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Protein purification kit

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The Protein Purification Kit is a laboratory equipment designed to isolate and purify proteins from biological samples. It contains all the necessary components for the protein extraction, purification, and concentration process. The kit utilizes affinity chromatography techniques to selectively capture and separate the target proteins from complex mixtures.

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Lab products found in correlation

Bl21 codonplus de3 ripl, by Agilent Technologies (4 mentions) Transcend non radioactive translation detection system, by Promega (2 mentions) Tnt quick coupled transcription translation kit, by Promega (2 mentions) Lrrk2, by Sino Biological (2 mentions) Recombinant flag gpnmb, by OriGene (2 mentions) Gst egfr, by Active Motif (2 mentions) Recombinant active caspase 1, by R&D Systems (2 mentions) Hif 1α, by Novus Biologicals (2 mentions) Quikchange lightning site directed mutagenesis kit, by Agilent Technologies (2 mentions) Gateway system, by Thermo Fisher Scientific (2 mentions) Pcdna dest40, by Thermo Fisher Scientific (2 mentions) Pinducer20 inducible lentiviral expression vector, by Thermo Fisher Scientific (2 mentions)

4 protocols using protein purification kit

1

Recombinant Protein Expression and Purification

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Recombinant proteins were expressed in E.coli strain BL21-CodonPlus® (DE3)-RIPL (Agilent Technologies) and purified using Protein Purification Kit (Clontech). Recombinant Flag-GPNMB and PHD1 were purchased from Origene. GST-EGFR was purchased from Active Motif, HIF1α and BRK were purchased from Novus Biologicals. LRRK2 was purchased from SignalChem. Recombinant active Caspase-1 was purchased from R&D Systems. In vitro translation of LINK-A was conducted using TnT® Quick Coupled Transcription/Translation Kit and detection was performed using Transcend™ Non-Radioactive Translation Detection System (Promega).
Lin A., Li C., Xing Z., Hu Q., Liang K., Han L., Wang C., Hawke D.H., Wang S., Zhang Y., Wei Y., Ma G., Park P.K., Zhou J., Zhou Y., Hu Z., Zhou Y., Marks J.R., Liang H., Hung M.C., Lin C, & Yang L. (2016). The LINK-A lncRNA Activates Normoxic HIF1α Signaling in Triple-negative Breast Cancer. Nature cell biology, 18(2), 213-224.
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2

Recombinant Protein Expression and Purification

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Recombinant proteins were expressed in E.coli strain BL21-CodonPlus® (DE3)-RIPL (Agilent Technologies) and purified using Protein Purification Kit (Clontech). Recombinant Flag-GPNMB and PHD1 were purchased from Origene. GST-EGFR was purchased from Active Motif, HIF1α and BRK were purchased from Novus Biologicals. LRRK2 was purchased from SignalChem. Recombinant active Caspase-1 was purchased from R&D Systems. In vitro translation of LINK-A was conducted using TnT® Quick Coupled Transcription/Translation Kit and detection was performed using Transcend™ Non-Radioactive Translation Detection System (Promega).
Lin A., Li C., Xing Z., Hu Q., Liang K., Han L., Wang C., Hawke D.H., Wang S., Zhang Y., Wei Y., Ma G., Park P.K., Zhou J., Zhou Y., Hu Z., Zhou Y., Marks J.R., Liang H., Hung M.C., Lin C, & Yang L. (2016). The LINK-A lncRNA Activates Normoxic HIF1α Signaling in Triple-negative Breast Cancer. Nature cell biology, 18(2), 213-224.
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3

Mammalian and Bacterial Expression Vectors

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Mammalian expression vectors for full-length LINK-A and the deletion mutant were constructed by subcloning the gene sequences into a pCDNA3.1 (+) backbone and pInducer20 inducible lentiviral expression vector (Life Technologies). Mammalian expression of full-length TRIM71, PKA C-α, CNR2, GABR1, ADA2A, ACM4, OPRM, and mutant vectors were constructed by subcloning the corresponding gene sequences into the His-tagged expression vector (pcDNA™-DEST40) using the Gateway system (Life Technologies). Bacteria expression of full-length CNR2, GABR1, ADA2A, ACM4, OPRM, and mutant vectors was constructed by subcloning the corresponding gene sequences into the GST-tagged expression vector pGEX-5X-1. All single-point and deletion mutations were generated using the QuikChange Lightning Site-Directed Mutagenesis Kit (Agilent Technologies). Recombinant proteins were expressed in the Escherichia coli strain BL21-CodonPlus (DE3)-RIPL (Agilent Technologies) and purified using the Protein Purification Kit (Clontech).
Hu Q., Ye Y., Chan L.C., Li Y., Liang K., Lin A., Egranov S.D., Zhang Y., Xia W., Gong J., Pan Y., Chatterjee S.S., Yao J., Evans K.W., Nguyen T.K., Park P.K., Liu J., Coarfa C., Donepudi S.R., Putluri V., Putluri N., Sreekumar A., Ambati C.R., Hawke D.H., Marks J.R., Gunaratne P.H., Caudle A.S., Sahin A.A., Hortobagyi G.N., Meric-Bernstam F., Chen L., Yu D., Hung M.C., Curran M.A., Han L., Lin C, & Yang L. (2019). Oncogenic LncRNA downregulates cancer cell antigen presentation and intrinsic tumor suppression. Nature immunology, 20(7), 835-851.
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4

Mammalian and Bacterial Expression Vectors

Check if the same lab product or an alternative is used in the 5 most similar protocols
Mammalian expression vectors for full-length LINK-A and the deletion mutant were constructed by subcloning the gene sequences into a pCDNA3.1 (+) backbone and pInducer20 inducible lentiviral expression vector (Life Technologies). Mammalian expression of full-length TRIM71, PKA C-α, CNR2, GABR1, ADA2A, ACM4, OPRM, and mutant vectors were constructed by subcloning the corresponding gene sequences into the His-tagged expression vector (pcDNA™-DEST40) using the Gateway system (Life Technologies). Bacteria expression of full-length CNR2, GABR1, ADA2A, ACM4, OPRM, and mutant vectors was constructed by subcloning the corresponding gene sequences into the GST-tagged expression vector pGEX-5X-1. All single-point and deletion mutations were generated using the QuikChange Lightning Site-Directed Mutagenesis Kit (Agilent Technologies). Recombinant proteins were expressed in the Escherichia coli strain BL21-CodonPlus (DE3)-RIPL (Agilent Technologies) and purified using the Protein Purification Kit (Clontech).
Hu Q., Ye Y., Chan L.C., Li Y., Liang K., Lin A., Egranov S.D., Zhang Y., Xia W., Gong J., Pan Y., Chatterjee S.S., Yao J., Evans K.W., Nguyen T.K., Park P.K., Liu J., Coarfa C., Donepudi S.R., Putluri V., Putluri N., Sreekumar A., Ambati C.R., Hawke D.H., Marks J.R., Gunaratne P.H., Caudle A.S., Sahin A.A., Hortobagyi G.N., Meric-Bernstam F., Chen L., Yu D., Hung M.C., Curran M.A., Han L., Lin C, & Yang L. (2019). Oncogenic LncRNA downregulates cancer cell antigen presentation and intrinsic tumor suppression. Nature immunology, 20(7), 835-851.
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