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Biotinylated anti cd103 antibody

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Biotinylated anti-CD103 antibody is a laboratory reagent used for the detection and analysis of CD103 protein expression in biological samples. It is a monoclonal antibody conjugated with biotin, which enables its use in various immunoassay techniques such as flow cytometry, immunohistochemistry, and Western blotting.

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Lab products found in correlation

Confocal microscope, by Leica (2 mentions) Anti biotin microbead, by Miltenyi Biotec (2 mentions) Rhodamine labeled poly 1 c, by InvivoGen (2 mentions)

Close spelling variants of this product

CD103 (14 citations) Anti-CD103 (4 citations)

2 protocols using biotinylated anti cd103 antibody

1

Intranasal Delivery of Apoptotic Cells and OVA

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CFSE-labeled apoptotic cells and 1 μg soluble OVA-Alexa-647, or soluble OVA-FITC were co-delivered intranasally with 5 μg Rhodamine-labeled poly(I:C) (Invivogen). Two hours post delivery, lungs were digested and CD103+ DCs were isolated using biotinylated anti-CD103 antibody (ebioscience) and anti-biotin MicroBeads (Miltenyi Biotec). Cells were then cytospun, mounted with DAPI, and imaged using a Leica confocal microscope.
Desch A.N., Gibbings S.L., Clambey E.T., Janssen W.J., Slansky J.E., Kedl R.M., Henson P.M, & Jakubzick C. (2014). Dendritic cell subsets require cis-activation for cytotoxic CD8 T cell induction. Nature communications, 5, 4674.
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2

Intranasal Delivery of Apoptotic Cells and OVA

Check if the same lab product or an alternative is used in the 5 most similar protocols
CFSE-labeled apoptotic cells and 1 μg soluble OVA-Alexa-647, or soluble OVA-FITC were co-delivered intranasally with 5 μg Rhodamine-labeled poly(I:C) (Invivogen). Two hours post delivery, lungs were digested and CD103+ DCs were isolated using biotinylated anti-CD103 antibody (ebioscience) and anti-biotin MicroBeads (Miltenyi Biotec). Cells were then cytospun, mounted with DAPI, and imaged using a Leica confocal microscope.
Desch A.N., Gibbings S.L., Clambey E.T., Janssen W.J., Slansky J.E., Kedl R.M., Henson P.M, & Jakubzick C. (2014). Dendritic cell subsets require cis-activation for cytotoxic CD8 T cell induction. Nature communications, 5, 4674.
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