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Lmax microplate reader

Manufactured by Molecular Devices
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The LMax Microplate Reader is a multi-mode microplate reader designed for accurate and reliable absorbance measurements. It is capable of reading a wide range of microplate formats and offers a broad wavelength range for diverse applications.

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Lab products found in correlation

Gsh glo glutathione assay, by Promega (2 mentions) Celltiter glo luminescent cell viability assay, by Promega (2 mentions) Glutathione assay kit, by Nanjing Jiancheng (1 mentions)

Spelling variants of this product

Microplate reader (2507 citations) LMax II Microplate Reader (4 citations) LMax Luminometer Microplate Reader (2 citations)

3 protocols using lmax microplate reader

1

Glutathione Quantification in Macrophages

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GSH level in macrophages and artery lysates was analyzed by glutathione assay kit (Jiancheng, Nanjing, China) following the manufacturer's instruction. After the macrophages are treated, 100 μl of cold PBS was added and the macrophages were collected. 100 μl of precipitant was added into the macrophages. After centrifuged at 3500 rpm for 10 min, supernatant was extracted and mixed with 125 μl working solution for 5min. Luminescence was measured at 405 nm by LMax Microplate Reader (Molecular Devices). GSH concentration in each group was normalized to protein concentration.
Luo X., Wang Y., Zhu X., Chen Y., Xu B., Bai X., Weng X., Xu J., Tao Y., Yang D., Du J., Lv Y., Zhang S., Hu S., Li J, & Jia H. (2023). MCL attenuates atherosclerosis by suppressing macrophage ferroptosis via targeting KEAP1/NRF2 interaction. Redox Biology, 69, 102987.
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2

Erastin-Induced Glutathione Depletion in HT-1080 Cells

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HT-1080 cells (2,000 cells/well) were seeded in a white 96-well plate with clear bottom. The next day, cells were treated with IFNγ for 24 hours and followed with erastin treatment for 6 hours. GSH level was measured by a GSH-Glo Glutathione Assay (Promega) kit following the manufacturers’ instruction. Briefly, after treatment the culture medium was carefully removed, 100µl of 1X GSH-Glo™ Reagent was directly added to each well and followed with 30-minute incubation. Then, 100 μl of reconstituted Luciferin Detection Reagent was added and mixed. After 15 minutes, luminescence was measured using a LMax Microplate Reader (Molecular Devices). A standard curve for GSH concentration was generated along with samples and used for calculation. In the meantime, cell viability in another set of wells was measured using a CellTiter-GLO Luminescent Cell Viability Assay (Promega) kit. Briefly, a volume of CellTiter-Glo Reagent equal to the volume of cell culture medium present in each well was added and mixed to induce cell lysis. The plate was incubated for 10 minutes at room temperature. Luminescence was measured using a LMax Microplate Reader. Relative cell viability was calculated in comparison with control group (100% cell viability). GSH concentration in each group was normalized by the cell viability.
Wang W., Green M., Choi J.E., Gijón M., Kennedy P.D., Johnson J.K., Liao P., Lang X., Kryczek I., Sell A., Xia H., Zhou J., Li G., Li J., Li W., Wei S., Vatan L., Zhang H., Szeliga W., Gu W., Liu R., Lawrence T., Lamb C., Tanno Y., Cieslik M., Stone E., Georgiou G., Chan T.A., Chinnaiyan A, & Zou W. (2019). CD8+ T cells regulate tumor ferroptosis during cancer immunotherapy. Nature, 569(7755), 270-274.
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3

Erastin-Induced Glutathione Depletion in HT-1080 Cells

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HT-1080 cells (2,000 cells/well) were seeded in a white 96-well plate with clear bottom. The next day, cells were treated with IFNγ for 24 hours and followed with erastin treatment for 6 hours. GSH level was measured by a GSH-Glo Glutathione Assay (Promega) kit following the manufacturers’ instruction. Briefly, after treatment the culture medium was carefully removed, 100µl of 1X GSH-Glo™ Reagent was directly added to each well and followed with 30-minute incubation. Then, 100 μl of reconstituted Luciferin Detection Reagent was added and mixed. After 15 minutes, luminescence was measured using a LMax Microplate Reader (Molecular Devices). A standard curve for GSH concentration was generated along with samples and used for calculation. In the meantime, cell viability in another set of wells was measured using a CellTiter-GLO Luminescent Cell Viability Assay (Promega) kit. Briefly, a volume of CellTiter-Glo Reagent equal to the volume of cell culture medium present in each well was added and mixed to induce cell lysis. The plate was incubated for 10 minutes at room temperature. Luminescence was measured using a LMax Microplate Reader. Relative cell viability was calculated in comparison with control group (100% cell viability). GSH concentration in each group was normalized by the cell viability.
Wang W., Green M., Choi J.E., Gijón M., Kennedy P.D., Johnson J.K., Liao P., Lang X., Kryczek I., Sell A., Xia H., Zhou J., Li G., Li J., Li W., Wei S., Vatan L., Zhang H., Szeliga W., Gu W., Liu R., Lawrence T., Lamb C., Tanno Y., Cieslik M., Stone E., Georgiou G., Chan T.A., Chinnaiyan A, & Zou W. (2019). CD8+ T cells regulate tumor ferroptosis during cancer immunotherapy. Nature, 569(7755), 270-274.
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