We prepared the CCI model as previously described by Bennett and Xie.13 (link) The rats in each group were anesthetized with chloral hydrate (300 mg/kg) before surgery. The left nervus ischiadicus was exposed at the middle of the thigh and ligated with four silk ligatures proximal to the trifurcation. In the sham group, the nervus ischiadicus was simply exposed without ligation. In the CCI group, however, the skin of the middle thigh of the left lower limb of rats was incised, and the nervus ischiadicus trunk was exposed. At 5 mm above the trifurcation, four ligations were made using 4–0 silk threads with a distance of 1–2 mm. Furthermore, the rats in the emodin group were administered emodin (Chengdu Must Bio-Technology Corporation, Purity: 99.48%, Lot number: MUST-16110712) via gavage once a day for 15 days (50 mg/kg) on the first day after the nerve ligation. emodin was dissolved to 6.25 g/L in 0.05% CMC-Na.
Emodin
Manufactured by Chengdu Must Bio-Technology
Sourced in China
Emodin is a chemical compound extracted from various plant sources. It serves as an analytical standard for high-performance liquid chromatography (HPLC) and other analytical techniques. Emodin is used to identify and quantify the presence of this compound in samples.
Automatically generated - may contain errors
Lab products found in correlation
Gallic acid, by Chengdu Must Bio-Technology (4 mentions)
Physcion, by Chengdu Must Bio-Technology (3 mentions)
Proanthocyanidin b1, by Chengdu Must Bio-Technology (2 mentions)
Proanthocyanidin b2, by Chengdu Must Bio-Technology (2 mentions)
Mili q water purification system, by Merck Group (2 mentions)
Rhein, by Chengdu Must Bio-Technology (2 mentions)
Formic acid hplc grade, by Tedia (2 mentions)
Catechin, by Chengdu Must Bio-Technology (1 mentions)
Methanol and acetonitrile hplc grade, by Merck Group (1 mentions)
Ammonium acetate, by Merck Group (1 mentions)
Hplc grade acetonitrile, by Merck Group (1 mentions)
Hplc grade methanol, by Merck Group (1 mentions)
Formic acid, by Shanghai Lingfeng Chemical Reagent (1 mentions)
Alexa fluor 555 labeled donkey anti mouse igg h l, by Beyotime (1 mentions)
2 3 5 triphenyltetrazolium chloride, by Merck Group (1 mentions)
Anti gapdh, by Affinity Biosciences (1 mentions)
Il 6 antibody, by Abcam (1 mentions)
Talent qpcr premix sybr green, by Tiangen Biotech (1 mentions)
Anti occludin, by Abcam (1 mentions)
Vegfa antibody, by Affinity Biosciences (1 mentions)
8 protocols using emodin
1
Chronic Constriction Injury Model and Emodin Treatment
2
Authentication and Chemical Standards for Polygonum multiflorum
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The PMR materials were collected from three different habitats in China, and three replicate sample materials were from different biological plants (Table 4 ). All samples were authenticated as the tuberous roots of Polygonum multiflorum Thunb. by Prof. Zhong-Zhen Zhao from the School of Chinese Medicine, Hong Kong Baptist University. Voucher specimens were deposited in the Bank of China (Hong Kong) Chinese Medicines Centre of Hong Kong Baptist University.
Chemical standards for trans-THSG, emodin, physcion, catechin, proanthocyanidin B1, proanthocyanidin B2, and gallic acid were purchased from Chengdu Must Bio-Technology Co., Ltd. (Chengdu, China); standards for cis-THSG, epicatechin, epicatechin-3-gallate, emodin-8-O-β-D -glucoside, and physcion-8-O-β-D -glucoside were purchased from Chengdu Xunchen Biological Technology Co., Ltd. (Chengdu, China). HPLC-grade methanol and acetonitrile (E. Merck, Darmstadt, Germany), ammonium acetate (Sigma-Aldrich, St. Louis, MO, USA), HPLC grade formic acid (Tedia, Fairfield, OH, USA) were purchased. Ultra-pure water was prepared by a Mili-Q water purification system (Millipore, Burlington, MA, USA).
