For flow cytometry, the following antibodies (Abs) were used: R-phycoerythrin (PE)/Cy7-conjugated mouse anti-human CD47 (clone CC2C6) monoclonal Ab (MAb) and allophycocyanin (APC)-conjugated anti-CD11b MAb (clone ICRF44) as well as corresponding isotype controls from BioLegend, APC-conjugated mouse anti-human CD47 MAb (clone B6H12, eBioscience), and phycoerythrin (RD1)-conjugated anti-Gag (clone KC57, Beckman Coulter). For immunoprecipitation and Western blotting, the following Abs were used: polyclonal sheep anti-human CD47 (AF4670) and sheep IgG horseradish peroxidase (HRP)-conjugated Ab (HAF016) from R&D Systems; mouse anti-hemagglutinin (HA) MAb (16B12), anti-GAPDH (FF26A/F9), and anti-CRISPR Cas9 (7A9) from BioLegend; rabbit anti-HA MAb (C29F4) from Cell Signaling Technology; rabbit anti-GFP (SAB4301138) from Sigma-Aldrich; anti-β-actin (C4, sc-47778) from Santa Cruz Biotechnology; goat anti-rabbit IgG H+L (HRP, ab205718) and goat anti-mouse IgG H+L (HRP, ab 205719) from Abcam; and anti-Vpu rabbit polyclonal serum as described previously (76 (link)). For confocal microscopy analysis, the following Abs were used: purified anti-CD11b (clone ICRF44; BioLegend), anti-p17 as previously described (77 (link)), and Alexa Fluor 594-coupled donkey anti-mouse IgG H+L (Invitrogen, A-21203).
Apc conjugated anti cd11b mab clone icrf44
Manufactured by BioLegend
APC)-conjugated anti-CD11b MAb (clone ICRF44) is a monoclonal antibody conjugated with Allophycocyanin (APC) that binds to the CD11b antigen. CD11b is a cell surface glycoprotein that is expressed on the surface of various immune cells, including monocytes, macrophages, and granulocytes.
Automatically generated - may contain errors
Lab products found in correlation
Apc conjugated mouse anti human cd47 mab clone b6h12, by Thermo Fisher Scientific (2 mentions)
Rd1 conjugated anti gag clone kc57, by Beckman Coulter (2 mentions)
Anti crispr cas9 7a9, by BioLegend (2 mentions)
Anti ha c29f4 rabbit mab, by Cell Signaling Technology (2 mentions)
Rabbit anti gfp sab4301138, by Merck Group (2 mentions)
Purified anti cd11b clone icrf44, by BioLegend (2 mentions)
Alexa fluor 594 coupled donkey anti mouse igg h l, by Thermo Fisher Scientific (2 mentions)
Goat anti mouse igg h l hrp ab205719, by Abcam (1 mentions)
Ab205719, by Abcam (1 mentions)
Ab205718, by Abcam (1 mentions)
Goat anti mouse igg h l, by Abcam (1 mentions)
Goat anti rabbit igg h l, by Abcam (1 mentions)
2 protocols using apc conjugated anti cd11b mab clone icrf44
1
Multiparameter Flow Cytometry and Protein Detection Assay
2
Flow Cytometry and Immunoblotting Antibodies
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For flow cytometry, the following antibodies (Abs) were used: PE/Cy7-conjugated mouse antihuman CD47 (clone CC2C6) monoclonal Ab (mAb) and APC-conjugated anti-CD11b mAb (clone ICRF44) as well as corresponding isotype controls from BioLegend, APC-conjugated mouse anti-human CD47 mAb (clone B6H12; eBioscience), RD1-conjugated anti-Gag (clone KC57; Beckman Coulter). For immunoprecipitation and Western blot analysis, the following Abs were used: polyclonal sheep anti-human CD47 (AF4670) and sheep IgG HRPconjugated Ab (HAF016) from R&D system; mouse anti-HA mAb (16B12), anti-GAPDH (FF26A/F9) and anti-CRISPR Cas9 (7A9) from BioLegend; rabbit anti-HA mAb (C29F4) from Cell Signaling Technology; rabbit anti-GFP (SAB4301138) from Sigma-Aldrich; anti-β-actin (C4, sc-47778) from Santa Cruz Biotechnology; goat anti-rabbit IgG H+L (HRP, ab205718) and goat anti-mouse IgG H+L (HRP, ab 205719) from Abcam and anti-Vpu rabbit polyclonal serum as described previously (72) . For confocal microscopy analysis, the following Abs were used: purified anti-CD11b (clone ICRF44; BioLegend), anti-p17 as previously described (73) , Alexa Fluor 594-coupled donkey anti-mouse IgG H+L (Invitrogen, #A-21203).
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