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Anti cd38 pe cy7 hit2

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Anti-CD38–PE/Cy7 (HIT2) is a fluorophore-conjugated monoclonal antibody that specifically binds to the human CD38 antigen. CD38 is a transmembrane glycoprotein expressed on various cell types, including plasma cells, activated T and B lymphocytes, and natural killer cells.

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Lab products found in correlation

Cytofix cytoperm fixation permeabilization kit, by BD (2 mentions) Live dead fixable aqua dead cell stain, by Thermo Fisher Scientific (2 mentions) Rpa t8, by BioLegend (2 mentions) Hcd56, by BioLegend (2 mentions) Rpa t4, by BioLegend (2 mentions) Streptavidin apc cy7, by BioLegend (2 mentions) Cd11b icrf44, by BioLegend (2 mentions) Clone rpa 2, by BioLegend (2 mentions) Cd7 124 1d1, by Thermo Fisher Scientific (2 mentions) Cd10 sn5c, by Thermo Fisher Scientific (2 mentions) Cd20 2h7, by Thermo Fisher Scientific (2 mentions) Anti human cd34 apc 8g12, by BD (2 mentions) Anti cd90 fitc 5e10, by BD (2 mentions) Anti cd45ra pb mem 56, by Thermo Fisher Scientific (2 mentions) Anti cd123 pe 7g3, by BD (2 mentions) Anti human ki 67 bv605 antibody, by BioLegend (2 mentions) Cd14 hcd14, by BioLegend (2 mentions) Cd19 hib19, by BioLegend (2 mentions) Lsrii flow cytometer, by BD (2 mentions) Anti cd19 fitc, by BD (1 mentions)

Close spelling variants of this product

CD38-PE-Cy7 (30 citations) Anti-CD38-PE (13 citations) Anti-CD38 (12 citations) CD38-PE (9 citations)

3 protocols using anti cd38 pe cy7 hit2

1

Flow Cytometric Immunophenotyping of Hematopoietic Progenitor Cells

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Cells were thawed at 37 °C, washed with sterile PBS and incubated with biotinylated anti-human lineage antibodies directed against CD2 (clone RPA-2.10, BioLegend), CD3 (HIT3a, 300304, BioLegend), CD4 (RPA-T4, BioLegend), CD7 (124-1D1, eBioscience), CD8a (RPA-T8, BioLegend), CD10 (SN5c, eBioscience), CD11b (ICRF44, BioLegend), CD14 (HCD14, BioLegend), CD19 (HIB19, BioLegend), CD20 (2H7, eBioscience), CD56 (HCD56, BioLegend) and GPA (HIR2, BioLegend) and LIVE/DEAD Fixable Aqua Dead Cell Stain (L-34957, Life Technologies). For gating on hematopoietic progenitor cells, this was followed by secondary staining with anti-human CD34–APC (8G12, BD Biosciences), anti-CD38–PE/Cy7 (HIT2, BioLegend), anti-CD90–FITC (5E10, BD Biosciences), anti-CD45RA-PB (MEM-56, Thermo Fisher Scientific), anti-CD123–PE (7G3, BD Biosciences) and streptavidin-APC/Cy7 (BioLegend). Intracellular staining with anti-human Ki-67–BV605 antibody (BioLegend) was performed using the BD Cytofix/Cytoperm Fixation/Permeabilization kit. Samples were run on an LSR II flow cytometer (BD Biosciences), and recorded events were analyzed with FlowJo 10 software (BD). Individual fluorescence-minus-one controls were used to determine gating. HSCs were identified as lineage−CD34+CD38−CD45RAloCD90+ cells, CMPs as lineage−CD34+CD38intCD45 RA−CD123int cells and GMPs as lineage−CD34+CD38intCD45RA+CD123int cells.
Rohde D., Vandoorne K., Lee I.H., Grune J., Zhang S., McAlpine C.S., Schloss M.J., Nayar R., Courties G., Frodermann V., Wojtkiewicz G., Honold L., Chen Q., Schmidt S., Iwamoto Y., Sun Y., Cremer S., Hoyer F.F., Iborra-Egea O., Muñoz-Guijosa C., Ji F., Zhou B., Adams R.H., Wythe J.D., Hidalgo J., Watanabe H., Jung Y., van der Laan A.M., Piek J.J., Kfoury Y., Désogère P.A., Vinegoni C., Dutta P., Sadreyev R.I., Caravan P., Bayes-Genis A., Libby P., Scadden D.T., Lin C.P., Naxerova K., Swirski F.K, & Nahrendorf M. (2021). Bone marrow endothelial dysfunction promotes myeloid cell expansion in cardiovascular disease. Nature cardiovascular research, 1(1), 28-44.
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2