Chemical standards for trans-THSG, emodin, physcion, catechin, proanthocyanidin B1, proanthocyanidin B2, and gallic acid were purchased from Chengdu Must Bio-Technology Co., Ltd. (Chengdu, China); standards for cis-THSG, epicatechin, epicatechin-3-gallate, emodin-8-O-β-
3
Authentication and Characterization of Polygonum multiflorum
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
RPMR materials were collected from Kaili City (Guizhou, China), the geo-authentic producing area of PMR, and were authenticated as the tuberous roots of Polygonum multiflorum Thunb. by Prof. Zhongzhen Zhao. The voucher specimens were deposited in the Chinese Medicines Centre, Hong Kong Baptist University.
Chemical standards including gallic acid, proanthocyanidin B1, proanthocyanidin B2, trans-2,3,5,4′-tetrahydroxylstilbene-2-O-β-D-glucoside (trans-THSG), emodin, physcion were ordered from Chengdu Must Bio-Technology Co., Ltd. (Chengdu, China); epicatechin, epicatechin-gallate, emodin-8-O-β-D-glucoside, physcion-8-O-β-D-glucoside were supplied by Chengdu Xunchen Biological Technology Co., Ltd. (Chengdu, China). Acetonitrile (HPLC grade) and methanol (HPLC grade) were supplied by E. Merck (Darmstadt, Germany); ammounium acetate (Sigma-aldrich, USA) was purchased; formic acid (HPLC grade) was purchased from Tedia (USA); ultra-pure water was prepared by a Mili-Q water purification system (Millipore, MA, USA).
Chemical standards including gallic acid, proanthocyanidin B1, proanthocyanidin B2, trans-2,3,5,4′-tetrahydroxylstilbene-2-O-β-D-glucoside (trans-THSG), emodin, physcion were ordered from Chengdu Must Bio-Technology Co., Ltd. (Chengdu, China); epicatechin, epicatechin-gallate, emodin-8-O-β-D-glucoside, physcion-8-O-β-D-glucoside were supplied by Chengdu Xunchen Biological Technology Co., Ltd. (Chengdu, China). Acetonitrile (HPLC grade) and methanol (HPLC grade) were supplied by E. Merck (Darmstadt, Germany); ammounium acetate (Sigma-aldrich, USA) was purchased; formic acid (HPLC grade) was purchased from Tedia (USA); ultra-pure water was prepared by a Mili-Q water purification system (Millipore, MA, USA).
4
Extraction and Identification of Bioactive Compounds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The ionic liquids used in the study included 1-butyl-3-methylimidazolium hexafluorophosphate ([Bmim]PF6), 1-dodecyl-3-methyl chloride imidazole ([Domim]Cl), 1-dodecyl-3-methyl-1-H-imidazolium hydrogensulfate ([Domim]HSO4), 1-dodecyl-3-methyl-1-H-imidazolium nitrate ([Domim]NO3), were offered by Chengjie Chemical Co., Ltd. (Shanghai, China). C18 and silica gel were purchased from Shanghai Chengya Chemical Co., Ltd. (Shanghai, China). Standard substances including aurantio-obtusin, chryso-obtusin, obtusifolin and emodin were supplied from Chengdu Must Bio-Technology Co., Ltd. (Chengdu, China). Their purities were all higher than 98% and their structures are shown in Figure 7 . Purified water was purchased from Wahaha Group Ltd. (Shanghai, China). HPLC grade methanol and acetonitrile were obtained from American Tedia Co., Ltd. (Shanghai, China). Formic acid was of analytical reagent grade from Shanghai Lingfeng Chemical Reagent Co., Ltd. (Shanghai, China). All reagents for HPLC were filtrated through a 0.22 μm filter.
