1290 infinity uv detector

Manufactured by Agilent Technologies

The 1290 Infinity UV detector is a high-performance liquid chromatography (HPLC) detector from Agilent Technologies. It provides sensitive and reliable UV absorbance detection for HPLC applications. The detector offers a wide wavelength range, fast data acquisition, and advanced diagnostic capabilities.

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Lab products found in correlation

Xselect hss t3 column, by Waters Corporation (2 mentions) 6490 triple quad lc ms, by Agilent Technologies (2 mentions) 6490 triple quad mass detector, by Agilent Technologies (2 mentions)

Close spelling variants of this product

1290 Infinity (160 citations) UV detector (31 citations)

2 protocols using 1290 infinity uv detector

Measuring Global DNA Methylation and Hydroxymethylation

Cited 1 time

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Global cytosine methylation and hydroxymethylation levels were determined using established HPLC protocols (Ramsahoye, 2002 (link)) with some modifications. Briefly, genomic DNA was hydrolyzed with a combination of RNase A and RNase T1 followed by ethanol precipitation. DNA was digested to nucleosides as described previously (Hashimoto et al., 2014 (link)) and separated on a 6490 Triple Quad LC-MS with UV absorbance detector (1290 Infinity UV detector, 6490 Triple Quad Mass detector, Agilent, Santa Clara, CA) equipped with an XSelect™ HSS T3 column (2.1 × 100mm, 2.5 μm, Waters, Milford, MA).

Aluru N., Kuo E., Helfrich L.W., Karchner S.I., Linney E.A., Pais J.E, & Franks D.G. (2015). Developmental exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin alters DNA methyltransferase (dnmt) expression in zebrafish (Danio rerio). Toxicology and applied pharmacology, 284(2), 142-151.

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Quantifying Global Cytosine Levels

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Global cytosine levels were determined using established high performance liquid chromatography (HPLC) protocols (Ramsahoye, 2002 (link)) with some modifications. Briefly, genomic DNA was hydrolyzed with a combination of RNase A and RNase T1 followed by ethanol precipitation. DNA was digested to nucleosides as described previously (Hashimoto et al., 2015 (link)) and separated on a 6490 Triple Quad LC‐MS with UV absorbance detector (1290 Infinity UV detector, 6490 Triple Quad Mass detector, Agilent, Santa Clara, CA) equipped with an XSelect™ HSS T3 column (2.1 × 100 mm, 2.5 μm, Waters, Milford, MA).

Aluru N., Fields D.M., Shema S., Skiftesvik A.B, & Browman H.I. (2021). Gene expression and epigenetic responses of the marine Cladoceran, Evadne nordmanni, and the copepod, Acartia clausi, to elevated CO2. Ecology and Evolution, 11(23), 16776-16785.

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