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Rat anti mouse iga antibody

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The Rat anti-mouse IgA antibody is a laboratory reagent used to detect and quantify the presence of mouse immunoglobulin A (IgA) in various samples. It is a specific antibody produced in rats that binds to mouse IgA molecules, allowing for their identification and measurement.

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Lab products found in correlation

Mounting medium, by Vector Laboratories (2 mentions) Rat anti mouse aid antibody, by Thermo Fisher Scientific (2 mentions) Goat fitc labeled anti rat igg antibody, by Jackson ImmunoResearch (2 mentions) 4 6 diamidino 2 phenylindole (dapi), by Vector Laboratories (2 mentions)

Close spelling variants of this product

Rat anti-mouse IgA (5 citations) Anti-mouse IgA (2 citations) Mouse anti (2 citations)

2 protocols using rat anti mouse iga antibody

1

Quantifying Intestinal IgA and AID

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To unmask antigens, small intestinal sections were boiled in Antigen Unmasking Solution (C, Inc. Burlingame, CA). Then sections were blocked using 10% goat serum and stained with a rat anti-mouse IgA antibody (BD Pharmingen) and a rat anti-mouse AID antibody (eBioscience) overnight at 4°C, followed by a goat FITC-labeled anti-rat IgG antibody (Jackson ImmunoResearch, West Grove, PA) for 1 hour at room temperature. Sections were then mounted using Mounting Medium containing DAPI (Vector laboratories) for nuclear counter-staining and observed using fluorescence microscopy. FITC and DAPI images were taken from the same field. Positive cells in 500 vili in ileum were counted.
Wang Y., Liu L., Moore D.J., Shen X., Peek RM J.r., Acra S.A., Li H., Ren X., Polk D.B, & Yan F. (2016). A LGG-derived protein promotes IgA production through up-regulation of APRIL expression in intestinal epithelial cells. Mucosal immunology, 10(2), 373-384.
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2

Quantifying Intestinal IgA and AID

Check if the same lab product or an alternative is used in the 5 most similar protocols
To unmask antigens, small intestinal sections were boiled in Antigen Unmasking Solution (C, Inc. Burlingame, CA). Then sections were blocked using 10% goat serum and stained with a rat anti-mouse IgA antibody (BD Pharmingen) and a rat anti-mouse AID antibody (eBioscience) overnight at 4°C, followed by a goat FITC-labeled anti-rat IgG antibody (Jackson ImmunoResearch, West Grove, PA) for 1 hour at room temperature. Sections were then mounted using Mounting Medium containing DAPI (Vector laboratories) for nuclear counter-staining and observed using fluorescence microscopy. FITC and DAPI images were taken from the same field. Positive cells in 500 vili in ileum were counted.
Wang Y., Liu L., Moore D.J., Shen X., Peek RM J.r., Acra S.A., Li H., Ren X., Polk D.B, & Yan F. (2016). A LGG-derived protein promotes IgA production through up-regulation of APRIL expression in intestinal epithelial cells. Mucosal immunology, 10(2), 373-384.
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