Flow Cytometric Immunophenotyping of Hematopoietic Progenitor Cells

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Cells were thawed at 37 °C, washed with sterile PBS and incubated with biotinylated anti-human lineage antibodies directed against CD2 (clone RPA-2.10, BioLegend), CD3 (HIT3a, 300304, BioLegend), CD4 (RPA-T4, BioLegend), CD7 (124-1D1, eBioscience), CD8a (RPA-T8, BioLegend), CD10 (SN5c, eBioscience), CD11b (ICRF44, BioLegend), CD14 (HCD14, BioLegend), CD19 (HIB19, BioLegend), CD20 (2H7, eBioscience), CD56 (HCD56, BioLegend) and GPA (HIR2, BioLegend) and LIVE/DEAD Fixable Aqua Dead Cell Stain (L-34957, Life Technologies). For gating on hematopoietic progenitor cells, this was followed by secondary staining with anti-human CD34–APC (8G12, BD Biosciences), anti-CD38–PE/Cy7 (HIT2, BioLegend), anti-CD90–FITC (5E10, BD Biosciences), anti-CD45RA-PB (MEM-56, Thermo Fisher Scientific), anti-CD123–PE (7G3, BD Biosciences) and streptavidin-APC/Cy7 (BioLegend). Intracellular staining with anti-human Ki-67–BV605 antibody (BioLegend) was performed using the BD Cytofix/Cytoperm Fixation/Permeabilization kit. Samples were run on an LSR II flow cytometer (BD Biosciences), and recorded events were analyzed with FlowJo 10 software (BD). Individual fluorescence-minus-one controls were used to determine gating. HSCs were identified as lineage−CD34+CD38−CD45RAloCD90+ cells, CMPs as lineage−CD34+CD38intCD45 RA−CD123int cells and GMPs as lineage−CD34+CD38intCD45RA+CD123int cells.
Rohde D., Vandoorne K., Lee I.H., Grune J., Zhang S., McAlpine C.S., Schloss M.J., Nayar R., Courties G., Frodermann V., Wojtkiewicz G., Honold L., Chen Q., Schmidt S., Iwamoto Y., Sun Y., Cremer S., Hoyer F.F., Iborra-Egea O., Muñoz-Guijosa C., Ji F., Zhou B., Adams R.H., Wythe J.D., Hidalgo J., Watanabe H., Jung Y., van der Laan A.M., Piek J.J., Kfoury Y., Désogère P.A., Vinegoni C., Dutta P., Sadreyev R.I., Caravan P., Bayes-Genis A., Libby P., Scadden D.T., Lin C.P., Naxerova K., Swirski F.K, & Nahrendorf M. (2021). Bone marrow endothelial dysfunction promotes myeloid cell expansion in cardiovascular disease. Nature cardiovascular research, 1(1), 28-44.
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3

Multiparametric Analysis of Immune Cells

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Anti-human antibodies included: anti-CD19-FITC from BD Biosciences; anti-CD24-PE (ML5) and anti-CD38-PE /Cy7 (HIT2) from Biolegend (San Diego, CA, USA); and Human FcR Binding Inhibitor from eBioscience (San Diego, CA, USA). Anti-mouse antibodies included: anti-CD4-FITC (RM4–5), anti-CD19-PE/Cy5, anti-CD19-APC (6D5), anti-CD25-APC (3C7), anti-IL-10-PE (JES5-16E3), PE Rat IgG2b, κ Isotype Ctrl (RTK4530), and anti-PD-L1-APC (10.F.9G2) from Biolegend; anti-CD1d-PE (1B1), anti-CD5-FITC (53–7.3), anti-CD16/CD32 (mouse Fc block), anti-Annexin-V-APC, and 7-AAD from BD Biosciences; and anti-CD25-PE/Cy5.5 (PC61.5), anti-Foxp3-PE (NRRF-30), anti-TLR4-Alexa Fluor® 488(UT41), and anti-FasL-FITC (MFL3) from eBioscience. Phosho-p38 MAPK (Thr180/Tyr182) (28B10) mouse mAb, p38 MAPK (D13E1) XP® rabbit mAb, phosho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® rabbit mAb, p44/42 MAPK (Erk1/2) (137F5) rabbit mAb, phosho-PI3K p85 (Tyr458)/p55 (Tyr199) antibody, PI3K p85α (6G10) mouse mAb, phospho-STAT1 (Thr701) (58D6) rabbit mAb, STAT1 (D1K9Y) rabbit mAb, GAPDH (14C10) rabbit mAb, β-Tubulin (9F3) rabbit mAB, anti-mouse IgG, HRP-linked antibody, anti-rabbit IgG, and HRP-linked antibody were obtained from Cell Signaling Technology (MA, USA); and anti-MyD88 antibody was obtained from Abcam (Cambridge, UK).
He Y., Yuan X., Li Y., Zhong C., Liu Y., Qian H., Xuan J., Duan L, & Shi G. (2018). Loss of Gαq impairs regulatory B-cell function. Arthritis Research & Therapy, 20, 186.
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