Low melting point agarose

Manufactured by Sangon

Sourced in China

Low melting-point agarose is a type of agarose, a polysaccharide derived from red seaweed, that has a lower melting and gelling temperature compared to standard agarose. It is commonly used in gel electrophoresis applications.

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Lab products found in correlation

Dispase 2, by Merck Group (1 mentions) Dispase 2, by Corning (1 mentions) Streptavidin microbeads, by Miltenyi Biotec (1 mentions) Sf 900 2 sfm medium, by Thermo Fisher Scientific (1 mentions) Bac to bac expression system, by Thermo Fisher Scientific (1 mentions) Cellfectin 2 transfection reagent, by Thermo Fisher Scientific (1 mentions) Vibratome, by Leica (1 mentions) Geminisem 300, by Zeiss (1 mentions) Tcs sp8 confocal microscope, by Leica (1 mentions)

Spelling variants of this product

Agarose (33 citations) Low-melting agarose (8 citations)

4 protocols using low melting point agarose

Mangrove Sediment eDNA Extraction

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The sediment samples were collected from mangrove forest located in Longhai City, Fujian, China (Zini Mangrove Nature Reserve, 24°20′N, 117°45′E). About 50 g of sediments were collected in a 50 mL of sterile centrifugal tube and stored on ice. Stored the sediments samples in ultra-low temperature freezer (-80°C) after they were brought back to lab.
eDNA was extracted using the chemical lysis and enzyme digestion method. It was purified with low melting-point agarose [Sangon Biotech (Shanghai) Co., Ltd., China] and agarase (Takara Biotechnology Co., Ltd., Japan). Three replications of extraction and purification were performed and pooled together to avoid the extraction biases.

Qu W., Liu T., Wang D., Hong G, & Zhao J. (2018). Metagenomics-Based Discovery of Malachite Green-Degradation Gene Families and Enzymes From Mangrove Sediment. Frontiers in Microbiology, 9, 2187.

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Isolation of Mouse Alveolar Type II Cells

Cited 1 time

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Adult mouse lungs were used for the isolation of AT2 cells [11 (link)]. In brief, the lung was perfused with 4 °C PBS. After trachea intubation, dispase II (3 mg/mL, Corning, New York, NY, USA) was injected into the lung, followed by 1% low-melting-point agarose (42–45 °C, Sangon Biotech, Shanghai, China). Then, the lung was incubated in dispase II for 45 min at 37 °C and gently torn in DMEM/F12 + DNase I (0.01%, Sigma, St. Louis, MO, USA). The cell suspension was passed through serial filters (Solarbio, Beijing, China) and incubated with biotinylated antibodies: CD16/32 and CD45 (Miltenyi Biotec, Shanghai, China) for 10 min. After being incubated with 10 µL Streptavidin MicroBeads (4 °C, Miltenyi Biotec, Shanghai, China) for 15 min in dark, the cells were transferred onto plates pre-coated with 1 mg/mL IgG for 2 h, and the unattached cells were collected. The cell purity was detected by flow cytometry with corresponding antibodies.

Chen L., Yu T., Zhai Y., Nie H., Li X, & Ding Y. (2023). Luteolin Enhances Transepithelial Sodium Transport in the Lung Alveolar Model: Integrating Network Pharmacology and Mechanism Study. International Journal of Molecular Sciences, 24(12), 10122.

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Recombinant Baculovirus Production for RHDV2 VP60

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Spodoptera frugiperda Sf9 insect cells were maintained in Sf-900ⅡSFM medium (Gibco, MA, USA) at 28 °C. The plasmid pDEST10-VP60 was transformed into DH10Bac Escherichia coli competent cells, to generate recombinant bacmid DNA (Bac-to-bac expression system, Invitrogen, USA). The recombinant bacmid DNA was used to transfect Sf9 insect cells to generate the corresponding recombinant baculovirus, Bac-RHDV2-VP60, using Cellfectin II transfection reagent (Invitrogen, USA). Viral titres were determined by an end-point dilution assay (EPDA) using Sf9 cells. Subsequent infections were performed by adding virus to cells at a multiplicity of infection (MOI) of 10 TCID₅₀ units per cell. Cells were incubated for 2 h and then the medium was changed by fresh medium. Plaque purification was performed to isolate a pure viral stock. Briefly, the Sf9 cell monolayers were incubated with 10-fold serial dilutions of baculovirus Bac-RHDV2-VP60. After infection for 2 h, the medium containing virus was replaced by fresh medium, and the cells were overlaid with 1% low melting-point agarose (Sangon, ShangHai, China) in an Sf-900™ Medium (1.3×) medium (Gibco, USA) and incubated at 28 °C. After 7 days, several plaques were randomly picked from serial dilutions. Later the recombinant baculoviruses from individual plaques were amplified, titrated and then used to inoculate Sf9 cells.

Miao Q., Qi R., Veldkamp L., Ijzer J., Kik M.L., Zhu J., Tang A., Dong D., Shi Y., van Oers M.M., Liu G, & Pijlman G.P. (2019). Immunogenicity in Rabbits of Virus-Like Particles from a Contemporary Rabbit Haemorrhagic Disease Virus Type 2 (GI.2/RHDV2/b) Isolated in The Netherlands. Viruses, 11(6), 553.

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Multimodal Imaging of Lymph Node Microstructure

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Freshly dissected lymph nodes were immediately transferred to freshly prepared fixation buffer containing 4% PFA and 0.03% glutaraldehyde for 40 min at room temperature (RT), embedded in 2% (w/v) low‐melting point agarose (Sangon Biotech) in PBS. 50 μm sections were prepared with a Leica vibratome and placed in 35 mm glass bottom dishes for immunofluorescence staining. Images were acquired on a Leica TCS SP8 confocal microscope.
After the LM procedures, the samples were further fixed by immersion in 2.5% glutaraldehyde in 0.1 M phosphate buffer (pH 7.4) for 24 h at 4°C and washed in phosphate buffer. Samples were osmicated in 1% osmium containing 3% potassium ferricyanide (v/v) for 30 min at 4°C, incubated in 1% thiocarbohydrazide (TCH) (w/v) for 20 min at RT, followed by a second osmication in 1% osmium for 30 min at 4°C. Next, the samples were washed in ddH2O and incubated overnight in 1% aqueous uranyl acetate at 4°C. After final washing steps, the samples were then dehydrated stepwise through an ethanol series at 4°C, incubated in 50% epon resin for 2–3 h at RT, followed by an overnight incubation in 100% epon resin and subsequent embedding. Three‐dimensional SEM was carried out using scanning electron microscope (GeminiSEM 300, Carl ZEISS), scanning 70 nm ultrathin sections over a distance of 6 μm.

Chen X., Zheng Y., Liu S., Yu W, & Liu Z. (2022). CD169+ subcapsular sinus macrophage‐derived microvesicles are associated with light zone follicular dendritic cells. European Journal of Immunology, 52(10), 1581-1594.

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