Hq510lp

Manufactured by Chroma Technology

Sourced in Germany

The HQ510LP is a versatile laboratory equipment designed for various applications. It features a compact and durable construction, providing reliable performance in a wide range of research and testing environments.

Automatically generated - may contain errors

Lab products found in correlation

Poly l lysine solution, by Merck Group (1 mentions) Diaphot 300 inverted, by Nikon (1 mentions) 505dcxru, by Chroma Technology (1 mentions) Pdmi 2, by Harvard Apparatus (1 mentions) Imagem emccd camera, by Hamamatsu Photonics (1 mentions)

2 protocols using hq510lp

Fluorescence Imaging of Labeled Spermatozoa

Check if the same lab product or an alternative is used in the 5 most similar protocols

Fluorescence imaging was carried out as previously reported (Nishigaki et al., 2004 (link)). Briefly, Fluo-4-labeled spermatozoa were adhered to glass coverslips coated with 50 Pg/ml poly-L-lysine solution (Sigma) and mounted into a micro incubator, PDMI-2 (Harvard Apparatus, Holliston, MA, USA) maintained at 14 °C. Fluorescence images were acquired using a Nikon DIAPHOT 300 inverted microscope with a Nikon Plan Apo 60X objective lens (1.4 NA) and a custom-built stroboscopic illumination system (Nishigaki et al., 2006 (link)) with a Chroma filter set (Ex, HQ470/40x; DC, 505DCXRU; Em, HQ510LP (Chroma Technology)). Images were acquired with a Quantix 57 camera (Photometrics Inc.) under the continuous (stream) acquisition mode.

Beltrán C., Rodríguez-Miranda E., Granados-González G., de De la Torre L.G., Nishigaki T, & Darszon A. (2014). Zn2+ induces hyperpolarization by activation of a K+ channel and increases intracellular Ca2+ and pH in sea urchin spermatozoa. Developmental biology, 394(1), 15-23.

+ Open protocol

+ Expand

Live-cell Fluorescence Ratio Imaging

Check if the same lab product or an alternative is used in the 5 most similar protocols

Imaging was performed at 33 °C on our Olympus microscope with a 60x (1.42 NA) objective. A lambda DG-4 monochromator (Sutter Instruments) was used as the light source and to shift rapidly between CFP and YFP filters. The following filters were used: excitation, D436/10X and HQ485/15X; dichroic, 505DCXR; emission, HQ510lp; Chroma Technology, Olching Germany). Image acquisition was performed with an ImagEM EMCCD camera (Hamamatsu Photonics, Massy, France) controlled by MetaFluor software Series 7.8.9.0 (Roper Scientific, Evry, France). Sixteen-bit images (512 × 512) were acquired every 30 s for 5 min (acquisition time of 30 ms). After background substraction, the F480/F440 ratio was measured.

Côme E., Blachier S., Gouhier J., Russeau M, & Lévi S. (2023). Lateral Diffusion of NKCC1 Contributes to Chloride Homeostasis in Neurons and Is Rapidly Regulated by the WNK Signaling Pathway. Cells, 12(3), 464.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!