200,000 to 300,000 cells were seeded 24 h before PNDD0 or PNDD1 50 nM treatment. Three samples of the suspension cell culture were taken every 24 h after PNDD0 or PNDD1 50 nM treatment to measure the cell number and the percentage of dead cells. Three aliquots of supernatant were taken and mixed with equal volume of Trypan blue before reading on Countless Cell Automated Cell Counter (Invitrogen).
Countless cell automated cell counter
Manufactured by Thermo Fisher Scientific
The Countless Cell Automated Cell Counter is a laboratory equipment designed to count cells. It provides an automated and standardized method for determining the number of cells in a sample.
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Lab products found in correlation
2 protocols using countless cell automated cell counter
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Measuring Cell Growth and Viability
2
Cell Viability Assay for Targeting c-Myc
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200,000 to 300,000 cells were seeded 24 hours before PNDD0 or PNDD1 50 nM treatment.
Three samples of the suspension cell culture was taken every 24 hours after PNDD0 or PNDD1 50 nM treatment to measure the cell number and the percentage of dead cells.
Three aliquots of supernatant were taken and mixed with equal volume of Trypan blue before reading on Countless Cell Automated Cell Counter® (Invitrogen).
(which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission.
The copyright holder for this preprint this version posted April 7, 2020. ; https://doi.org/10.1101/2020.04.07.028977 doi: bioRxiv preprint PNDD1/tPE-H1 method to inhibit c-Myc is filled under the title and patent application : Chimeric Molecule for Targeting c-Myc in Cells. Patent Application No PCT/SG2018/050584.
Three samples of the suspension cell culture was taken every 24 hours after PNDD0 or PNDD1 50 nM treatment to measure the cell number and the percentage of dead cells.
Three aliquots of supernatant were taken and mixed with equal volume of Trypan blue before reading on Countless Cell Automated Cell Counter® (Invitrogen).
(which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission.
The copyright holder for this preprint this version posted April 7, 2020. ; https://doi.org/10.1101/2020.04.07.028977 doi: bioRxiv preprint PNDD1/tPE-H1 method to inhibit c-Myc is filled under the title and patent application : Chimeric Molecule for Targeting c-Myc in Cells. Patent Application No PCT/SG2018/050584.
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