Antibodies against lc3

Manufactured by Merck Group

Antibodies against LC3 are laboratory reagents used to detect and quantify the presence of the LC3 protein in biological samples. LC3 (Microtubule-associated protein 1A/1B-light chain 3) is a widely used marker for the process of autophagy, a cellular mechanism involved in the degradation and recycling of cellular components. These antibodies provide a tool for researchers to study the regulation and dynamics of autophagy in different cell types and experimental conditions.

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Lab products found in correlation

Beclin 1, by Cell Signaling Technology (2 mentions) Mouse anti rabbit igg antibody, by Santa Cruz Biotechnology (2 mentions) Acridine orange, by Merck Group (1 mentions) Monodansylcadaverine, by Merck Group (1 mentions) Mouse anti actin antibody, by Santa Cruz Biotechnology (1 mentions) Gapdh, by Santa Cruz Biotechnology (1 mentions) Bip grp78, by Cell Signaling Technology (1 mentions) Phospho eif2α ser51, by Cell Signaling Technology (1 mentions) Cycloheximide (chx), by Merck Group (1 mentions) Curcumin, by Merck Group (1 mentions) N acetyl cysteine (nac), by Merck Group (1 mentions) Z vad, by Merck Group (1 mentions) Caspase 3, by Cell Signaling Technology (1 mentions) Anti β actin, by Santa Cruz Biotechnology (1 mentions) Celltiter 96 aqueous one solution proliferation assay system, by Promega (1 mentions) Anti ubiquitin, by Santa Cruz Biotechnology (1 mentions) Ire1α, by Cell Signaling Technology (1 mentions) Hrp conjugated secondary antibody, by Santa Cruz Biotechnology (1 mentions) α tubulin, by Cell Signaling Technology (1 mentions) Anti puromycin antibody, by Merck Group (1 mentions)

Close spelling variants of this product

Polyclonal antibodies against LC3 (L7543) (2 citations) Antibodies against LC3 II (2 citations)

4 protocols using antibodies against lc3

Autophagy and Apoptosis Assays

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The following reagents were obtained commercially:
3‐[4,5‐dimethylthiazol‐2‐yl]2,5‐diphenyl tetrazolium bromide (MTT),
monodansylcadaverine (MDC), acridine orange were purchased from Sigma (St. Louis,
Missouri). 3‐Methyladenine (3‐MA, class III PI3K inhibitor) was obtained from
Calbiochem (La Jolla, California). Antibodies against the cleaved form of caspase‐3,
caspase‐8, Beclin‐1, and PARP were purchased from Cell Signaling Technology (Beverly,
MA). Antibodies against LC3 (Sigma) were also used. The p62/SQSTM1, caspase‐9,
ATG5‐ATG12 complex, GAPDH, mouse antiactin antibody, mouse antirabbit IgG antibody,
and rabbit antimouse IgG antibodies were purchased from Santa Cruz Biotechnology
(Santa Cruz, California). All other chemicals and reagents were purchased from Sigma
unless otherwise specified.

Lee Y., Park B., Park H., Yu S., Kang H, & Kim I. (2017). XIAP inhibitor embelin induces autophagic and apoptotic cell death in human oral squamous cell carcinoma cells. Environmental Toxicology, 32(11), 2371-2378.

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Curcumin Modulates Cellular Stress Responses

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Curcumin, AO, MDC, NAC, z-VAD, CHX, and DCF-DA were purchased from Sigma (St. Louis, MO, USA). The class III PI3K inhibitor 3-MA was obtained from Calbiochem (La Jolla, CA, USA). The CellTiter 96 AQueous One Solution Proliferation Assay System was purchased from Promega (Madison, WI, USA). Antibodies against PARP, caspase-3, p62, Beclin-1, CHOP, phospho-eIF2α (Ser51), IRE1-α, Bip/GRP78 and Nrf2 were purchased from Cell Signaling Technology (Beverly, MA, USA). Antibodies against LC3 (Sigma) were also used. The anti-β-actin, anti-ubiquitin, goat anti-rabbit and anti-mouse IgG antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA).

Lee W.J., Chien M.H., Chow J.M., Chang J.L., Wen Y.C., Lin Y.W., Cheng C.W., Lai G.M., Hsiao M, & Lee L.M. (2015). Nonautophagic cytoplasmic vacuolation death induction in human PC-3M prostate cancer by curcumin through reactive oxygen species -mediated endoplasmic reticulum stress. Scientific Reports, 5, 10420.

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Comprehensive Antibody Analysis in Cellular Signaling

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Antibodies against P-eIF2α (phosphorylated at Ser51), eIF2α, P-p38 (phosphorylated at Thr180/Tyr182), p-38, P-p70S6K (phosphorylated at Thr389), p70S6K, ADAM10, and α-tubulin were from Cell Signaling Technology. Antibodies against dynamin II were purchased from Santa-Cruz Biotechnology. Anti-Puromycin-antibody was from Merck Millipore. Antibodies against LC3 were bought from Sigma. HRP-conjugated secondary antibodies were from Santa-Cruz Biotechnology (mouse) and Cell Signaling Technology (rabbit).

von Hoven G., Neukirch C., Meyenburg M., Füser S., Petrivna M.B., Rivas A.J., Ryazanov A., Kaufman R.J., Aroian R.V, & Husmann M. (2015). eIF2α Confers Cellular Tolerance to S. aureus α-Toxin. Frontiers in Immunology, 6, 383.

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Autophagy Regulation in Osteogenesis

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Zoledronate, galangin, icariside II, kaempferol, quercetin, Zoledronate, galangin, icariside II, kaempferol, quercetin (Figure 1), and 3‐[4,5‐dimethylthiazol‐2‐yl]‐2,5‐diphenyl tetrazolium bromide reagents were purchased from Sigma (St. Louis, MO), and 3‐methyladenine (3‐MA; a type‐III phosphatidylinositol 3‐kinase [PI‐3 K] inhibitor) and rapamycin reagents were obtained from Calbiochem (La Jolla, CA). The antibodies against beclin‐1 and Autophagy protein 5 (ATG5) were purchased from Cell Signaling Technology (Beverly, MA). The antibodies against collagen I, osteocalcin, bone morphogenetic protein 2 (BMP‐2), Osterix, and RUNX2 were purchased from ABCam (Cambridge, MA). The antibodies against LC3 were purchased from Sigma. SQSTM1/p62, β‐actin antibody, mouse anti‐rabbit IgG antibody, and rabbit anti‐mouse IgG antibody were purchased from Santa Cruz Biotechnology (Santa Cruz, CA). All other chemicals and reagents were purchased from Sigma unless otherwise specified.

Kim J., Kang H., Yu S., Song J., Kim C., Kim B., Park B., Shin S, & Kim I. (2018). Cytoprotective effect of flavonoid‐induced autophagy on bisphosphonate mediated cell death in osteoblast. Journal of Cellular Biochemistry, 119(7), 5571-5580.

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