5
Emodin Regulates Neuroinflammation Pathways
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The following
materials and reagents were used in the study: Emodin (no.: A0044,
Chengdu Must Biotechnology Co., Ltd., China, purity≥98%); anti-MMP9
(no.: Ab38898, Abcam, U.K.); anti-NF-κB p65 (no.: #8242, CST,
USA); anti-IKKβ (no.: #2678, CST, USA); 2,3,5-triphenyl tetrazolium
chloride (no.: T8877-25G, Sigma, USA); hematoxylin and eosin (HE)
staining kit (no.:BP-DL017, SenBeiJia Biological Technology Co., Ltd.
China); HRP-labeled goat anti-rabbit IgG (H + L) (no.: A0208, Beyotime
Biotechnology, China); anti-MMP2 (no.: Ab181286), anti-occludin (no.:
Ab216327), anti-extracellular signal-regulated kinase [ERK1/2 (no.:
Ab184699)], and p-anti-ERK1/2 (no.: Ab214036)—Abcam, U.K.;
IL-6 antibody (no.: DF6087), HIF1A antibody (no./AF1009), claudin-5
(no./AF5216), and VEGFA antibody (no.: DF7470)—Affinity, China;
Alexa Fluor 555-labeled donkey anti-mouse IgG(H + L) (A0460), Triton
X-100 (no.: ST795), and antifade mounting medium (no.: P0126)—Beyotime
Biotechnology, China; neutral balsam (no.: G8590, Beijing Solarbio
Science & Technology Co., Ltd. China); RNA Easy Fast Tissue/Cell
Kit (no.: DP451), FastKing gDNA Dispelling RT SuperMix (no.: KR118),
and Talent qPCR PreMix (SYBR Green) (no.: FP209)—Tiangen Biotech
(Beijing) Co., Ltd, China; anti-GAPDH (no: AF7021, Affinity); and
BCA Protein Assay Kit (no: P0012S, Beyotime Biotechnology, China).
materials and reagents were used in the study: Emodin (no.: A0044,
Chengdu Must Biotechnology Co., Ltd., China, purity≥98%); anti-MMP9
(no.: Ab38898, Abcam, U.K.); anti-NF-κB p65 (no.: #8242, CST,
USA); anti-IKKβ (no.: #2678, CST, USA); 2,3,5-triphenyl tetrazolium
chloride (no.: T8877-25G, Sigma, USA); hematoxylin and eosin (HE)
staining kit (no.:BP-DL017, SenBeiJia Biological Technology Co., Ltd.
China); HRP-labeled goat anti-rabbit IgG (H + L) (no.: A0208, Beyotime
Biotechnology, China); anti-MMP2 (no.: Ab181286), anti-occludin (no.:
Ab216327), anti-extracellular signal-regulated kinase [ERK1/2 (no.:
Ab184699)], and p-anti-ERK1/2 (no.: Ab214036)—Abcam, U.K.;
IL-6 antibody (no.: DF6087), HIF1A antibody (no./AF1009), claudin-5
(no./AF5216), and VEGFA antibody (no.: DF7470)—Affinity, China;
Alexa Fluor 555-labeled donkey anti-mouse IgG(H + L) (A0460), Triton
X-100 (no.: ST795), and antifade mounting medium (no.: P0126)—Beyotime
Biotechnology, China; neutral balsam (no.: G8590, Beijing Solarbio
Science & Technology Co., Ltd. China); RNA Easy Fast Tissue/Cell
Kit (no.: DP451), FastKing gDNA Dispelling RT SuperMix (no.: KR118),
and Talent qPCR PreMix (SYBR Green) (no.: FP209)—Tiangen Biotech
(Beijing) Co., Ltd, China; anti-GAPDH (no: AF7021, Affinity); and
BCA Protein Assay Kit (no: P0012S, Beyotime Biotechnology, China).
