium acetate buffer (5.5 mmol, pH = 4.5), v/v; at a flow rate of 0.6 mL/min.
6
Quantitative UHPLC-DAD Analysis of Explosive Compounds
7
Quadrupole-TOF Mass Spectrometry Protocol
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In general, methods described in our previous work, which used an Agilent G6545A quadrupole-time of flight (TOF) or an Agilent 6230 TOF mass spectrometer equipped with a dual AJS ESI source and an Agilent 1290 Infinity series diode array detector, autosampler, and binary pump, were followed here7 (link). Solvent A = water with 0.1% formic acid. Solvent B = 95% acetonitrile, 5% water and 0.1% formic acid. An Acquity UPLE BEH Amide 1.7 µm, 2.1 × 100 mm hydrophobic interaction liquid chromatography column was used for all separations (Waters). The chromatographic method was typically 18% A 0–5 min at 0.4 mL/min or 15% A 0–7 min at 0.3 mL/min. 1.0 µL injections were made for each sample.
8
Isolation and Structural Elucidation of Kratom Alkaloids
9
Quantification of Analytes in Rat Biosamples
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Rat plasma or CSF (100 μL) was loaded on a Waters Oasis HLB cartridge preconditioned with methanol and water, which was washed with 1.0 mL water, sucked dry, and eluted with 1.0 mL of methanol with the flow rate of 1 mL/min. The eluate was evaporated to dry under a gentle stream of nitrogen at 40 °C and the residue was reconstituted using 100 μL methanol. After being centrifuged at 9,000 g, 5 μL of the supernatant was injected into the UPLC-MS/MS system for analysis.
The UPLC-MS/MS analyses were performed using an Agilent 1290 Infinity series connected to an Agilent 6460 Triple Quadrupole Mass Spectrometer with an ESI source. Chromatographic separation was achieved on a Agilent ZORBAX RRHD Eclipse Plus C18 column (100 mm × 2.1 mm I.D., 3 μm) at 35 °C, and the eluent was aqueous formic acid (100:0.1, V/V) (A)-acetonitrile (B) at a flow rate of 0.3 mL/min. The gradient elution was performed as follows: 16%–33% B at 0–8 min, and 33%–99% B at 8–19 min. The capillary voltage was 3500 V/–4000 V. MS detection of the samples was performed by multiple reaction monitoring in both ion modes by comparison with reference standards. Drying gas temperature was set at 350 °C with a velocity of 9 L/min and nebuliser pressure at 40 psi. A MassHunter Workstation (Version B.06.00, Agilent Technologies) was used to process the data.
The UPLC-MS/MS analyses were performed using an Agilent 1290 Infinity series connected to an Agilent 6460 Triple Quadrupole Mass Spectrometer with an ESI source. Chromatographic separation was achieved on a Agilent ZORBAX RRHD Eclipse Plus C18 column (100 mm × 2.1 mm I.D., 3 μm) at 35 °C, and the eluent was aqueous formic acid (100:0.1, V/V) (A)-acetonitrile (B) at a flow rate of 0.3 mL/min. The gradient elution was performed as follows: 16%–33% B at 0–8 min, and 33%–99% B at 8–19 min. The capillary voltage was 3500 V/–4000 V. MS detection of the samples was performed by multiple reaction monitoring in both ion modes by comparison with reference standards. Drying gas temperature was set at 350 °C with a velocity of 9 L/min and nebuliser pressure at 40 psi. A MassHunter Workstation (Version B.06.00, Agilent Technologies) was used to process the data.
10
HILIC-QTOF-MS Metabolite Profiling
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Liquid chromatography-mass spectrometry (LC-MS) analysis was performed on an Agilent G6545A quadrupole-time of flight (QTOF) or Agilent 6230 time of flight (TOF) mass spectrometer equipped with a dual AJS ESI source and an Agilent 1290 Infinity series diode array detector, autosampler, and binary pump. Solvent A = water with 0.1% formic acid. Solvent B = 95% acetonitrile, 5% water and 0.1% formic acid. An Acquity UPLE BEH Amide 1.7 µm, 2.1 × 100 mm hydrophobic interaction liquid chromatography (HILIC) column from Waters was used for all separations. The chromatographic method was typically 18% A 0–5 min at 0.4 mL/min or 15% A 0–7 min at 0.3 mL/min. In all, 0.3–10 µL injections were made for each sample. Targeted MS/MS was performed using the QTOF using an 18% A isocratic method, 0–5 min, to obtain fragmentation patterns. Methods were augmented to target each mass. Collision energies were set to 10, 20, and 30 eV and the resulting chromatograms were averages of the three collision energies.
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