An isoflurane precision vaporizer (Smiths Medical PM) was used to anesthetize mice. Mice were then placed on a stereotaxic frame (David Kopf Instruments), with a Cunningham adaptor (Harvard Apparatus) to maintain anesthesia delivery during surgery. The skull was exposed, and a small hole was drilled at the desired injection site. The following stereotaxic coordinates were used: SNc, 3.1 and 1.3 mm posterior and lateral to bregma, at a depth of 4.5 mm from dura; dorsolateral striatum, 0.34 and 2.5 mm anterior and lateral to bregma, at a depth of 3 mm from dura. The Allen Mouse Brain Atlas, online version 1, 2008 (https://atlas.brain-map.org/ ) was used as a reference for the coordinates and generating diagrams.
For each mouse, the distance between bregma and lambda was calculated and used to adjust the coordinates. For AAV injections, ~350 nl of viral vector was delivered using a glass micropipette (Drummond Scientific) pulled with a P-97 glass puller (Sutter Instruments). Surgery for imaging experiments was performed unilaterally, and surgeries for RiboTag tissue collection were executed bilaterally. Experiments were performed after at least 10 postoperative days; tissue collection for RiboTag was performed 4 weeks after injection.
For each mouse, the distance between bregma and lambda was calculated and used to adjust the coordinates. For AAV injections, ~350 nl of viral vector was delivered using a glass micropipette (Drummond Scientific) pulled with a P-97 glass puller (Sutter Instruments). Surgery for imaging experiments was performed unilaterally, and surgeries for RiboTag tissue collection were executed bilaterally. Experiments were performed after at least 10 postoperative days; tissue collection for RiboTag was performed 4 weeks after injection.