6
Aspirin and Herbal Compound ELISA Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Aspirin was purchased from by Bayer HealthCare with the batch number of J20130078). Enzyme-linked Immunosorbent Assay (ELISA) Kits including Rat interleukin 1 (IL-1), Rat S-100B protein, Rat nuclear factorκB (NF-κB), Rat neuron specific enolase (NSE), Rat high sensitive C reactive protein (HS-CRP), Rat estradiol (E2), Rat tumor necrosis factor-α (TNF-α), and Rat D-dimmer (D2D), were purchased from Nanjing Senbeijia Biological Technology Co., Ltd., Trizol was purchased from Invitrogen. Anti-AKT, Anti-PI3K, and Anti-β-actin antibody was all purchased from Abcam. All durgs (purity assay by HPLC ≥ 98%, power) including compounds of Emodin, Ginsenoside Rg1, Ginsenoside Rb1, Notoginsenoside R1, Baicalein, Geniposide, β-asarone, Taurine, Paeoniflorin, Paeonol, Apigenin, Rhein, and Catalpol, were purchased from Chengdu Must Bio-Technology Co., Ltd.
7
Simultaneous Quantification of Seven Compounds in SK Injection
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Authentic standards of gallic acid, danshensu, rosmarinic acid, salvianolic acid A, aloe-emodin, rhein and emodin were obtained from Chengdu Must Bio-technology Co., Ltd. (Sichuan, China). Carbamazepine using as internal standard was purchased from National Institute for the Control of Pharmaceutical and Biological Products (Beijing, China). These compounds were determined with the purity >98% by HPLC or GC method. The medicines of SK injection were bought from Xian Shiji Shengkang Pharmaceutical Industry Co., Ltd. (Xian, Shannxi, China).
Methanol and acetonitrile (chromatographic grade) were purchased from Fisher Scientific (Pittsburgh, PA, USA). LC-MS grade formic acid was obtained from Aladdin Industrial Co., Ltd. (Shanghai, China). All the other chemicals were analytical grade. The deionized water (18.2 MΩ) was purified by a Millipore water purification system (Millipore, Shanghai, China) and all of the solutions were filtrated by 0.22 μm pore size filters.
Methanol and acetonitrile (chromatographic grade) were purchased from Fisher Scientific (Pittsburgh, PA, USA). LC-MS grade formic acid was obtained from Aladdin Industrial Co., Ltd. (Shanghai, China). All the other chemicals were analytical grade. The deionized water (18.2 MΩ) was purified by a Millipore water purification system (Millipore, Shanghai, China) and all of the solutions were filtrated by 0.22 μm pore size filters.
8
Comprehensive Phytochemical Characterization of PMRP
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
HPLC grade acetonitrile (ACN) and acetic acid were purchased from Sigma-Aldrich (St. Louis, MO, USA), and Dikma Technology Co., Ltd. (Beijing, China). Ethanol (industrial grade) was obtained from Shandong Yuwang Industry Co., Ltd. (Shandong, China). Purified water was obtained from the Hangzhou Wahaha Corporation (Hangzhou, China). PMRP was bought from GuoDa Pharmacy (Shenyang, Liaoning, China) and was identified as the processed root of Polygonum multiflorum Thunb. by professor Zhiguo Yu of the Shenyang Pharmaceutical University. The reference standards of emodin, physcion, and gallic acid were purchased from Chengdu MUST Bio-Technology Co., Ltd. (Sichuan, China). Chrysophanol, rhein and polydatin were obtained from the National Institute for the Control of Pharmaceutical and Biological Products (NICPBP, Beijing, China). emodin-8-O-β-d -glucoside and 2,3,5,4-tetrahydroxystilbene-2-O-β-d -glucoside (THSG) were obtained from Sichuan Victory Bio-Technology Co., Ltd. (Sichuan, China). The purity of all the reference substances was higher than 98%.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!